Current trends in mass spectrometry of peptides and proteins: Application to veterinary and sports-doping control

Mass Spectrometry Reviews

Volumn 34, Issue 6, 2015, Pages 571-594

  Van Den Broek, Irene ^a,b   Blokland, Marco ^a   Nessen, Merel A ^a   Sterk, Saskia ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

doping analysis; food regulation; growth promoters; hCG; IGF 1; peptide hormones

Indexed keywords

CONTROLLED DRUG DELIVERY; HORMONES; MASS SPECTROMETRY; PROTEOMICS; SPORTS;

DOPING ANALYSIS; GROWTH PROMOTERS; GROWTH-PROMOTING EFFECTS; IGF-1; PEPTIDE HORMONES; QUALITATIVE ANALYSIS; QUALITATIVE AND QUANTITATIVE ANALYSIS; QUALITATIVE AND QUANTITATIVE APPROACHES;

PEPTIDES;

GONADOTROPIN; GROWTH HORMONE; INSULIN; PEPTIDE; PLASMA PROTEIN; PROTEIN; SOMATOMEDIN C;

AMINO ACID SEQUENCE; ANIMAL; BLOOD; DOPING; HUMAN; MASS SPECTROMETRY; MOLECULAR GENETICS; PROCEDURES; PROTEINURIA; PROTEOMICS; SUBSTANCE ABUSE; URINE;

AMINO ACID SEQUENCE; ANIMALS; BLOOD PROTEINS; DOPING IN SPORTS; GONADOTROPINS; GROWTH HORMONE; HUMANS; INSULIN; INSULIN-LIKE GROWTH FACTOR I; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEINS; PROTEINURIA; PROTEOMICS; SUBSTANCE ABUSE DETECTION;

EID: 84942987988     PISSN: 02777037     EISSN: 10982787     Source Type: Journal    
DOI: 10.1002/mas.21419     Document Type: Review

References (157)

1. Abramson FP, Osborn BL, Teffera Y,. 1996. Isotopic differences in human growth hormone preparations. Anal Chem 68: 1971-1972.
2. Adachi J, Kumar C, Zhang Y, Olsen JV, Mann M,. 2006. The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins. Genome Biol 7: R80.
3. áDe Wasch K,. 1998. Multi-laboratory study of the analysis and kinetics of stanozolol and its metabolites in treated calves. Analyst 123: 2599-2604.
4. Aebersold R, Mann M,. 2003. Mass spectrometry-based proteomics. Nature 422: 198-207.
5. Altelaar AF, Frese CK, Preisinger C, Hennrich ML, Schram AW, Timmers HT, Heck AJ, Mohammed S,. 2012. Benchmarking stable isotope labeling based quantitative proteomics. J Proteomics 88: 14-26.
6. Altelaar AF, Munoz J, Heck AJ,. 2013. Next-generation proteomics: Towards an integrative view of proteome dynamics. Nat Rev Genet 14: 35-48.
7. Anderson NL, Anderson NG,. 2002. The human plasma proteome: History, character, and diagnostic prospects. Mol Cell Proteomics 1: 845-867.
8. Anderson NL, Anderson NG, Haines LR, Hardie DB, Olafson RW, Pearson TW,. 2004. Mass spectrometric quantitation of peptides and proteins using Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA). J Proteome Res 3: 235-244.
9. Aqai P, Cevik E, Gerssen A, Haasnoot W, Nielen MW,. 2013. High-throughput bioaffinity mass spectrometry for screening and identification of designer anabolic steroids in dietary supplements. Anal Chem 85: 3255-3262.
10. Arsene CG, Ohlendorf R, Burkitt W, Pritchard C, Henrion A, O'Connor G, Bunk DM, Guttler B,. 2008. Protein quantification by isotope dilution mass spectrometry of proteolytic fragments: Cleavage rate and accuracy. Anal Chem 80: 4154-4160.
11. Arsene CG, Henrion A, Diekmann N, Manolopoulou J, Bidlingmaier M,. 2010. Quantification of growth hormone in serum by isotope dilution mass spectrometry. Anal Biochem 401: 228-235.
12. Arsene CG, Schulze D, Kratzsch J, Henrion A,. 2012. High sensitivity mass spectrometric quantification of serum growth hormone by amphiphilic peptide conjugation. J Mass Spectrom 47: 1554-1560.
13. Ayotte C, Goudreault D,. 1999. The detection of new synthetic drugs in athlete's urine samples. Toxin Rev 18: 113-123.
14. Bailly-Chouriberry L, Chu-Van E, Pinel G, Garcia P, Popot MA, Andre-Fontaine G, Bonnaire Y, Le Bizec B,. 2008a. Detection of secondary biomarker of met-eGH as a strategy to screen for somatotropin misuse in horseracing. Analyst 133: 270-276.
15. Bailly-Chouriberry L, Pinel G, Garcia P, Popot MA, Le Bizec B, Bonnaire Y,. 2008b. Identification of recombinant equine growth hormone in horse plasma by LC-MS/MS: A confirmatory analysis in doping control. Anal Chem 80: 8340-8347.
16. Barroso O, Schamasch P, Rabin O,. 2009. Detection of GH abuse in sport: Past, present and future. Growth Horm IGF Res 19: 369-374.
17. Barroso O, Handelsman DJ, Strasburger C, Thevis M,. 2012. Analytical challenges in the detection of peptide hormones for anti-doping purposes. Bioanalysis 4: 1577-1590.
18. Barton C, Kay RG, Gentzer W, Vitzthum F, Pleasance S,. 2010. Development of high-throughput chemical extraction techniques and quantitative HPLC-MS/MS (SRM) assays for clinically relevant plasma proteins. J Proteome Res 9: 333-340.
19. Bauman DE,. 1999. Bovine somatotropin and lactation: From basic science to commercial application. Domest Anim Endocrinol 17: 101-116.
20. Baumann GP,. 2012. Growth hormone doping in sports: A critical review of use and detection strategies. Endocr Rev 33: 155-186.
21. Bereman MS, Canterbury JD, Egertson JD, Horner J, Remes PM, Schwartz J, Zabrouskov V, MacCoss MJ,. 2012. Evaluation of front-end higher energy collision-induced dissociation on a benchtop dual-pressure linear ion trap mass spectrometer for shotgun proteomics. Anal Chem 84: 1533-1539.
22. Beynon RJ, Doherty MK, Pratt JM, Gaskell SJ,. 2005. Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides. Nat Methods 2: 587-589.
23. Birkeland KI, Hemmersbach P,. 1999. The future of doping control in athletes. Issues related to blood sampling. Sports Med 28: 25-33.
24. Blokland MH, Sterk SS, van Ginkel LA, Stephany RW, Heck A,. 2003. Analysis for endogenous and recombinant porcine somatotropine in serum. Anal Chim Acta 483: 201-206.
25. Blokland MH, Zomer G, Sterk SS, Herbold HA, Wubs KL, Ginkel LA, Stephany RW,. 2004. Identification of a novel pharmacological active agent in an illegal growth promoting preparation. EuroResidue V. Global aspects of residues. Proceedings of the conference on residues of veterinary drugs in food, Noordwijkerhout, The Netherlands, pp. 127-134.
26. Blokland MH, Wubs KL, Nessen MA, Sterk SS, Nielen MW,. 2013. Analysis of endogenous and recombinant bovine somatotropin in serum. Farm animal proteomics 2013. Wageningen: Wageningen Academic Publishers. pp. 209-212.
27. Bobin S, Popot MA, Bonnaire Y, Tabet JC,. 2001. Approach to the determination of insulin-like-growth-factor-I (IGF-I) concentration in plasma by high-performance liquid chromatography-ion trap mass spectrometry: Use of a deconvolution algorithm for the quantification of multiprotonated molecules in electrospray ionization. Analyst 126: 1996-2001.
28. Boschetti E, Righetti PG,. 2009. The art of observing rare protein species in proteomes with peptide ligand libraries. Proteomics 9: 1492-1510.
29. Boulo S, Hanisch K, Bidlingmaier M, Arsene CG, Panteghini M, Auclair G, Sturgeon C, Schimmel H, Zegers I,. 2013. Gaps in the traceability chain of human growth hormone measurements. Clin Chem 59: 1074-1082.
30. Bovee T, Pikkemaat M,. 2009. Bioactivity-based screening of antibiotics and hormones. J Chromatogr A 1216: 8035-8050.
31. Bowers CY,. 1998. Growth hormone-releasing peptide (GHRP). Cell Mol Life Sci 54: 1316-1329.
32. Bredehoft M, Schanzer W, Thevis M,. 2008. Quantification of human insulin-like growth factor-1 and qualitative detection of its analogues in plasma using liquid chromatography/electrospray ionisation tandem mass spectrometry. Rapid Commun Mass Spectrom 22: 477-485.
33. Bronsema KJ, Bischoff R, van de Merbel NC,. 2012. Internal standards in the quantitative determination of protein biopharmaceuticals using liquid chromatography coupled to mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 893-894: 1-14.
34. Brun V, Dupuis A, Adrait A, Marcellin M, Thomas D, Court M, Vandenesch F, Garin J,. 2007. Isotope-labeled protein standards: Toward absolute quantitative proteomics. Mol Cell Proteomics 6: 2139-2149.
35. Bystrom CE, Sheng S, Clarke NJ,. 2011. Narrow mass extraction of time-of-flight data for quantitative analysis of proteins: Determination of insulin-like growth factor-1. Anal Chem 83: 9005-9010.
36. Bystrom C, Sheng S, Zhang K, Caulfield M, Clarke NJ, Reitz R,. 2012. Clinical utility of insulin-like growth factor 1 and 2; determination by high resolution mass spectrometry. PLoS ONE 7: e43457.
37. Campbell R, Steele N, Caperna T, McMurtry J, Solomon M, Mitchell A,. 1989. Interrelationships between sex and exogenous growth hormone administration on performance, body composition and protein and fat accretion of growing pigs. J Anim Sci 67: 177-186.
38. Chambers EE, Legido-Quigley C, Smith N, Fountain KJ,. 2013. Development of a fast method for direct analysis of intact synthetic insulins in human plasma: The large peptide challenge. Bioanalysis 5: 65-81.
39. Chen Z, Caulfield MP, McPhaul MJ, Reitz RE, Taylor SW, Clarke NJ,. 2013. Quantitative insulin analysis using liquid chromatography-tandem mass spectrometry in a high-throughput clinical laboratory. Clin Chem 59: 1349-1356.
40. Clemmons DR,. 2011. Consensus statement on the standardization and evaluation of growth hormone and insulin-like growth factor assays. Clin Chem 57: 555-559.
41. Court M, Selevsek N, Matondo M, Allory Y, Garin J, Masselon CD, Domon B,. 2011. Toward a standardized urine proteome analysis methodology. Proteomics 11: 1160-1171.
42. Cox HD, Eichner D,. 2013a. Detection of human insulin-like growth factor-1 in deer antler velvet supplements. Rapid Commun Mass Spectrom 27: 2170-2178.
43. Cox HD, Rampton J, Eichner D,. 2013b. Quantification of insulin-like growth factor-1 in dried blood spots for detection of growth hormone abuse in sport. Anal Bioanal Chem 405: 1949-1958.
44. Darby SM, Miller ML, Allen RO, LeBeau M,. 2001. A mass spectrometric method for quantitation of intact insulin in blood samples. J Anal Toxicol 25: 8-14.
45. de Kock SS, Rodgers JP, Swanepoel BC,. 2001. Growth hormone abuse in the horse: Preliminary assessment of a mass spectrometric procedure for IGF-1 identification and quantitation. Rapid Commun Mass Spectrom 15: 1191-1197.
46. Della Donna L, Ronci M, Sacchetta P, Di Ilio C, Biolatti B, Federici G, Nebbia C, Urbani A,. 2009. A food safety control low mass-range proteomics platform for the detection of illicit treatments in veal calves by MALDI-TOF-MS serum profiling. Biotechnol J 4: 1596-1609.
47. Di Girolamo F, Righetti PG, Soste M, Feng Y, Picotti P,. 2013. Reproducibility of combinatorial peptide ligand libraries for proteome capture evaluated by selected reaction monitoring. J Proteomics 89: 215-226.
48. Ding J, List EO, Okada S, Kopchick JJ,. 2009. Perspective: Proteomic approach to detect biomarkers of human growth hormone. Growth Horm IGF Res 19: 399-407.
49. Domon B, Aebersold R,. 2010. Options and considerations when selecting a quantitative proteomics strategy. Nat Biotechnol 28: 710-721.
50. Esposito S, Deventer K, Van Eenoo P,. 2012. Characterization and identification of a C-terminal amidated mechano growth factor (MGF) analogue in black market products. Rapid Commun Mass Spectrom 26: 686-692.
51. Etherton T, Wiggins J, Chung C, Evock C, Rebhun J, Walton P,. 1986. Stimulation of pig growth performance by porcine growth hormone and growth hormone-releasing factor. J Anim Sci 63: 1389-1399.
52. Etherton TD, Wiggins JP, Evock CM, Chung CS, Rebhun JF, Walton PE, Steele NC,. 1987. Stimulation of pig growth performance by porcine growth hormone: Determination of the dose-response relationship. J Anim Sci 64: 433.
53. Fedoruk MN, Rupert JL,. 2008. Myostatin inhibition: A potential performance enhancement strategy ? Scand J Med Sci Sports 18: 123-131.
54. Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM,. 1989. Electrospray ionization for mass spectrometry of large biomolecules. Science 246: 64-71.
55. Ferchaud V, Le Bizec B, Monteau F, André F,. 1998. Determination of the exogenous character of testosterone in bovine urine by gas chromatography-combustion-isotope ratio mass spectrometry. Analyst 123: 2617-2620.
56. Friedmann T, Flenker U, Georgakopoulos C, Alsayrafi M, Sottas P-E, Williams SA, Gill RD,. 2012. Evolving concepts and techniques for anti-doping. Bioanalysis 4: 1667-1680.
57. Gallien S, Duriez E, Crone C, Kellmann M, Moehring T, Domon B,. 2012. Targeted proteomic quantification on quadrupole-orbitrap mass spectrometer. Mol Cell Proteomics 11: 1709-1723.
58. Gallo P, Vinci F, Fusco G, Serpe L,. 2009. An analytical strategy for identification of a somatotropin-like bioactive peptide by ion trap liquid chromatography/electrospray ionization tandem mass spectrometry after immuno-affinity purification from buffalo serum. Rapid Commun Mass Spectrom 23: 395-402.
59. Gam LH, Tham SY, Latiff A,. 2003. Immunoaffinity extraction and tandem mass spectrometric analysis of human chorionic gonadotropin in doping analysis. J Chromatogr B Analyt Technol Biomed Life Sci 792: 187-196.
60. Gerber SA, Rush J, Stemman O, Kirschner MW, Gygi SP,. 2003. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc Natl Acad Sci USA 100: 6940-6945.
61. Ghigo E, Arvat E, Muccioli G, Camanni F,. 1997. Growth hormone-releasing peptides. Eur J Endocrinol 136: 445-460.
62. Gil J, Cabrales A, Reyes O, Morera V, Betancourt L, Sanchez A, Garcia G, Moya G, Padron G, Besada V, Gonzalez LJ,. 2012. Development and validation of a bioanalytical LC-MS method for the quantification of GHRP-6 in human plasma. J Pharm Biomed Anal 60: 19-25.
63. Godin JP, Fay LB, Hopfgartner G,. 2007. Liquid chromatography combined with mass spectrometry for 13C isotopic analysis in life science research. Mass Spectrom Rev 26: 751-774.
64. Guha N, Sonksen PH, Holt RI,. 2009. IGF-I abuse in sport: Current knowledge and future prospects for detection. Growth Horm IGF Res 19: 408-411.
65. Han J, Sun J, Sha C, Zhang J, Gai Y, Li Y, Liu W,. 2012. Quantitation of slow release triptorelin in beagle dog plasma by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal 66: 334-338.
66. Handelsman DJ,. 2006. Clinical review: The rationale for banning human chorionic gonadotropin and estrogen blockers in sport. J Clin Endocrinol Metab 91: 1646-1653.
67. Hanke S, Besir H, Oesterhelt D, Mann M,. 2008. Absolute SILAC for accurate quantitation of proteins in complex mixtures down to the attomole level. J Proteome Res 7: 1118-1130.
68. Henninge J, Pepaj M, Hullstein I, Hemmersbach P,. 2010. Identification of CJC-1295, a growth-hormone-releasing peptide, in an unknown pharmaceutical preparation. Drug Test Anal 2: 647-650.
69. Hess C, Thomas A, Thevis M, Stratmann B, Quester W, Tschoepe D, Madea B, Musshoff F,. 2012. Simultaneous determination and validated quantification of human insulin and its synthetic analogues in human blood serum by immunoaffinity purification and liquid chromatography-mass spectrometry. Anal Bioanal Chem 404: 1813-1822.
70. Ho EN, Wan TS, Wong AS, Lam KK, Stewart BD,. 2008. Doping control analysis of insulin and its analogues in equine plasma by liquid chromatography-tandem mass spectrometry. J Chromatogr A 1201: 183-190.
71. Ho EN, Wan TS, Wong AS, Lam KK, Stewart BD,. 2011. Doping control analysis of insulin and its analogues in equine urine by liquid chromatography-tandem mass spectrometry. J Chromatogr A 1218: 1139-1146.
72. Ho EN, Kwok WH, Lau MY, Wong AS, Wan TS, Lam KK, Schiff PJ, Stewart BD,. 2012. Doping control analysis of TB-500, a synthetic version of an active region of thymosin beta(4), in equine urine and plasma by liquid chromatography-mass spectrometry. J Chromatogr A 1265: 57-69.
73. Hoofnagle AN, Wener MH,. 2009. The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry. J Immunol Methods 347: 3-11.
74. Janssens G, Mangelinckx S, Courtheyn D, Prévost S, De Poorter G, De Kimpe N, Le Bizec B,. 2013. Application of gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) to detect the abuse of 17β-estradiol in cattle. J Agric Food Chem 61: 7242-7249.
75. Jones JI, Clemmons DR,. 1995. Insulin-like growth-factors and their binding-proteins-Biological actions. Endocr Rev 16: 3-34.
76. Karas M, Hillenkamp F,. 1988. Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal Chem 60: 2299-2301.
77. Kay RG, Creaser CS,. 2010. Application of mass spectrometry-based proteomics techniques for the detection of protein doping in sports. Expert Rev Proteomics 7: 185-188.
78. Kay RG, Barton C, Velloso CP, Brown PR, Bartlett C, Blazevich AJ, Godfrey RJ, Goldspink G, Rees R, Ball GR, Cowan DA, Harridge SD, Roberts J, Teale P, Creaser CS,. 2009. High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration. Rapid Commun Mass Spectrom 23: 3173-3182.
79. Kay R, Halsall DJ, Annamalai AK, Kandasamy N, Taylor K, Fenwick S, Webb A, Wark G, Pleasance S, Gurnell M,. 2013. A novel mass spectrometry-based method for determining insulin-like growth factor 1: Assessment in a cohort of subjects with newly diagnosed acromegaly. Clin Endocrinol (Oxf) 78: 424-430.
80. Kentsis A, Shulman A, Ahmed S, Brennan E, Monuteaux MC, Lee YH, Lipsett S, Paulo JA, Dedeoglu F, Fuhlbrigge R, Bachur R, Bradwin G, Arditi M, Sundel RP, Newburger JW, Steen H, Kim S,. 2013. Urine proteomics for discovery of improved diagnostic markers of Kawasaki disease. EMBO Mol Med 5: 210-220.
81. Keshishian H, Addona T, Burgess M, Kuhn E, Carr SA,. 2007. Quantitative, multiplexed assays for low abundance proteins in plasma by targeted mass spectrometry and stable isotope dilution. Mol Cell Proteomics 6: 2212-2229.
82. Kieken F, Pinel G, Antignac J-P, Paris A-C, Garcia P, Popot M-A, Grall M, Mercadier V, Toutain PL, Bonnaire Y,. 2011. Generation and processing of urinary and plasmatic metabolomic fingerprints to reveal an illegal administration of recombinant equine growth hormone from LC-HRMS measurements. Metabolomics 7: 84-93.
83. Kim MK, Lee TH, Suh JH, Eom HY, Min JW, Yeom H, Kim U, Jung HJ, Cha KH, Choi YS, Youm JR, Han SB,. 2010. Development and validation of a liquid chromatography-tandem mass spectrometry method for the determination of goserelin in rabbit plasma. J Chromatogr B Analyt Technol Biomed Life Sci 878: 2235-2242.
84. Kippen AD, Cerini F, Vadas L, Stocklin R, Vu L, Offord RE, Rose K,. 1997. Development of an isotope dilution assay for precise determination of insulin, C-peptide, and proinsulin levels in non-diabetic and type II diabetic individuals with comparison to immunoassay. J Biol Chem 272: 12513-12522.
85. Kirsch S, Widart J, Louette J, Focant JF, De Pauw E,. 2007. Development of an absolute quantification method targeting growth hormone biomarkers using liquid chromatography coupled to isotope dilution mass spectrometry. J Chromatogr A 1153: 300-306.
86. Kohler M, Thomas A, Geyer H, Petrou M, Schanzer W, Thevis M,. 2010a. Confiscated black market products and nutritional supplements with non-approved ingredients analyzed in the Cologne Doping Control Laboratory 2009. Drug Test Anal 2: 533-537.
87. Kohler M, Thomas A, Walpurgis K, Terlouw K, Schanzer W, Thevis M,. 2010b. Detection of His-tagged Long-R(3)-IGF-I in a black market product. Growth Horm IGF Res 20: 386-390.
88. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K,. 1999. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 402: 656-660.
89. Krastins B, Prakash A, Sarracino DA, Nedelkov D, Niederkofler EE, Kiernan U, Nelson R, Vogelsang MS, Vadali G, Garces A, Sutton JN, Peterman S, Byram G, Darbouret B, Coulombe B, Gobom J, Portelius E, Pannee J, Blennow K, Kulasingam V, Couchman L, Moniz C, Lopez MF,. 2013. Rapid development of sensitive, high-throughput, quantitative and highly selective mass spectrometric targeted immunoassays for clinically important proteins in human plasma and serum. Clin Biochem 46: 12.
90. Kuuranne T, Thomas A, Leinonen A, Delahaut P, Bosseloir A, Schanzer W, Thevis M,. 2008. Insulins in equine urine: Qualitative analysis by immunoaffinity purification and liquid chromatography/tandem mass spectrometry for doping control purposes in horse-racing. Rapid Commun Mass Spectrom 22: 355-362.
91. Kwok WH, Ho EN, Lau MY, Leung GN, Wong AS, Wan TS,. 2013. Doping control analysis of seven bioactive peptides in horse plasma by liquid chromatography-mass spectrometry. Anal Bioanal Chem 405: 2595-2606.
92. Le Breton MH, Rochereau-Roulet S, Pinel G, Bailly-Chouriberry L, Rychen G, Jurjanz S, Goldmann T, Le Bizec B,. 2008. Direct determination of recombinant bovine somatotropin in plasma from a treated goat by liquid chromatography/high-resolution mass spectrometry. Rapid Commun Mass Spectrom 22: 3130-3136.
93. Le Breton MH, Rochereau-Roulet S, Chereau S, Pinel G, Delatour T, Le Bizec B,. 2010. Identification of cows treated with recombinant bovine somatotropin. J Agric Food Chem 58: 729-733.
94. Lempiainen A, Hotakainen K, Alfthan H, Stenman UH,. 2012. Loss of human chorionic gonadotropin in urine during storage at -20C. Clin Chim Acta 413: 232-236.
95. Liu Z, Ren C, Jones W, Chen P, Seminara SB, Chan YM, Smith NF, Covey JM, Wang J, Chan KK,. 2013. LC-MS/MS quantification of a neuropeptide fragment kisspeptin-10 (NSC 741805) and characterization of its decomposition product and pharmacokinetics in rats. J Chromatogr B Analyt Technol Biomed Life Sci 926: 1-8.
96. Longobardi S, Keay N, Ehrnborg C, Cittadini A, Rosen T, Dall R, Boroujerdi MA, Bassett EE, Healy ML, Pentecost C, Wallace JD, Powrie J, Jorgensen JO, Sacca L,. 2000. Growth hormone (GH) effects on bone and collagen turnover in healthy adults and its potential as a marker of GH abuse in sports: A double blind, placebo-controlled study. The GH-2000 Study Group. J Clin Endocrinol Metab 85: 1505-1512.
97. Lucy MC, Stevenson JS,. 1986. Gonadotropin-releasing hormone at estrus: Luteinizing hormone, estradiol, and progesterone during the periestrual and postinsemination periods in dairy cattle. Biol Reprod 35: 300-311.
98. Ludwig SK, Smits NG, van der Veer G, Bremer MG, Nielen MW,. 2012. Multiple protein biomarker assessment for recombinant bovine somatotropin (rbST) abuse in cattle. PLoS ONE 7: e52917.
99. Lund H, Torsetnes SB, Paus E, Nustad K, Reubsaet L, Halvorsen TG,. 2009. Exploring the complementary selectivity of immunocapture and MS detection for the differentiation between hCG isoforms in clinically relevant samples. J Proteome Res 8: 5241-5252.
100. Lund H, Lovsletten K, Paus E, Halvorsen TG, Reubsaet L,. 2012. Immuno-MS based targeted proteomics: Highly specific, sensitive, and reproducible human chorionic gonadotropin determination for clinical diagnostics and doping analysis. Anal Chem 84: 7926-7932.
101. Lund H, Snilsberg AH, Halvorsen TG, Hemmersbach P, Reubsaet L,. 2013a. Comparison of newly developed immuno-MS method with existing DELFIA((R)) immunoassay for human chorionic gonadotropin determination in doping analysis. Bioanalysis 5: 623-630.
102. Lund H, Snilsberg AH, Paus E, Halvorsen TG, Hemmersbach P, Reubsaet L,. 2013b. Sports drug testing using immuno-MS: Clinical study comprising administration of human chorionic gonadotropin to males. Anal Bioanal Chem 405: 1569-1576.
103. Marcovina S, Bowsher RR, Miller WG, Staten M, Myers G, Caudill SP, Campbell SE, Steffes MW, Insulin Standardization Workgroup. 2007. Standardization of insulin immunoassays: Report of the American Diabetes Association Workgroup. Clin Chem 53: 711-716.
104. Miguel GZ, Faria MH, Roça RO, Santos CT, Suman SP, Faitarone AB, Delbem NL, Girao LV, Homem JM, Barbosa EK,. 2013. Immunocastration improves carcass traits and beef color attributes in Nellore and Nellore × Aberdeen Angus crossbred animals finished in feedlot. Meat Sci 96: 884-891.
105. Moller I, Thomas A, Geyer H, Schanzer W, Thevis M,. 2012. Development and validation of a mass spectrometric detection method of peginesatide in dried blood spots for sports drug testing. Anal Bioanal Chem 403: 2715-2724.
106. Nagaraj N, Mann M,. 2011. Quantitative analysis of the intra- and inter-individual variability of the normal urinary proteome. J Proteome Res 10: 637-645.
107. Nelson RW, Nedelkov D, Tubbs KA, Kiernan UA,. 2004. Quantitative mass spectrometric immunoassay of insulin like growth factor 1. J Proteome Res 3: 851-855.
108. Neubert H, Muirhead D, Kabir M, Grace C, Cleton A, Arends R,. 2013. Sequential protein and peptide immunoaffinity capture for mass spectrometry-based quantification of total human beta-nerve growth factor. Anal Chem 85: 1719-1726.
109. Okano M, Sato M, Ikekita A, Kageyama S,. 2010. Determination of growth hormone secretagogue pralmorelin (GHRP-2) and its metabolite in human urine by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom 24: 2046-2056.
110. Ong SE, Mann M,. 2005. Mass spectrometry-based proteomics turns quantitative. Nat Chem Biol 1: 252-262.
111. Peel CJ, Bauman DE, Gorewit RC, Sniffen CJ,. 1981. Effect of exogenous growth hormone on lactational performance in high yielding dairy cows. J Nutr 111: 1662.
112. Pinel G, Buon R, Aviat F, Larre C, Andre-Fontaine G, Andre F, Le Bizec B,. 2005. Recombinant bovine somatotropin misuse in cattle-Evaluation of Western blotting and 2D electrophoresis methods on biological samples for the demonstration of its administration. Anal Chim Acta 529: 41-46.
113. Popot MA, Bobin S, Bonnaire Y, Delahaut PH, Closset J,. 2001. IGF-I plasma concentrations in non-treated horses and horses administered with methionyl equine somatotropin. Res Vet Sci 71: 167-173.
114. Pottgiesser T, Schumacher YO,. 2012. Biomarker monitoring in sports doping control. Bioanalysis 4: 1245-1253.
115. Powrie JK, Bassett EE, Rosen T, Jorgensen JO, Napoli R, Sacca L, Christiansen JS, Bengtsson BA, Sonksen PH,. 2007. Detection of growth hormone abuse in sport. Growth Horm IGF Res 17: 220-226.
116. Reichel C,. 2011. OMICS-strategies and methods in the fight against doping. Forensic Sci Int 213: 20-34.
117. Rodriguez-Cabaleiro D, Van Uytfanghe K, Stove V, Fiers T, Thienpont LM,. 2007. Pilot study for the standardization of insulin immunoassays with isotope dilution liquid chromatography/tandem mass spectrometry. Clin Chem 53: 1462-1469.
118. Schams D, Graf F, Meyer J, Graule B, Mauthner M, Wollny C,. 1991. Changes in hormones, metabolites, and milk after treatment with sometribove (recombinant methionyl bST) in Deutsches Fleckvieh and German black and white cows. J Anim Sci 69: 1583-1592.
119. Singh S, Springer M, Steen J, Kirschner MW, Steen H,. 2009. FLEXIQuant: A novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. J Proteome Res 8: 2201-2210.
120. Smits NG, Bremer MG, Ludwig SK, Nielen MW,. 2012. Development of a flow cytometric immunoassay for recombinant bovine somatotropin-induced antibodies in serum of dairy cows. Drug Test Anal 4: 362-367.
121. Smits NG, Ludwig SK, Van der Veer G, Bremer MG, Nielen MW,. 2013. Multiplex flow cytometric immunoassay for serum biomarker profiling of recombinant bovine somatotropin. Analyst 138: 111-117.
122. Sonksen PH,. 2001. Insulin, growth hormone and sport. J Endocrinol 170: 13-25.
123. Stenman UH, Unkila-Kallio L, Korhonen J, Alfthan H,. 1997. Immunoprocedures for detecting human chorionic gonadotropin: clinical aspects and doping control. Clin Chem 43: 1293-1298.
124. Stenman UH, Tiitinen A, Alfthan H, Valmu L,. 2006. The classification, functions and clinical use of different isoforms of HCG. Hum Reprod Update 12: 769-784.
125. Stenman UH, Hotakainen K, Alfthan H,. 2008. Gonadotropins in doping: Pharmacological basis and detection of illicit use. Br J Pharmacol 154: 569-583.
126. Stephany RW,. 2010. Hormonal growth promoting agents in food producing animals. Handb Exp Pharmacol 195: 355-367.
127. Stocklin R, Vu L, Vadas L, Cerini F, Kippen AD, Offord RE, Rose K,. 1997. A stable isotope dilution assay for the in vivo determination of insulin levels in humans by mass spectrometry. Diabetes 46: 44-50.
128. Thevis M, Thomas A, Delahaut P, Bosseloir A, Schanzer W,. 2005. Qualitative determination of synthetic analogues of insulin in human plasma by immunoaffinity purification and liquid chromatography-tandem mass spectrometry for doping control purposes. Anal Chem 77: 3579-3585.
129. Thevis M, Thomas A, Delahaut P, Bosseloir A, Schanzer W,. 2006. Doping control analysis of intact rapid-acting insulin analogues in human urine by liquid chromatography-tandem mass spectrometry. Anal Chem 78: 1897-1903.
130. Thevis M, Thomas A, Schanzer W,. 2008. Mass spectrometric determination of insulins and their degradation products in sports drug testing. Mass Spectrom Rev 27: 35-50.
131. Thevis M, Thomas A, Schanzer W,. 2010. Insulin. Handb Exp Pharmacol 195: 209-226.
132. Thevis M, Thomas A, Schanzer W,. 2011. Doping control analysis of selected peptide hormones using LC-MS(/MS). Forensic Sci Int 213: 35-41.
133. Thomas A, Thevis M, Delahaut P, Bosseloir A, Schanzer W,. 2007. Mass spectrometric identification of degradation products of insulin and its long-acting analogues in human urine for doping control purposes. Anal Chem 79: 2518-2524.
134. Thomas A, Geyer H, Kamber M, Schanzer W, Thevis M,. 2008. Mass spectrometric determination of gonadotrophin-releasing hormone (GnRH) in human urine for doping control purposes by means of LC-ESI-MS/MS. J Mass Spectrom 43: 908-915.
135. Thomas A, Schanzer W, Delahaut P, Thevis M,. 2009. Sensitive and fast identification of urinary human, synthetic and animal insulin by means of nano-UPLC coupled with high-resolution/high-accuracy mass spectrometry. Drug Test Anal 1: 219-227.
136. Thomas A, Guddat S, Kohler M, Krug O, Schanzer W, Petrou M, Thevis M,. 2010a. Comprehensive plasma-screening for known and unknown substances in doping controls. Rapid Commun Mass Spectrom 24: 1124-1132.
137. Thomas A, Kohler M, Mester J, Geyer H, Schanzer W, Petrou M, Thevis M,. 2010b. Identification of the growth-hormone-releasing peptide-2 (GHRP-2) in a nutritional supplement. Drug Test Anal 2: 144-148.
138. Thomas A, Hoppner S, Geyer H, Schanzer W, Petrou M, Kwiatkowska D, Pokrywka A, Thevis M,. 2011a. Determination of growth hormone releasing peptides (GHRP) and their major metabolites in human urine for doping controls by means of liquid chromatography mass spectrometry. Anal Bioanal Chem 401: 507-516.
139. Thomas A, Kohler M, Schanzer W, Delahaut P, Thevis M,. 2011b. Determination of IGF-1 and IGF-2, their degradation products and synthetic analogues in urine by LC-MS/MS. Analyst 136: 1003-1012.
140. Thomas A, Schanzer W, Delahaut P, Thevis M,. 2011c. Immunoaffinity purification of peptide hormones prior to liquid chromatography-mass spectrometry in doping controls. Methods 56: 230-235.
141. Thomas A, Delahaut P, Krug O, Schanzer W, Thevis M,. 2012a. Metabolism of growth hormone releasing peptides. Anal Chem 84: 10252-10259.
142. Thomas A, Walpurgis K, Krug O, Schanzer W, Thevis M,. 2012b. Determination of prohibited, small peptides in urine for sports drug testing by means of nano-liquid chromatography/benchtop quadrupole orbitrap tandem-mass spectrometry. J Chromatogr A 1259: 251-257.
143. van den Broek I, Sparidans RW, Schellens JH, Beijnen JH,. 2008. Quantitative bioanalysis of peptides by liquid chromatography coupled to (tandem) mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 872: 1-22.
144. van den Broek I, Niessen WM, van Dongen WD,. 2013a. Bioanalytical LC-MS/MS of protein-based biopharmaceuticals. J Chromatogr B Analyt Technol Biomed Life Sci 929: 161-179.
145. van den Broek I, Smit NP, Romijn FP, van der Laarse A, Deelder AM, van der Burgt YE, Cobbaert CM,. 2013b. Evaluation of inter-specimen trypsin digestion efficiency prior to MRM-based absolute protein quantification with native protein calibrators. J Proteome Res, DOI: 10.1021/pr400763d [Epub ahead of print].
146. Van Oudenhove L, Devreese B,. 2013. A review on recent developments in mass spectrometry instrumentation and quantitative tools advancing bacterial proteomics. Appl Microbiol Biotechnol 97: 4749-4762.
147. Van Uytfanghe K, Rodriguez-Cabaleiro D, Stockl D, Thienpont LM,. 2007. New liquid chromatography/electrospray ionisation tandem mass spectrometry measurement procedure for quantitative analysis of human insulin in serum. Rapid Commun Mass Spectrom 21: 819-821.
148. Vesper HW, Miller WG, Myers GL,. 2007. Reference materials and commutability. Clin Biochem Rev 28: 139-147.
149. Wallace JD, Cuneo RC, Baxter R, Orskov H, Keay N, Pentecost C, Dall R, Rosen T, Jorgensen JO, Cittadini A, Longobardi S, Sacca L, Christiansen JS, Bengtsson BA, Sonksen PH,. 1999. Responses of the growth hormone (GH) and insulin-like growth factor axis to exercise, GH administration, and GH withdrawal in trained adult males: A potential test for GH abuse in sport. J Clin Endocrinol Metab 84: 3591-3601.
150. Walpurgis K, Thomas A, Laussmann T, Horta L, Metzger S, Schanzer W, Thevis M,. 2011. Identification of fibroblast growth factor 1 (FGF-1) in a black market product. Drug Test Anal 3: 791-797.
151. Wu Z, Bidlingmaier M, Dall R, Strasburger CJ,. 1999. Detection of doping with human growth hormone. Lancet 353: 895.
152. Yang Z, Hayes M, Fang X, Daley MP, Ettenberg S, Tse FL,. 2007. LC-MS/MS approach for quantification of therapeutic proteins in plasma using a protein internal standard and 2D-solid-phase extraction cleanup. Anal Chem 79: 9294-9301.
153. Yates JR, Ruse CI, Nakorchevsky A,. 2009. Proteomics by mass spectrometry: Approaches, advances, and applications. Annu Rev Biomed Eng 11: 49-79.
154. Zeiler M, Straube WL, Lundberg E, Uhlen M, Mann M,. 2012. A protein epitope signature tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines. Mol Cell Proteomics 11: O111 009613.
155. Zhang Y, Fonslow BR, Shan B, Baek MC, Yates JR III., 2013. Protein analysis by shotgun/bottom-up proteomics. Chem Rev 113: 2343-2394.
156. Zhao H, Yannaing S, Thanthan S, Kuwayama H,. 2011. Sulfated gastrin stimulates ghrelin and growth hormone release but inhibits insulin secretion in cattle. Peptides 32: 2218-2224.
157. Zhao H, Matsuda S, Thanthan S, Yannaing S, Kuwayama H,. 2012. Bombesin-like peptides stimulate growth hormone secretion mediated by the gastrin-releasing peptide receptor in cattle. Peptides 37: 194-199.