A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression

Genome Biology

Volumn 16, Issue 1, 2015, Pages

  Xi, Linghe ^a,b   Schmidt, Jens C ^a   Zaug, Arthur J ^a   Ascarrunz, Dante R ^a,b   Cech, Thomas R ^a,b  

^a UNIVERSITY OF COLORADO   (United States)

^b UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

Author keywords

Cajal body; Cancer mutations; CRISPR Cas9 genome editing; SNAP tag; Telomerase; TERT

Indexed keywords

TELOMERASE REVERSE TRANSCRIPTASE; TELOMERASE; TERT PROTEIN, HUMAN;

AMINO TERMINAL SEQUENCE; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CRISPR CAS SYSTEM; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENE INSERTION SEQUENCE; GENE LOCUS; GENOME ANALYSIS; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; PROMOTER REGION; PROTEIN MODIFICATION; TERT GENE; UPREGULATION; BIOSYNTHESIS; CHROMOSOME; DNA REPLICATION; GENETICS; HELA CELL LINE; HUMAN GENOME; MUTATION; RNA EDITING; TELOMERE;

CHROMOSOMES; CRISPR-CAS SYSTEMS; DNA REPLICATION; GENE EXPRESSION REGULATION; GENOME, HUMAN; HELA CELLS; HUMANS; MUTATION; PROMOTER REGIONS, GENETIC; RNA EDITING; TELOMERASE; TELOMERE;

EID: 84946567748     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/s13059-015-0791-1     Document Type: Article

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