Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress

Applied and Environmental Microbiology

Volumn 68, Issue 11, 2002, Pages 5209-5216

  Amor, Kaouther Ben ^a   Breeuwer, Pieter ^a   Verbaarschot, Patrick ^a   Rombouts, Frank M ^a   Akkermans, Antoon D L ^a   De Vos, Willem M ^a   Abee, Tjakko ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; CELL CULTURE; CELLS; ENZYMES; FLOW OF FLUIDS; SALTS;

BILE SALT STRESSES;

BACTERIA;

BILE SALT; CARBOXYFLUORESCEIN; ESTERASE; OXONIC ACID; PROBIOTIC AGENT; PROPIDIUM IODIDE;

FLOW CYTOMETRY;

ARTICLE; BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM ANIMALIS; CELL DAMAGE; CELL DEATH; CELL MEMBRANE PERMEABILITY; CELL MEMBRANE POTENTIAL; CELL SELECTION; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUORESCENCE; NONHUMAN; PLATE COUNT; SALT STRESS;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM ANIMALIS; UNCULTURED ACTINOMYCETE;

EID: 0036841272     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.11.5209-5216.2002     Document Type: Article

References (38)

1. Barer, M. R., and C. R. Harwood. 1999. Bacterial viability, and culturability. Adv. Microb. Physiol. 41:93-137.
2. Bernstein, C., H. Bernstein, C. M. Payen, S. E. Beard, and J. Schneider. 1999. Bile salt activation of stress response promoters in Escherichia coli. Curr. Microbiol. 39:68-72.
3. Breeuwer, P., and T. Abee. 2000. Assessment of viability of microorganisms employing fluorescence techniques. Int. J. Food Microbiol. 55:193-200.
4. Bunthof, C. J., S. van Schalkwijk, W. Meijer, T. Abee, and J. Hugenholtz. 2001. Fluorescent method for monitoring cheese starter permeabilization and lysis. Appl. Environ. Microbiol. 67:4264-4271.
5. Bunthof, C. J., K. Bloemen, P. Breeuwer, F. M. Rombouts, and T. Abee. 2001. Flow cytometry assessment of the viability of lactic acid bacteria. Appl. Environ. Microbiol. 67:2326-2335.
6. Davey, H. M., D. H. Welchart, D. B. Douglas, and A. S. Kaprelyants. 1999. Estimation of microbial viability using flow cytometry, p. 11.3.11-11.3.20. In P. Robinson, Z. Darzynkiewics, P. Dean, A. Orfao, P. Rabinovitch, H. Tank, and L. Wheeless (ed.), Current Protocols in Cytometry, Wiley, New York, N.Y.
7. Davey, H. M., and D. B. Kell. 1996. Flow cytometry and cell sorting of heterogeneous microbial populations: The importance of single-cell analyses. Microbiol. Rev. 60:641-696.
8. Diaper J. P., and C. Edwards. 1994. The use of fluorogenic esters to detect viable bacteria by flow cytometry. J. Appl. Bacteriol. 77:221-228.
9. Ericsson, M., D. Hanstorp. P. Hagberg, J. Enger, and T. Nyström. 2000. Sorting out bacterial viability with optical tweezers. J. Bacteriol. 182:5551-5555.
10. Hewitt, C. J., and G. Nebe-von-Caron. 2001. An industrial application of multiparameter flow cytometry: Assessment of cell physiology state and its application to the study of microbial fermentations. Cytometry. 44:179-187.
11. Hewitt, C. J., G. Nebe-von-Caron, A. W. Niennow, and C. M. McFarlane. 1999. The use of multi-parameter flow cytometry to compare the physiological response of Escherichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivations. J. Biotechnol. 75:251-264.
12. Humphreys, M. J., R. Allman, and D. Lloyd. 1994. Determination of the viability of Trichomonas vaginalis using flow cytometry. Cytometry. 15:343-348.
13. Jepras, R. I., F. E. Paul, S. C. Pearson, and M. J. Wilkinson. 1997. Rapid assessment of antibiotic effects of Escherichia coli by bis-(1,3-dibutylbarbituric acid) trimethine oxonol and flow cytometry. Antimicrob. Agents. Chemother. 41:2001-2005.
14. Joux, F., and P. Lebaron. 2000. Use of fluorescent probes to assess physiological functions of bacteria at single-cell level. Microbes. Infect. 2:1523-1535.
15. Kaprelyants, A. S., G. V. Mukamolova, H. M. Davey, and D. B. Kell. 1996. Quantitative analysis of the physiological heterogeneity within starved cultures of Micrococcus luteus by flow cytometry and cell sorting. Appl. Environ. Microbiol. 62:1311-1316.
16. Kell, D. B., A. S. Kaprelyants, D. H. Welchart, C. R. Harwood, and M. R. Barer. 1998. Viability and activity in readily culturable bacteria: A review and discussion of practical issues. Antonie Leeuwenhoek 73:169-187.
17. Klaenhammer, T., R., and M. J. Kullen. 1999. Selection and design of probiotics. Int. J. Food. Microbiol. 50:45-57.
18. Lopez-Amoros, R., J. Comas, and J. Vives-Rego. 1995. Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol. Appl. Environ. Microbiol. 61:2521-2526.
19. 4(3), propidium iodide, and CTC. Cytometry. 29:298-305.
20. Mason, D. J., R. Lopez-Amoros, R. Allman, J. M. Stark, and D. Lloyd. 1995. The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria. J. Appl. Bacteriol. 78:309-315.
21. Mason, D. J., R. Allman, J. M. Stark, and D. Lloyd. 1994. Rapid estimation of bacterial antibiotic susceptibility with flow cytometry. J. Microsc. 176:8-16.
22. Meile, L., W. Ludwig, U. Rueger, C. Gut, P. Kaufmann, G. Dasen, S. Wenger, and M. Teuber. 1997. Bifidobacterium lactis sp. nov., a moderately oxygen tolerant species isolated from fermented milk. Syst. Appl. Microbiol. 20:56-64.
23. Nebe-von-Caron, G., P. J. Stephens, C. H. Hewitt, J. R. Powell, and R. A. Badley. 2000. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J. Microbiol. Methods. 42:97-114.
24. Novo, D., N. G. Perlmutter, R. H. Hunt, and H. M. Shapiro. 1999. Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and ratiometric technique. Cytometry. 35:55-63.
25. Novo, D. J., N. G. Perlmutter, R. H. Hunt, and H. M. Shapiro. 2000. Multiparameter flow cytometric analysis of antibiotic effects on membrane potential, membrane permeability, and bacterial counts of Staphylococcus aureus and Micrococcus luteus. Antimicrob. Agents. Chemother. 44:827-834.
26. Ouwehand, A. C., P. V. Kirjavainen, C. Shortt, and S. Salminen. 1999. Probiotics: Mechanisms and established effects. Int. Dairy. J. 9:43-52.
27. Porter, J., C. Edwards, and R. W. Pickup. 1995. Rapid assessment of physiological status in Escherichia coli using fluorescent probes. J. Appl. Microbiol. 79:399-408.
28. Porter, J., D. Deere, R. Pickup, and C. Edwards. 1996. Fluorescent probes and flow cytometry: New insights into environmental bacteriology. Cytometry. 23:91-96.
29. Ritz, M., J. L. Tholozan, M. Fedeeighi, and M. F. Pilet. 2001. Morphological and physiological characterization of Listeria monocytogenes subjected to high hydrostatic pressure. Appl. Environ. Microbiol. 67:2240-2247.
30. Schmidt, G., and R. Zink. 2000. Basic features of the stress response in three species of bifidobacteria: B. longum, B. adolescentis, and B. breve. Int. J. Food. Microbiol. 55:41-45.
31. Shapiro, H. M. 2000. Microbial analysis at the single-cell level: Tasks and techniques. J. Microbiol. Methods 42:3-16.
32. Shapiro, H. M. 1995. Practical Flow Cytometry, 3rd Ed. Alan R. Liss, Inc. New York, N.Y.
33. Shimamura, S., F. Abe, N. Ishibashi, H. Miyakawa, T. Yaeshima, T. Araya, and M. Tomita. 1992. Relationship between oxygen sensitivity and oxygen metabolism of bifidobacterium species. J. Dairy. Sci. 75:3296-3306.
34. Sincock, S. A., and J. P. Robinson. 2001. Flow cytometric analysis of microorganisms. Methods. Cell. Biol. 64:511-537.
35. Suller, M. T. E., and D. Lloyd. 1999. Fluorescence monitoring of antibiotic-induced bacterial damage using flow cytometry. Cytometry 35:235-241.
36. Tannock, G. W. 1999. Microecology of Lactobacilli and Bifidobacteria inhabiting the digestive tract: Essential knowledge for successful probiotic research, p 17-31. In L. A. Hanson and R. H. Yolken (ed.), Probiotics, Other Nutritional Factors, and Intestinal Microflora. Nestle Nutrition Services, Lippincott-Raven, Philadelphia, Pa.
37. Wallner, G., B. Fuchs, S. Spring, W. Beisker, and R. Amann. 1997. Flow sorting of microorganisms for molecular analysis. Appl. Environ. Microbiol. 63:4223-4231.
38. Ward, D., R. Weller, and M. M. Bateson. 1990. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature. 345:63-65.