Evaluation of three industrial Escherichia coli strains in fed-batch cultivations during high-level SOD protein production

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Marisch, Karoline ^a,b   Bayer, Karl ^a   Cserjan Puschmann, Monika ^a,b   Luchner, Markus ^a,b   Striedner, Gerald ^a,b  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

Bioreactor cultivation; E. coli; Fed batch; Recombinant protein production; SOD; Strain characterization

Indexed keywords

CARBON; ESCHERICHIA COLI PROTEIN; RECOMBINANT PROTEIN; SUPEROXIDE DISMUTASE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BIOMASS; CELL VIABILITY; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; FED BATCH CULTURE; FLOW CYTOMETRY; GENOTYPE; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

BATCH CELL CULTURE TECHNIQUES; CARBON; ESCHERICHIA COLI; GENE DOSAGE; GENETIC VECTORS; PLASMIDS; RECOMBINANT PROTEINS; SOLUBILITY; SUPEROXIDE DISMUTASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84878717020     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-58     Document Type: Article

References (35)

1. Ferrer-Miralles N, Domingo-Espin J, Corchero JL, Vazquez E, Villaverde A. Microbial factories for recombinant pharmaceuticals. Microb Cell Fact 2009, 8:17. 10.1186/1475-2859-8-17, 2669800, 19317892.
2. Jeong H, Barbe V, Lee CH, Vallenet D, Yu DS, Choi S-H, Couloux A, Lee S-W, Yoon SH, Cattolico L, et al. Genome sequences of Escherichia coli B strains REL606 and BL21(DE3). J Mol Biol 2009, 394:644-652. 10.1016/j.jmb.2009.09.052, 19786035.
3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T. Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol 2006, 2:2006 0007. 1681481, 16738553.
4. Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, et al. The complete genome sequence of Escherichia coli K-12. Science 1997, 277:1453-1462. 10.1126/science.277.5331.1453, 9278503.
5. Sørensen HP, Mortensen KK. Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 2005, 115:113-128. 10.1016/j.jbiotec.2004.08.004, 15607230.
6. Baneyx F. Recombinant protein expression in Escherichia coli. Curr Opin Biotechnol 1999, 10:411-421. 10.1016/S0958-1669(99)00003-8, 10508629.
7. Makrides SC. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol Rev 1996, 60:512-538. 239455, 8840785.
8. Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 1986, 189:113-130. 10.1016/0022-2836(86)90385-2, 3537305.
9. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 1990, 185:60-89.
10. Bentley WE, Mirjalili N, Andersen DC, Davis RH, Kompala DS. Plasmid-encoded protein: the principal factor in the " metabolic burden" associated with recombinant bacteria. Biotechnol Bioeng 1990, 35:668-681. 10.1002/bit.260350704, 18592563.
11. Carneiro S, Ferreira EC, Rocha I. Metabolic responses to recombinant bioprocesses in Escherichia coli. J Biotechnol 2013, 164:396-408. 10.1016/j.jbiotec.2012.08.026, 23022453.
12. Hoffmann F, Rinas U. Stress induced by recombinant protein production in Escherichia coli. Adv Biochem Eng Biotechnol 2004, 89:73-92.
13. Baneyx F, Mujacic M. Recombinant protein folding and misfolding in Escherichia coli. Nat Biotech 2004, 22:1399-1408.
14. Fahnert B, Lilie H, Neubauer P. Inclusion bodies: formation and utilisation. Adv Biochem Eng Biotechnol 2004, 89:93-142.
15. Striedner G, Pfaffenzeller I, Luchner M, Nemecek S, Grabherr R, Bayer K. Plasmid-free T7-based Escherichia coli expression systems. Biotechnol Bioeng 2009, 105:786-794.
16. Noronha SB, Yeh HJC, Spande TF, Shiloach J. Investigation of the TCA cycle and the glyoxylate shunt in Escherichia coli BL21 and JM109 using 13C-NMR/MS. Biotechnol Bioeng 2000, 68:316-327. 10.1002/(SICI)1097-0290(20000505)68:3<316::AID-BIT10>3.0.CO;2-2, 10745200.
17. Shiloach J, Kaufman J, Guillard AS, Fass R. Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (DE3) and Escherichia coli JM109. Biotechnol Bioeng 1996, 49:421-428.
18. Shiloach J, Reshamwala S, Noronha SB, Negrete A. Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends - example E. coli. Curr Opin Biotechnol 2010, 21:21-26. 10.1016/j.copbio.2010.01.001, 20116994.
19. Luli GW, Strohl WR. Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl Environ Microbiol 1990, 56:1004-1011. 184335, 2187400.
20. Kramer W, Elmecker G, Weik R, Mattanovich D, Bayer K. Kinetic studies for the optimization of recombinant protein formation. Ann N Y Acad Sci 1996, 782:323-333. 10.1111/j.1749-6632.1996.tb40572.x, 8659908.
21. Choi JH, Keum KC, Lee SY. Production of recombinant proteins by high cell density culture of Escherichia coli. Chem Eng Sci 2006, 61:876-885.
22. Bauer S, Ziv E. Dense growth of aerobic bacteria in a bench-scale fermentor. Biotechnol Bioeng 1976, 18:81-94. 10.1002/bit.260180107, 813791.
23. Roels JA. Application of macroscopic principles to microbial metabolism. Biotechnol Bioeng 1980, 22:2457-2514.
24. Nemecek S, Marisch K, Juric R, Bayer K. Design of transcriptional fusions of stress sensitive promoters and GFP to monitor the overburden of Escherichia coli hosts during recombinant protein production. Bioprocess Biosystems Eng 2008, 31:47-53.
25. Cserjan-Puschmann M, Kramer W, Duerrschmid E, Striedner G, Bayer K. Metabolic approaches for the optimisation of recombinant fermentation processes. Appl Microbiol Biotechnol 1999, 53:43-50. 10.1007/s002530051612, 10645624.
26. Magnusson LU, Farewell A, Nystroem T. ppGpp: a global regulator in Escherichia coli. Trends Microbiol 2005, 13:236-242. 10.1016/j.tim.2005.03.008, 15866041.
27. Andersson L, Yang S, Neubauer P, Enfors S-O. Impact of plasmid presence and induction on cellular responses in fed batch cultures of Escherichia coli. J Biotechnol 1996, 46:255-263. 10.1016/0168-1656(96)00004-1, 8672292.
28. Summers DK, Beton CW, Withers HL. Multicopy plasmid instability: the dimer catastrophe hypothesis. Mol Microbiol 1993, 8:1031-1038. 10.1111/j.1365-2958.1993.tb01648.x, 8361350.
29. Heel T, Paal M, Schneider R, Auer B. Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion - tag. Microb Cell Fact 2010, 9:92. 10.1186/1475-2859-9-92, 3001420, 21092285.
30. Striedner G, Cserjan-Puschmann M, Potschacher F, Bayer K. Tuning the transcription rate of recombinant protein in strong Escherichia coli expression systems through repressor titration. Biotechnol Prog 2003, 19:1427-1432. 10.1021/bp034050u, 14524702.
31. Hewitt CJ, Nebe-Von Caron G, Nienow AW, McFarlane CM. Use of multi-staining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures. Biotechnol Bioeng 1999, 63:705-711. 10.1002/(SICI)1097-0290(19990620)63:6<705::AID-BIT8>3.0.CO;2-M, 10397827.
32. Porstmann T, Wietschke R, Schmechta H, Grunow R, Porstmann B, Bleiber R, Pergande M, Stachat S, Von Baehr R. A rapid and sensitive enzyme immunoassay for Cu/Zn superoxide dismutase with polyclonal and monoclonal antibodies. Clin Chim Acta 1988, 171:1-10. 10.1016/0009-8981(88)90285-9, 2964951.
33. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685. 10.1038/227680a0, 5432063.
34. Clementschitsch F, Kern J, Poettschacher F, Bayer K. Sensor combination and chemometric modelling for improved process monitoring in recombinant E. coli fed-batch cultivations. J Biotechnol 2005, 120:183-196. 10.1016/j.jbiotec.2005.05.030, 16139381.
35. Luchner M, Gutmann R, Bayer K, Dunkl J, Hansel A, Herbig J, Singer W, Strobl F, Winkler K, Striedner G. Implementation of proton transfer reaction-mass spectrometry (PTR-MS) for advanced bioprocess monitoring. Biotechnol Bioeng 2012, 109:3059-3069. 10.1002/bit.24579, 22711525.