Evidence That Compound i Is the Active Species in Both the Hydroxylase and Lyase Steps by Which P450scc Converts Cholesterol to Pregnenolone: EPR/ENDOR/Cryoreduction/Annealing Studies

Biochemistry

Volumn 54, Issue 48, 2015, Pages 7089-7097

  Davydov, Roman ^a   Strushkevich, Natallia ^b   Smil, David ^c   Yantsevich, Aliaksei ^b   Gilep, Andrey ^b   Usanov, Sergey ^b   Hoffman, Brian M ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ANNEALING; CARRIER MOBILITY; CHOLESTEROL; COORDINATION REACTIONS; HORMONES; HYDROXYLATION; IRON; ISOTOPES; MAGNETIC RESONANCE; PARAMAGNETIC RESONANCE; STEROID HORMONES;

ACTIVE INTERMEDIATES; CYTOCHROME P450SCC; DOUBLE RESONANCE; ELECTRON PARAMAGNETIC RESONANCES (EPR); ENZYMATIC PROCESS; FERRIC HYDROPEROXO; PRIMARY PRODUCTS; SOLVENT KINETIC ISOTOPE EFFECTS;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

CHOLESTEROL; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); ISOTOPE; LYASE; OXYGENASE; PREGNENOLONE; SOLVENT; 22-HYDROXYCHOLESTEROL; CHOLESTEROL DERIVATIVE;

ARTICLE; ELECTRON NUCLEAR DOUBLE RESONANCE; ELECTRON SPIN RESONANCE; HYDROXYLATION; MEASUREMENT; OXYGENATION; PRIORITY JOURNAL; ANIMAL; BOVINE; CHEMISTRY; HUMAN; METABOLISM; OXIDATION REDUCTION REACTION;

ANIMALS; CATTLE; CHOLESTEROL; CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME; ELECTRON SPIN RESONANCE SPECTROSCOPY; HUMANS; HYDROXYCHOLESTEROLS; HYDROXYLATION; OXIDATION-REDUCTION; PREGNENOLONE;

EID: 84948983123     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.5b00903     Document Type: Article

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