The three isoforms of hepcidin in human serum and their processing determined by liquid chromatography-tandem mass spectrometry (LC-tandem MS)

International Journal of Hematology

Volumn 103, Issue 1, 2016, Pages 34-43

  Addo, Lynda ^a   Ikuta, Katsuya ^a   Tanaka, Hiroki ^a   Toki, Yasumichi ^a   Hatayama, Mayumi ^a   Yamamoto, Masayo ^a   Ito, Satoshi ^a   Shindo, Motohiro ^a   Sasaki, Yusuke ^b   Shimonaka, Yasushi ^b   Fujiya, Mikihiro ^a   Kohgo, Yutaka ^a  

^a ASAHIKAWA MEDICAL COLLEGE   (Japan)

^b CHUGAI PHARMACEUTICAL CO LTD   (Japan)

Author keywords

Hepcidin; Hepcidin isoforms; LC Tandem MS

Indexed keywords

CREATININE; FERRITIN; HEPCIDIN; HEPCIDIN 20; HEPCIDIN 22; HEPCIDIN 25; UNCLASSIFIED DRUG; ISOPROTEIN;

ARTICLE; BLOOD SAMPLING; CHRONIC KIDNEY DISEASE; CORRELATIONAL STUDY; CREATININE BLOOD LEVEL; FERRITIN BLOOD LEVEL; HEPG2 CELL LINE; HUMAN; HUMAN CELL; INVESTIGATIVE PROCEDURES; LIQUID CHROMATOGRAPHY; LIVER CELL; NORMAL HUMAN; TANDEM MASS SPECTROMETRY; BLOOD; IRON DEFICIENCY ANEMIA; IRON OVERLOAD;

ANEMIA, IRON-DEFICIENCY; CHROMATOGRAPHY, LIQUID; HEPCIDINS; HUMANS; IRON OVERLOAD; PROTEIN ISOFORMS; TANDEM MASS SPECTROMETRY;

EID: 84954401168     PISSN: 09255710     EISSN: 18653774     Source Type: Journal    
DOI: 10.1007/s12185-015-1885-y     Document Type: Article

References (36)

1. Aisen P, Enns C, Wessling-Resnick M. Chemistry and biology of eukaryotic iron metabolism. Int J Biochem Cell Biol. 2001;33:940–59.
2. Andrews NC. Disorders of iron metabolism. N Engl J Med. 1999;341:1986–95.
3. Pigeon C, Ilyin G, Courselaud B, Leroyer P, Turlin B, Brissot P, et al. A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload. J Biol Chem. 2001;276:7811–9.
4. Nicolas G, Viatte L, Bennoun M, Beaumont C, Kahn A, Vaulont S. Hepcidin, a new iron regulatory peptide. Blood Cells Mol Dis. 2002;29:327–35.
5. Ganz T. Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation. Blood. 2003;102:783–8.
6. Krause A, Neitz S, Magert HJ, Schultz A, Forssmann WG, Schultz-Knappe P, et al. LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity. FEBS Lett. 2000;480:147–50.
7. Park CH, Valore EV, Waring AJ, Ganz T. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J Biol Chem. 2001;276:7806–10.
8. Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM, et al. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science. 2004;306:2090–3.
9. De Domenico I, Nemeth E, Nelson JM, Phillips JD, Ajioka RS, Kay MS, et al. The hepcidin-binding site on ferroportin is evolutionarily conserved. Cell Metab. 2008;8:146–56.
10. Mei S, Wang H, Fu R, Qu W, Xing L, Wang G, et al. Hepcidin and GDF15 in anemia of multiple myeloma. Int J Hematol. 2014;100:266–73.
11. Kroot JJC, Tjalsma H, Fleming RE, Swinkels DW. Hepcidin in human iron disorders: diagnostic implications. Clin Chem. 2011;57:1650–69.
12. Maisetta G, Petruzzelli R, Brancatisano FL, Essin S, Vitali A, Campa M, et al. Antimicrobial activity of human hepcidin-20 and 25 against clinically relevant bacterial strains: effect of copper and acidic pH. Peptides. 2010;31:1995–2002.
13. Tavanti A, Maisetta G, Del Gaudio G, Petruzelli R, Sanguinetti M, Batoni G, et al. Fungicidal activity of the human peptide hepcidin 20 alone or in combination with other antifungals against Candida glabrata isolates. Peptides. 2011;32:2484–7.
14. Piperno A, Mariani R, Trombini P, Girelli D. Hepcidin modulation in human diseases: from research to clinic. World J Gastroenterol. 2009;15:538–51.
15. Kemna E, Tjalsma H, Podust VN, Swinkels DW. Mass spectrometry-based hepcidin measurements in serum and urine: analytical aspects and clinical implications. Clin Chem. 2007;53:620–8.
16. Ganz T, Olbina G, Girelli D, Nemeth E, Westerman M. Immunoassay for huma serum hepcidin. Blood. 2008;112:4292–7.
17. Tomosugi N, Kawabata H, Wakatabe R, Higuchi M, Yamaya H, Umehara H, et al. Detection of serum hepcidin in renal failure and inflammation by using ProteinChip system. Blood. 2006;108:1381–7.
18. Campostrini N, Castagna A, Zaninotto F, Bedogna V, Tessitore N, Poli A, et al. Evaluation of hepcidin isoforms in hemodialysis patients by a proteomic approach based on SELDI-TOF MS. J Biomed Biotechnol. 2010;2010:329646.
19. Girelli D, De Domenico I, Bozzini C, Campostrini N, Busti F, Castagna A et al. Clinical, pathological, and molecular correlates in ferroportin disease: a study of two novel mutations. J Hepatol. 2008;49:664–71.
20. Kemna E, Tjalsma H, Laarakkers C, Nemeth E, Willems H, Swinkels D. Novel urine hepcidin assay by mass spectrometry. Blood. 2005;106:3268–70.
21. Swinkels DW, Girelli D, Laarakkers C, Kroot J, Campostrini N, Kemna EH, et al. Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry. PLoS One. 2008;3:e2706.
22. Hosoki T, Ikuta K, Shimonaka Y, Sasaki Y, Yasuno H, Sato K, et al. Heterogeneous expressions of hepcidin isoforms in hepatoma-derived cells detected using simultaneous LC-MS/MS. Proteomics Clin Appl. 2009;3:1256–64.
23. Sasaki Y, Shimonaka Y, Ikuta K, Hosoki T, Sasaki K, Torimoto Y, et al. Hepcidin production in response to iron is controlled by monocyte-derived humoral factors. Int J Hematol. 2014;99:12–20.
24. Malyszko J. Hepcidin assays: ironing out some details. Clin J Am Soc Nephrol. 2009;4:1015–6.
25. Rossi E. Hepcidin-the iron regulatory hormone. Clin Biochem Rev. 2005;26:47–9.
26. Ganz T, Nemeth E. Iron imports IV. Hepcidin and regulation of body iron metabolism. Am J Physiol Gastrointest Liver Physiol. 2006;290:G199–203.
27. Leong WI, Lönnerdal B. Hepcidin, the recently identified peptide that appears to regulate iron absorption. J Nutr. 2004;134:1–4.
28. Kemna EH, Tjalsma H, Willems HL, Swinkels DW. Hepcidin: from discovery to differential diagnosis. Haematologica. 2008;93:90–7.
29. Lin L, Valore EV, Nemeth E, Goodnough JB, Gabayan V, Ganz T. Iron transferrin regulates hepcidin synthesis in primary hepatocyte culture through haemojuvelin and BMP2/4. Blood. 2007;110:2182–9.
30. Ganz T. Hepcidin—a regulator of intestinal iron absorption and iron recycling by macrophages. Best Pract Res Clin Haematol. 2005;18:171–82.
31. Roe MA, Collings R, Dainty JR, Swinkels DW, Fairweather-Tait SJ. Plasma hepcidin concentrations significantly predict interindividual variation in iron absorption in healthy men. Am J Clin Nutr. 2009;89:1088–91.
32. Guo X, Zhou D, An P, Wu Q, Wang H, Wu A, et al. Associations between serum hepcidin, ferritin and Hb concentrations and type 2 diabetes risks in a Han Chinese population. Br J Nutr. 2013;110:2180–5.
33. Campostrini N, Traglia M, Martinelli N, et al. Serum levels of the hepcidin-20 isoform in a large general population: the Val Borbera study. J Proteomics. 2012;76:28–35.
34. Kroot JJ, Laarakkers CM, Geurts-Moespot AJ, Grebechtchikov N, Pickkers P, van Ede AE, et al. Immunochemical and mass-spectrometry-based serum hepcidin assays for iron metabolism disorders. Clin Chem. 2010;56:1570–9.
35. Suzuki H, Toba K, Kato K, Ozawa T, Tomosugi N, Higuchi M, et al. Serum hepcidin-20 is elevated during the acute phase of myocardial infarction. Tohoku J Exp Med. 2009;218:93–8.
36. Laarakkers CMM, Wiegerinck ET, Klaver S, Kolodziejczyk M, Gille H, Hohlbaum AM, et al. Improved mass spectrometry assay for plasma hepcidin: detection and characterization of a novel hepcidin isoform. PLoS One. 2013;8:e75518.