Biosynthetic engineering and fermentation media development leads to gram-scale production of spliceostatin natural products in Burkholderia sp.

Metabolic Engineering

Volumn 33, Issue , 2016, Pages 67-75

  Eustáquio, Alessandra S ^a,b   Chang, Li Ping ^a   Steele, Greg L ^a   O'Donnell, Christopher J ^a   Koehn, Frank E ^a  

^a PFIZER INC   (United States)

^b UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Antibody drug conjugate; Biosynthesis; Polyketide; Spliceostatin; Splicing inhibitor; Thailanstatin

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; BOTTLES; CHEMOTHERAPY; FERMENTATION; GENE EXPRESSION; GENES; KETONES; MOLECULES;

ANTIBODY-DRUG CONJUGATES; BIOSYNTHETIC ENGINEERINGS; CYTOCHROME P450 GENES; DEVELOPMENT APPROACH; POLYKETIDES; PRECLINICAL DEVELOPMENT; SPLICEOSTATIN; THAILANSTATIN;

DRUG PRODUCTS;

2 OXOGLUTARIC ACID; ARABINOSE; CARBOXYLIC ACID; CYTOCHROME P450; DIOXYGENASE; DISSOLVED OXYGEN; IRON; NATURAL PRODUCT; NITROGEN; SPLICEOSTATIN; THAILANSTATIN A; UNCLASSIFIED DRUG; ANTINEOPLASTIC AGENT; BACTERIAL PROTEIN; CULTURE MEDIUM; PYRAN DERIVATIVE; SPIRO COMPOUND;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM MUTANT; BIOREACTOR; BIOSYNTHESIS; BIOTECHNOLOGICAL PRODUCTION; BURKHOLDERIA; CARBON SOURCE; FERMENTATION; METABOLIC ENGINEERING; NONHUMAN; PRECURSOR; PRIORITY JOURNAL; PROMOTER REGION; WILD TYPE; CHEMISTRY; CULTURE MEDIUM; GENETIC ENHANCEMENT; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; PROCEDURES;

ANTINEOPLASTIC AGENTS; BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; BURKHOLDERIA; CULTURE MEDIA; GENETIC ENHANCEMENT; METABOLIC ENGINEERING; PYRANS; SPIRO COMPOUNDS;

EID: 84949035742     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.11.003     Document Type: Article

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