Trigonella foenum (Fenugreek) induced apoptosis in hepatocellular carcinoma cell line, HepG2, mediated by upregulation of p53 and proliferating cell nuclear antigen

BioMed Research International

Volumn 2015, Issue , 2015, Pages

  Khalil, Mahmoud I M ^a   Ibrahim, Mohamed M ^a,b   El Gaaly, Gehan A ^b   Sultan, Ahmed S ^a  

^a Alexandria University Faculty Of Science   (Egypt)

^b KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; CYCLINE; FENUGREEK EXTRACT; METHANOL; NARINGENIN; PROTEIN BAX; PROTEIN P53; SQUALENE; PLANT EXTRACT;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER INHIBITION; CELL STRUCTURE; CONTROLLED STUDY; DRUG CYTOTOXICITY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HEPG2 CELL LINE; HUMAN; HUMAN CELL; MASS FRAGMENTOGRAPHY; MTT ASSAY; NONHUMAN; PLANT SEED; PROTEIN EXPRESSION; THERAPY EFFECT; TRIGONELLA; TRIGONELLA FOENUM; UPREGULATION; CELL SURVIVAL; CHEMISTRY; DRUG EFFECTS; HEP-G2 CELL LINE; METABOLISM;

APOPTOSIS; CELL SURVIVAL; HEP G2 CELLS; HUMANS; PLANT EXTRACTS; PROLIFERATING CELL NUCLEAR ANTIGEN; TRIGONELLA; TUMOR SUPPRESSOR PROTEIN P53; UP-REGULATION;

EID: 84947093093     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2015/914645     Document Type: Article

References (68)

1. J. Ferlay, I. Soerjomataram, R. Dikshit et al., "Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012, " International Journal of Cancer, vol. 136, no. 5, pp. E359-E386, 2015
2. K. A. McGlynn, L. Tsao, A. W. Hsing, S. S. Devesa, and J. F. Fraumeni Jr., "International trends and patterns of primary liver cancer, " International Journal of Cancer, vol. 94, no. 2, pp. 290-296, 2001
3. M. K. Shaker, H. M. Abdella, M. O. Khalifa, and A. K. E. Dorry, "Epidemiological characteristics of hepatocellular carcinoma in Egypt: a retrospective analysis of 1313 cases, " Liver International, vol. 33, no. 10, pp. 1601-1606, 2013
4. N. Abdel-Hamid, "Update to risk factors ofHCC, " International Journal ofMedicine andMedical Sciences, vol. 1, no. 3, pp. 38-43, 2009
5. A. S. Sultan, J. Xie, M. J. LeBaron, E. L. Ealley, M. T. Nevalainen, and H. Rui, "Stat5 promotes homotypic adhesion and inhibits invasive characteristics of human breast cancer cells, " Oncogene, vol. 24, no. 5, pp. 746-760, 2005
6. A. S. Sultan, H. Brim, and Z. A. Sherif, "Co-overexpression of Janus kinase 2 and signal transducer and activator of transcription 5a promotes differentiation of mammary cancer cells through reversal of epithelial-mesenchymal transition, " Cancer Science, vol. 99, no. 2, pp. 272-279, 2008
7. J. Sun and R. Hai Liu, "Cranberry phytochemical extracts induce cell cycle arrest and apoptosis in human MCF-7 breast cancer cells, " Cancer Letters, vol. 241, no. 1, pp. 124-134, 2006
8. A. S. Sultan and Z. A. Sherif, "Abstract B42: Jak2/Stat5a overexpression synergizes with mistletoe (Viscum album) extracts, and/or diallyl trisulfide (DATS) to inhibit breast cancer stemcell characteristics, epithelial-mesenchymal transition, and invasion potential, " Molecular Cancer Therapeutics, vol. 10, supplement 11, p. B42, 2011
9. K. H. Kwon, A. Barve, S. Yu, M.-T. Huang, and A.-N. T. Kong, "Cancer chemoprevention by phytochemicals: potential molecular targets, biomarkers and animal models, " Acta Pharmacologica Sinica, vol. 28, no. 9, pp. 1409-1421, 2007
10. O. R. Omobuwajo, A. Abdu, O. A. Igbeneghu, I. O. Agboola, and G. O. Alade, "Preliminary investigation of herbal soap incorporating Cassia senna (L) Roxb leaves and Ageratum conyzoides linn whole plant powders, " Continental Journal of Pharmaceutical Sciences, vol. 5, pp. 1-10, 2011
11. C. W. Fetrow and J. R. Avila, Professional?s Handbook of Complementary & Alternative Medicines, Springhouse Corporation, SpringHouse, Pa, USA, 1999
12. R.Mebazaa, A.Mahmoudi, M. Fouchet et al., "Characterisation of volatile compounds in Tunisian fenugreek seeds, " Food Chemistry, vol. 115, no. 4, pp. 1326-1336, 2009
13. M. M. Naidu, B. N. Shyamala, J. PuraNaik, G. Sulochanamma, and P. Srinivas, "Chemical composition and antioxidant activity of the husk and endosperm of fenugreek seeds, " LWT-Food Science and Technology, vol. 44, no. 2, pp. 451-456, 2011
14. W. G. Taylor, M. S. Zaman, Z. Mir et al., "Analysis of steroidal sapogenins from Amber Fenugreek (Trigonella foenumgraecum) by capillary gas chromatography and combined gas chromatography/mass spectrometry, " Journal of Agricultural and Food Chemistry, vol. 45, no. 3, pp. 753-759, 1997
15. Y.Wu, W. Cui, N. A. M. Eskin, andH.D. Goff, "An investigation of four commercial galactomannans on their emulsion and rheological properties, " Food Research International, vol. 42, no. 8, pp. 1141-1146, 2009
16. G. S. Kumar, A. K. Shetty, K. Sambaiah, and P. V. Salimath, "Antidiabetic property of fenugreek seed mucilage and spent turmeric in streptozotocin-induced diabetic rats, " Nutrition Research, vol. 25, no. 11, pp. 1021-1028, 2005
17. T. Goswami, "A review on the functional properties, nutritional content, medicinal utilization and potential application of fenugreek, " Journal of Food Processing & Technology, vol. 3, no. 9, pp. 1-10, 2012
18. K. Bhatia, M. Kaur, F. Atif et al., "Aqueous extract of Trigonella foenum-graecum L. ameliorates additive urotoxicity of buthionine sulfoximine and cyclophosphamide in mice, " Food and Chemical Toxicology, vol. 44, no. 10, pp. 1744-1750, 2006
19. K.K.Khoja, G. Shafi, T. N. Hasan et al., "Fenugreek, a naturally occurring edible spice, kills MCF-7 human breast cancer cells via an apoptotic pathway, " Asian Pacific Journal of Cancer Prevention, vol. 12, no. 12, pp. 3299-3304, 2011
20. J. E.Thomas, M. Bandara, E. L. Lee, D. Driedger, and S.Acharya, "Biochemical monitoring in fenugreek to develop functional food and medicinal plant variants, " New Biotechnology, vol. 28, no. 2, pp. 110-117, 2011
21. A. Amin, A. Alkaabi, S. Al-Falasi, and S. A. Daoud, "Chemopreventive activities of Trigonella foenum graecum (Fenugreek) against breast cancer, " Cell Biology International, vol. 29, no. 8, pp. 687-694, 2005
22. S. F. S. Reihani and M. E. Azhar, "Antioxidant activity and total phenolic content in aqueous extracts of selected traditional mlay salads (Ulam), " International Food Research Journal, vol. 19, no. 4, pp. 1439-1444, 2012
23. J. Raju, J.M. R. Patlolla, M.V. Swamy, andC.V. Rao, "Diosgenin, a steroid saponin of Trigonella foenum graecum (Fenugreek), inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces apoptosis in HT-29 human colon cancer cells, " Cancer Epidemiology Biomarkers and Prevention, vol. 13, no. 8, pp. 1392-1398, 2004
24. S. Shishodia and B. B. Aggarwal, "Diosgenin inhibits osteoclastogenesis, invasion, and proliferation through the downregulation of Akt, I-B kinase activation and NF-B-regulated gene expression, " Oncogene, vol. 25, no. 10, pp. 1463-1473, 2006
25. R. D. Bhalke, S. J. Anarthe, K. D. Sasane, S. N. Satpute, S. N. Shinde, and V. S. Sangle, "Antinociceptive activity of Trigonella foenum-graecum leaves and seeds (Fabaceae), " Iranian Journal of Pharmacology & Therapeutics, vol. 8, no. 2, pp. 57-59, 2009
26. H. Hibasami, H. Moteki, K. Ishikawa et al., "Protodioscin isolated fromfenugreek (Trigonella foenum graecumL.) induces cell death and morphological change indicative of apoptosis in leukemic cell line H-60, but not in gastric cancer cell line KATO III, " International Journal ofMolecularMedicine, vol. 11, no. 1, pp. 23-26, 2003
27. Q. Yu, "Restoring p53-mediated apoptosis in cancer cells: new opportunities for cancer therapy, " Drug Resistance Updates, vol. 9, no. 1-2, pp. 19-25, 2006
28. K. H. Vousden and X. Lu, "Live or let die: the cell?s response to p53, " Nature Reviews Cancer, vol. 2, no. 8, pp. 594-604, 2002
29. I. O. L. Ng, E. C. S. Lai, A. S. Y. Chan, and M. K. P. So, "Overexpression of p53 in hepatocellular carcinomas: a clinicopathological and prognostic correlation, " Journal of Gastroenterology and Hepatology, vol. 10, no. 3, pp. 250-255, 1995
30. K. Breuhahn, T. Longerich, and P. Schirmacher, "Dysregulation of growth factor signaling in human hepatocellular carcinoma, " Oncogene, vol. 25, no. 27, pp. 3787-3800, 2006
31. F. Farinati, D. Marino, M. De Giorgio et al., "Diagnostic and prognostic role of -fetoprotein in hepatocellular carcinoma: both or neither?"The American Journal of Gastroenterology, vol. 101, no. 3, pp. 524-532, 2006
32. G. Guzman, V. Alagiozian-Angelova, J. E. Layden-Almer et al., "p53, Ki-67, and serum alpha feto-protein as predictors of hepatocellular carcinoma recurrence in liver transplant patients, " Modern Pathology, vol. 18, no. 11, pp. 1498-1503, 2005
33. I. Stoimenov and T. Helleday, "PCNA on the crossroad of cancer, " Biochemical Society Transactions, vol. 37, part 3, pp. 605-613, 2009
34. G. Loor, S.-J. Zhang, P. Zhang, N. Lan Toomey, and M. Y.W. T. Lee, "Identification of DNA replication and cell cycle proteins that interact with PCNA, " Nucleic Acids Research, vol. 25, no. 24, pp. 5041-5046, 1997
35. S.-C.Wang, Y. Nakajima, Y.-L. Yu et al., "Tyrosinephosphorylation controls PCNA function through protein stability, " Nature Cell Biology, vol. 8, no. 12, pp. 1359-1368, 2006
36. S. N. Naryzhny and H. Lee, "The post-translational modifications of proliferating cell nuclear antigen: acetylation, not phosphorylation, plays an important role in the regulation of its function, " The Journal of Biological Chemistry, vol. 279, no. 19, pp. 20194-20199, 2004
37. D. J. Hoelz, R. J. Arnold, L. E. Dobrolecki et al., "The discovery of labile methyl esters on proliferating cell nuclear antigen by MS/MS, " Proteomics, vol. 6, no. 17, pp. 4808-4816, 2006
38. S.-H. Peng, J.-F. Yang, P.-P. Xie, H. Deng, H. Li, and D.-Y. Feng, "Expression of cyclins in hepatocellular carcinoma and its correlation to tumor cell apoptosis, " Chinese Journal of Cancer, vol. 24, no. 6, pp. 695-698, 2005
39. A. Ashkenazi and V. M. Dixit, "Death receptors: signaling and modulation, " Science, vol. 281, no. 5381, pp. 1305-1308, 1998
40. M. D. Jacobson, M. Weil, and M. C. Raff, "Programmed cell death in animal development, " Cell, vol. 88, no. 3, pp. 347-354, 1997
41. X. Liu, H. Zou, C. Slaughter, and X. Wang, "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis, " Cell, vol. 89, no. 2, pp. 175-184, 1997
42. R. Datta, H. Kojima, K. Yoshida, and D. Kufe, "Caspase-3-mediated cleavage of protein kinase C - in induction of apoptosis, " The Journal of Biological Chemistry, vol. 272, no. 33, pp. 20317-20320, 1997
43. R. Bravo and H. Macdonald-Bravo, "Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: associationwithDNAreplication sites, "TheJournal ofCell Biology, vol. 105, no. 4, pp. 1549-1554, 1987
44. H. B. El-Serag, "Hepatocellular carcinoma: recent trends in the United States, " Gastroenterology, vol. 127, supplement 1, no. 5, pp. S27-S34, 2004
45. V. Thirunavukkarasu, C. V. Anuradha, and P. Viswanathan, "Protective effect of fenugreek (Trigonella foenum graecum) seeds in experimental ethanol toxicity, " Phytotherapy Research, vol. 17, no. 7, pp. 737-743, 2003
46. S. Kaviarasan and C. V. Anuradha, "Fenugreek (Trigonella foenum graecum) seed polyphenols protect liver from alcohol toxicity: a role on hepatic detoxification system and apoptosis, " Die Pharmazie, vol. 62, no. 4, pp. 299-304, 2007
47. S. K. Gupta, V. Kalaiselvan, S. Srivastava, R. Saxena, and S. S. Agrawal, "Trigonella foenum-graecum (fenugreek) protects against selenite-induced oxidative stress in experimental cataractogenesis, " Biological Trace Element Research, vol. 136, no. 3, pp. 258-268, 2010
48. P. Vijayan, P. Vijayaraj, P. H. C. Setty et al., "The cytotoxic activity of the total alkaloids isolated from different parts of Solanum pseudocapsicum, " Biological and Pharmaceutical Bulletin, vol. 27, no. 4, pp. 528-530, 2004
49. S. Vijayarathna and S. Sasidharan, "Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines, " Asian Pacific Journal of Tropical Biomedicine, vol. 2, no. 10, pp. 826-829, 2012
50. A. Das, N. L. Banik, and S. K. Ray, "Flavonoids activated caspases for apoptosis in human glioblastoma T98G and U87MG cells but not in human normal astrocytes, " Cancer, vol. 116, no. 1, pp. 164-176, 2010
51. G. McGill and D. E. Fisher, "Apoptosis in tumorigenesis and cancer therapy, " Frontiers in Bioscience, vol. 2, pp. 353-379, 1997
52. C. Corbiere, B. Liagre, F. Terro, and J.-L. Beneytout, "Induction of antiproliferative effect by diosgenin through activation of p53, release of apoptosis-inducing factor (AIF) andmodulation of caspase-3 activity in different human cancer cells, " Cell Research, vol. 14, no. 3, pp. 188-196, 2004
53. R. Aneja, A. M. Ghaleb, J. Zhou, V. W. Yang, and H. C. Joshi, "p53 and p21 determine the sensitivity of noscapine-induced apoptosis in colon cancer cells, " Cancer Research, vol. 67, no. 8, pp. 3862-3870, 2007
54. M. Cordenonsi, M. Montagner, M. Adorno et al., "Integration of TGF- and Ras/MAPK signaling through p53 phosphorylation, " Science, vol. 315, no. 5813, pp. 840-843, 2007
55. A. A. Alshatwi, G. Shafi, T. N. Hasan, N. A. Syed, and K. K. Khoja, "Fenugreek induced apoptosis in breast cancer MCF-7 cells mediated independently by fas receptor change, " Asian Pacific Journal of Cancer Prevention, vol. 14, no. 10, pp. 5783-5788, 2013
56. K. Boehme, Y. Dietz, P. Hewitt, and S. O. Mueller, "Activation of P53 in HepG2 cells as surrogate to detect mutagens and promutagens in vitro, " Toxicology Letters, vol. 198, no. 2, pp. 272-281, 2010
57. G. Maga and U. Hübscher, "Proliferating cell nuclear antigen (PCNA): a dancer with many partners, " Journal of Cell Science, vol. 116, no. 15, pp. 3051-3060, 2003
58. M. R. Hussein, "Alterations of p53, Bcl-2, and hMSH2 protein expression in the cirrhotic, macroregenerative, dysplastic nodules and hepatocellular carcinomas in Upper Egypt, " Liver International, vol. 24, no. 6, pp. 552-560, 2004
59. T.-H. Hu, C.-C.Wang, C.-C. Huang et al., "Down-regulation of tumor suppressor gene PTEN, overexpression of p53, plus high proliferating cell nuclear antigen index predict poor patient outcome of hepatocellular carcinoma after resection, " Oncology Reports, vol. 18, no. 6, pp. 1417-1426, 2007
60. H. J. Jung, H. L. Kim, Y. J. Kim, J.-I. Weon, and Y. R. Seo, "A novel chemopreventive mechanism of selenomethionine: enhancement of APE1 enzyme activity via a Gadd45a, PCNA and APE1 protein complex that regulates p53-mediated base excision repair, " Oncology Reports, vol. 30, no. 4, pp. 1581-1586, 2013
61. N. Azam, M. Vairapandi, W. Zhang, B. Hoffman, and D. A. Liebermann, "Interaction of CR6 (GADD45-) with proliferating cell nuclear antigen impedes negative growth control, " The Journal of Biological Chemistry, vol. 276, no. 4, pp. 2766-2774, 2001
62. H.-W. Chang, Y.-C.Lai, C.-Y. Cheng, J.-L.Ho, S.-T.Ding, andY.-C. Liu, "UV inducibility of rat proliferating cell nuclear antigen gene promoter, " Journal of Cellular Biochemistry, vol. 73, no. 3, pp. 423-432, 1999
63. C.V. Shivakumar, D. R. Brown, S.Deb, andS.P.Deb, "Wild-type human p53 transactivates the human proliferating cell nuclear antigen promoter, " Molecular and Cellular Biology, vol. 15, no. 12, pp. 6785-6793, 1995
64. T. Paunesku, S.Mittal, M. Protić et al., "Proliferating cell nuclear antigen (PCNA): ringmaster of the genome, " International Journal of Radiation Biology, vol. 77, no. 10, pp. 1007-1021, 2001
65. S. N. Naryzhny and H. Lee, "Observation of multiple isoforms and specific proteolysis patterns of proliferating cell nuclear antigen in the context of cell cycle compartments and sample preparations, " Proteomics, vol. 3, no. 6, pp. 930-936, 2003
66. L. Le Marchand, S. P. Murphy, J. H. Hankin, L. R.Wilkens, and L. N. Kolonel, "Intake of flavonoids and lung cancer, " Journal of the National Cancer Institute, vol. 92, no. 2, pp. 154-160, 2000
67. S.-I. Kanno, A. Tomizawa, T. Hiura et al., "Inhibitory effects of naringenin on tumor growth in human cancer cell lines and sarcoma S-180-implanted mice, " Biological and Pharmaceutical Bulletin, vol. 28, no. 3, pp. 527-530, 2005
68. A. K. Maurya and M. Vinayak, "Quercetin regresses Dalton?s lymphoma growth via suppression of PI3K/AKT signaling leading to upregulation of p53 and decrease in energy metabolism, " Nutrition and Cancer, vol. 67, no. 2, pp. 354-363, 2015