Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Dvorak, Pavel ^a,b   Chrast, Lukas ^a,b   Nikel, Pablo I ^c   Fedr, Radek ^b,d   Soucek, Karel ^a,b,d   Sedlackova, Miroslava ^a   Chaloupkova, Radka ^a,b   Lorenzo, Víctor ^c   Prokop, Zbynek ^a,b   Damborsky, Jiri ^a,b  

^a MASARYK UNIVERSITY   (Czech Republic)

^b ST ANNE S UNIVERSITY HOSPITAL   (Czech Republic)

^c CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^d INSTITUTE OF BIOPHYSICS   (Czech Republic)

Author keywords

1,2,3 trichloropropane; Escherichia coli; Heterologous metabolic pathway; Isopropyl D 1 thiogalactopyranoside; Lactose; Metabolic burden; Metabolic engineering; Substrate toxicity

Indexed keywords

1,2,3 TRICHLOROPROPANE; CHEMICAL COMPOUND; ISOPROPYL THIOGALACTOSIDE; LACTOSE; SUGAR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BIODEGRADATION; CONTROLLED STUDY; ELECTRON MICROSCOPY; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; HETEROLOGOUS EXPRESSION; HOST; NONHUMAN; PHYSIOLOGICAL STRESS; PLASMID; POLLUTANT; GENETICS; METABOLIC ENGINEERING; METABOLISM;

ESCHERICHIA COLI; ISOPROPYL THIOGALACTOSIDE; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS;

EID: 84954144197     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0393-3     Document Type: Article

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