Protein composition of catalytically active human telomerase from immortal cells

Science

Volumn 315, Issue 5820, 2007, Pages 1850-1853

  Cohen, Scott B ^a   Graham, Mark E ^a   Lovrecz, George O ^b   Bache, Nicolai ^c   Robinson, Phillip J ^a   Reddel, Roger R ^a  

^a CHILDREN'S MEDICAL RESEARCH INSTITUTE   (Australia)

^b CSIRO   (Australia)

^c UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

DYSKERIN; RIBONUCLEOPROTEIN; RNA; TELOMERASE; TELOMERASE REVERSE TRANSCRIPTASE; CELL CYCLE PROTEIN; DKC1 PROTEIN, HUMAN; MULTIENZYME COMPLEX; NUCLEAR PROTEIN; TELOMERASE RNA; UNCLASSIFIED DRUG;

CANCER; CATALYSIS; CHROMOSOME; ENZYME ACTIVITY; GENE EXPRESSION; MOLECULAR ANALYSIS; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL IMMORTALIZATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; MOLECULAR SIZE; PRIORITY JOURNAL; TELOMERE; CELL LINE; CHEMISTRY; DENSITY GRADIENT CENTRIFUGATION; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; TANDEM MASS SPECTROMETRY; TUMOR CELL LINE;

AMINO ACID SEQUENCE; CELL CYCLE PROTEINS; CELL LINE; CELL LINE, TUMOR; CENTRIFUGATION, DENSITY GRADIENT; HUMANS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MULTIENZYME COMPLEXES; NUCLEAR PROTEINS; RNA; TANDEM MASS SPECTROMETRY; TELOMERASE;

EID: 34147095342     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1138596     Document Type: Article

References (22)

1. R. K. Moyzis et al, Proc. Natl Acad. Sci. U.S.A. 85, 6622 (1988).
2. C. B. Harley, A. B. Futcher, C. W. Greider, Nature 345, 458 (1990).
3. A. M. Olovnikov, J. Theor. Biol. 41, 181 (1973).
4. C. W. Greider, E. H. Blackburn, Cell 43, 405 (1985).
5. J. Lingner et al., Science 276, 561 (1997).
6. T. M. Nakamura et al., Science 277, 955 (1997).
7. J. Feng et al., Science 269, 1236 (1995).
8. J. Lingner, T. R. Cech, Proc. Natl. Acad. Sci. U.S.A. 93, 10712 (1996).
9. C. Wenz et al., EMBO J. 20, 3526 (2001).
10. G. Schnapp, H. Rodi, W. J. Rettig, A. Schnapp, K. Damm, Nucleic Acids Res. 26, 3311 (1998).
11. Materials and methods are available as supporting material on Science Online.
12. G. Wallweber, S. Gryaznov, K. Pongracz, R. Pruzan, Biochemistry 42, 589 (2003).
13. T. M. Bryan, A. Englezou, M. A. Dunham, R. R. Reddel, Exp. Cell Res. 239, 370 (1998).
14. N. S. Heiss et al., Nat. Genet. 19, 32 (1998).
15. J. R. Mitchell, J. Cheng, K. Collins, Mol. Cell. Biol. 19, 567 (1999).
16. J. R. Mitchell, E. Wood, K. Collins, Nature 402, 551 (1999).
17. M. Armanios et al, Proc. Natl. Acad. Sci. USA 102, 15960 (2005).
18. T. Vulliamy et al., Nature 413, 432 (2001).
19. T. L. Beattie, W. Zhou, M. O. Robinson, L. Harrington, Mol. Cell. Biol. 2l, 6151 (2001).
20. K. Arai et al., J. Biol. Chem. 277, 8538 (2002).
21. Single-letter abbreviations for the amino add residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
22. We thank the laboratory of T. Bryan (Cell Biology Unit, Children's Medical Research Institute) for assistance with telomerase methods and L. Cheong, L. Lu, and T. Phan (Commonwealth Scientific and Industrial Research Organisation) for fermentation support. This research was supported by the Carcinogenesis Fellowship of the Cancer Council of New South Wales and by the National Health and Medical Research Council of Australia. The accession numbers for hTERT and human dyskerin are 014746 and CAA11970, respectively.