The development of an efficient system for heterologous expression of cytochrome P450s in Escherichia coli using hemA gene co-expression

Protein Expression and Purification

Volumn 46, Issue 1, 2006, Pages 47-55

  Harnastai, Ivan N ^a   Gilep, Andrei A ^a   Usanov, Sergey A ^a  

^a INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

Author keywords

5 Aminolevulinic acid; Cytochrome P450; Glutamyl tRNA reductase; hemA; Heme biosynthesis; Hemeprotein

Indexed keywords

ALDEHYDE DEHYDROGENASE; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; GLUTAMYL TRNA REDUCTASE; HEME; HYBRID PROTEIN; STEROID 17ALPHA MONOOXYGENASE; STEROID 21 MONOOXYGENASE;

ARTICLE; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; KINETICS; METABOLISM; MITOCHONDRION; MOLECULAR CLONING; MUTAGENESIS; RESTRICTION MAPPING;

ALDEHYDE OXIDOREDUCTASES; CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME; CLONING, MOLECULAR; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 CYP1A2; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; GENETIC VECTORS; HEME; KINETICS; MITOCHONDRIA; MUTAGENESIS; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; STEROID 17-ALPHA-HYDROXYLASE; STEROID 21-HYDROXYLASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 32644463149     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.006     Document Type: Article

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