Evaluation of interspecimen trypsin digestion efficiency prior to multiple reaction monitoring-based absolute protein quantification with native protein calibrators

Journal of Proteome Research

Volumn 12, Issue 12, 2013, Pages 5760-5774

  Van Den Broek, Irene ^a   Smit, Nico P M ^a   Romijn, Fred P H T M ^a   Van Der Laarse, Arnoud ^a   Deelder, André M ^a   Van Der Burgt, Yuri E M ^a   Cobbaert, Christa M ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

apolipoprotein; clinical chemistry; LC MS MS; metrological traceability; MRM; quantitative proteomics; trypsin digestion

Indexed keywords

APOLIPOPROTEIN A1; APOLIPOPROTEIN B; TRYPSIN; PEPTIDE FRAGMENT;

ACCURACY; ARTICLE; CALIBRATION; CLINICAL CHEMISTRY; CONTROLLED STUDY; HUMAN; HYPERTRIGLYCERIDEMIA; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MULTIPLE REACTION MONITORING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN MODIFICATION; PROTEOMICS; QUANTITATIVE ANALYSIS; TURBIDIMETRY; AMINO ACID SEQUENCE; BLOOD; CASE CONTROL STUDY; CHEMISTRY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PHOTOMETRY; REPRODUCIBILITY; STANDARD; STANDARDS;

AMINO ACID SEQUENCE; APOLIPOPROTEIN A-I; APOLIPOPROTEINS B; CALIBRATION; CASE-CONTROL STUDIES; CHROMATOGRAPHY, LIQUID; HUMANS; HYPERTRIGLYCERIDEMIA; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NEPHELOMETRY AND TURBIDIMETRY; PEPTIDE FRAGMENTS; PROTEOLYSIS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; TRYPSIN;

EID: 84890063360     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr400763d     Document Type: Article

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