Effect of substrate and IPTG concentrations on the burden to growth of Escherichia coli on glycerol due to the expression of Lac proteins

Applied Microbiology and Biotechnology

Volumn 93, Issue 6, 2012, Pages 2543-2549

  Malakar, Pushkar ^a   Venkatesh, K V ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY BOMBAY   (India)

Author keywords

Burden on growth; Cost due to protein expression; Early exponential phase; Escherichia coli; Glycerol; IPTG; Late exponential phase

Indexed keywords

BIOTECHNOLOGY INDUSTRY; CARBON SOURCE; CELLULAR RESOURCES; COST-INCREASES; EARLY-EXPONENTIAL PHASE; GLYCEROL CONCENTRATION; HILL EQUATION; IPTG; LATE-EXPONENTIAL PHASE; PROTEIN EXPRESSIONS;

COSTS; ESCHERICHIA COLI; PROTEINS; SUGARS;

GLYCEROL;

BETA GALACTOSIDASE; GLYCEROL; ISOPROPYL THIOGALACTOSIDE; LACTOSE;

ALCOHOL; BIOTECHNOLOGY; CONCENTRATION (COMPOSITION); FECAL COLIFORM; GENE EXPRESSION; GROWTH RATE; PROTEIN; SUBSTRATE;

ARTICLE; BACTERIAL GROWTH; CARBON SOURCE; CELL SUSPENSION; COST; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GROWTH PERIOD; GROWTH RATE; NONHUMAN; PROTEIN EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; LACTOSE OPERON; METABOLISM;

ESCHERICHIA COLI;

ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GLYCEROL; ISOPROPYL THIOGALACTOSIDE; LAC OPERON; LACTOSE;

EID: 84862279138     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3642-3     Document Type: Article

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