Improved Mass Spectrometry Assay For Plasma Hepcidin: Detection and Characterization of a Novel Hepcidin Isoform

PLoS ONE

Volumn 8, Issue 10, 2013, Pages

  Laarakkers, Coby M M ^a,b   Wiegerinck, Erwin T ^a,b   Klaver, Siem ^a,b   Kolodziejczyk, Maria ^c   Gille, Hendrik ^c   Hohlbaum, Andreas M ^c   Tjalsma, Harold ^a,b   Swinkels, Dorine W ^a,b  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^b Hepcidinanalysiscom   (Netherlands)

^c Pieris Pharmaceuticals GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FERROPORTIN; HEPCIDIN; HEPCIDIN 20; HEPCIDIN 22; HEPCIDIN 24; HEPCIDIN 25; ISOPROTEIN; ISOTOPE; UNCLASSIFIED DRUG;

ARTICLE; BIOASSAY; BLOOD STORAGE; CATION EXCHANGE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; HORMONE DETERMINATION; HORMONE STRUCTURE; HUMAN; INTERMETHOD COMPARISON; KIDNEY DISEASE; MOLECULAR WEIGHT; PLASMA; PROCESS OPTIMIZATION; PROTEIN DEGRADATION; PROTEIN DETERMINATION; QUANTITATIVE ANALYSIS; ROOM TEMPERATURE; STANDARD; TIME OF FLIGHT MASS SPECTROMETRY; VALIDATION PROCESS;

HEPCIDINS; HUMANS; MASS SPECTROMETRY; PROTEIN ISOFORMS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84885023832     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0075518     Document Type: Article

References (37)

1. Kroot JJ, Tjalsma H, Fleming RE, Swinkels DW, (2011) Hepcidin in human iron disorders: diagnostic implications. Clin Chem 257: 1650-1669.
2. Park CH, Valore EV, Waring AJ, Ganz T, (2001) Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J Biol Chem 276: 7806-7810.
3. Kemna E, Tjalsma H, Laarakkers C, Nemeth E, Willems H, et al. (2005) Novel urine hepcidin assay by mass spectrometry. Blood 106: 3268-3270.
4. Kemna EH, Tjalsma H, Podust VN, Swinkels DW, (2007) Mass spectrometry-based hepcidin measurements in serum and urine: analytical aspects and clinical implications. Clin Chem 53: 620-628.
5. Swinkels DW, Girelli D, Laarakkers C, Kroot J, Campostrini N, et al. (2008) Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry. PLoS ONE 3: e2706.
6. Kroot JJC, Laarakkers CM, Geurts-Moespot AJ, Grebenchtchikov N, Pickkers P, et al. (2010) Immunochemical and Mass-Spectrometry-Based Serum Hepcidin Assays for Iron Metabolism Disorders. Clin Chem 256: 1570-1579.
7. Campostrini N, Traglia M, Martinelli N, Corbella M, Cocca M, et al. (2012) Serum levels of the hepcidin-20 isoform in a large general population: The Val Borbera study. J Proteomics 76: 28-35.
8. Tomosugi N, Kawabata H, Wakatabe R, Higuchi M, Yamaya H, et al. (2006) Detection of serum hepcidin in renal failure and inflammation by using ProteinChip System. Blood 108: 1381-1387.
9. Peters HP, Laarakkers CM, Swinkels DW, Wetzels JF, (2010) Serum hepcidin-25 levels in patients with chronic kidney disease are independent of glomerular filtration rate. Nephrol Dial Transplant 25: 848-853.
10. Campostrini N, Castagna A, Zaninotto F, Bedogna V, Tessitore N, et al. (2010) Evaluation of hepcidin isoforms in hemodialysis patients by a proteomic approach based on SELDI-TOF MS. J Biomed Biotechnol 2010: 329646.
11. Schranz M, Bakry R, Creus M, Bonn G, Vogel W, et al. (2009) Activation and inactivation of the iron hormone hepcidin: Biochemical characterization of prohepcidin cleavage and sequential degradation to N-terminally truncated hepcidin isoforms. Blood Cells Mol Dis 43: 169-179.
12. Valore EV, Ganz T, (2008) Posttranslational processing of hepcidin in human hepatocytes is mediated by the prohormone convertase furin. Blood Cells Mol Dis 40: 132-138.
13. van Santen S, van Dongen-Lases EC, de Vegt F, Laarakkers CM, van Riel PL, et al. (2011) Hepcidin and hemoglobin content parameters in the diagnosis of iron deficiency in rheumatoid arthritis patients with anemia. Arthritis Rheum. 63: 3672-3680.
14. Santini V, Girelli D, Sanna A, Martinelli N, Duca L, et al. (2011) Hepcidin levels and their determinants in different types of myelodysplastic syndromes. PLoS One 6: e23109.
15. Nai A, Pagani A, Silvestri L, Campostrini N, Corbella M, et al. (2011) TMPRSS6 rs855791 modulates hepcidin transcription in vitro and serum hepcidin levels in normal individuals. Blood 118: 4459-4462.
16. Peters HP, Rumjon A, Bansal SS, Laarakkers CM, van den Brand JA, et al. (2012) Intra-individual variability of serum hepcidin-25 in haemodialysis patients using mass spectrometry and ELISA. Nephrol Dial Transplant 27: 3923-3929.
17. Ambaglio I, Malcovati L, Papaemmanuil E, Laarakkers CM, Della Porta MG, et al. (2013) Inappropriately low hepcidin levels in patients with myelodysplastic syndrome carrying a somatic mutation of SF3B1. Haematologica 98: 420-342.
18. Schaap CC, Hendriks JC, Kortman GA, Klaver SM, Kroot JJ, et al. (2013) Diurnal rhythm rather than dietary iron mediates daily hepcidin variations. Clin Chem 59: 527-535.
19. Ward DG, Roberts K, Stonelake P, Goon P, Zampronio CG, et al. (2008) SELDI-TOF-MS determination of hepcidin in clinical samples using stable isotope labelled hepcidin as an internal standard. Proteome Sci 6: 28.
20. Murao N, Ishigai M, Yasuno H, Shimonaka Y, Aso Y, (2007) Simple and sensitive quantification of bioactive peptides in biological matrices using liquid chromatography/selected reaction monitoring mass spectrometry coupled with trichloroacetic acid clean-up. Rapid Commun Mass Spectrom 21: 4033-4038.
21. Kobold U, Dulffer T, Dangl M, Escherich A, Kubbies M, et al. (2008) Quantification of hepcidin-25 in human serum by isotope dilution micro-HPLC-tandem mass spectrometry. Clin Chem 54: 1584-1586.
22. Li H, Rose MJ, Tran L, Zhang J, Miranda LP, et al. (2009) Development of a method for the sensitive and quantitative determination of hepcidin in human serum using LC-MS/MS. J Pharmacol Toxicol Methods 59: 171-180.
23. Bansal SS, Abbate V, Bomford A, Halket JM, Macdougall IC, et al. (2010) Quantitation of hepcidin in serum using ultra-high-pressure liquid chromatography and a linear ion trap mass spectrometer. Rapid Commun Mass Spectrom 24: 1251-1259.
24. Anderson DS, Kirchner M, Kellogg M, Kalish LA, Jeong JY, et al. (2011) Design and validation of a high-throughput matrix-assisted laser desorption ionization time-of-flight mass spectrometry method for quantification of hepcidin in human plasma. Anal Chem 83: 8357-8362.
25. Kroot JJ, van Herwaarden AE, Tjalsma H, Jansen RT, Hendriks JC, et al. (2012) Second round robin for plasma hepcidin methods: first steps toward harmonization. Am J Hematol 87: 977-983.
26. Kroot JJ, Kemna EH, Bansal SS, Busbridge M, Campostrini N, et al. (2009) Results of the first international round robin for the quantification of urinary and plasma hepcidin assays: need for standardization. Haematologica. 94: 1748-1752.
27. Hunter HN, Fulton DB, Ganz T, Vogel HJ, (2002) The solution structure of human hepcidin, a peptide hormone with antimicrobial activity that is involved in iro uptake and hereditary hemochromatosis. J Biol Chem 277: 37597-37603.
28. van Dijk BA, Kemna EH, Tjalsma H, Klaver SM, Wiegerinck ET, et al. (2007) Effect of the new HJV-L165X mutation on penetrance of HFE. Blood 109: 5525-5526.
29. Hohlbaum AM, Trentman S, Gille H, Allersdorfer A, Belaiba RS, et al. (2011) Discovery and Preclinical Characterization of a Novel Hepcidin Antagonist with Tunable PK/PD Properties for the Treatment of Anemia in Different Patient Populations Blood 118: Abstract 687.
30. Schönfeld D, Matschiner G, Chatwell L, Trentmann S, Gille H, et al. (2009) An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies. Proc Natl Acad Sci USA 106: 8198-8203.
31. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T, (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci 4: 2411-2423.
32. Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, et al. (2004) Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 306: 2090-2093.
33. Nemeth E, Preza GC, Jung CL, Kaplan J, Waring AJ, et al. (2006) The N-terminus of hepcidin is essential for its interaction with ferroportin: structure-function study. Blood 107: 328-333.
34. Rivera S, Nemeth E, Gabayan V, Lopez MA, Farshidi D, et al. (2005) Synthetic hepcidin causes rapid dose-dependent hypoferremia and is concentrated in ferroportin-containing organs. Blood 106: 2196-2199.
35. Itkonen O, Parkkinen J, Stenman UH, Hämäläinen E, (2012) Preanalytical factors and reference intervals for serum hepcidin LC-MS/MS method. Clin Chim Acta 413: 696-701.
36. Kroot JJ, Hendriks JC, Laarakkers CM, Klaver SM, Kemna EH, et al. (2009) (Pre)analytical imprecision, between-subject variability, and daily variations in serum and urine hepcidin: implications for clinical studies. Anal Biochem 389: 124-129.
37. Ganz T, Olbina G, Girelli D, Nemeth E, Westerman M, (2008) Immunoassay for human serum hepcidin. Blood 112: 4292-4297.