Doping control analysis of seven bioactive peptides in horse plasma by liquid chromatography-mass spectrometry

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 8, 2013, Pages 2595-2606

  Kwok, Wai Him ^a   Ho, Emmie N M ^a   Lau, Ming Yip ^a   Leung, Gary N W ^a   Wong, April S Y ^a   Wan, Terence S M ^a  

^a Racing Laboratory   (Hong Kong)

Author keywords

Bioactive peptides; Doping control; Horse plasma; Liquid chromatography mass spectrometry.

Indexed keywords

BIOACTIVE PEPTIDES; DOPING CONTROL; DOPING CONTROL ANALYSIS; HIGH RESOLUTION MASS SPECTROMETRY; INSTRUMENTAL ANALYSIS; LIQUID CHROMATOGRAPHIC SEPARATIONS; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; SOLID-PHASE EXTRACTION;

ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; BIOMOLECULES; CHROMATOGRAPHY; EXTRACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLITES; PLASMAS; PURIFICATION;

PEPTIDES;

PEPTIDE;

ANIMAL; ANIMAL DISEASE; ARTICLE; BLOOD; DOPING; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HORSE; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METHODOLOGY; SOLID PHASE EXTRACTION; SUBSTANCE ABUSE;

ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DOPING IN SPORTS; HORSES; MASS SPECTROMETRY; PEPTIDES; SOLID PHASE EXTRACTION; SUBSTANCE ABUSE DETECTION;

EQUIDAE;

EID: 84878400533     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-6697-9     Document Type: Article

References (30)

1. Kuuranne T, Thomas A, Leinonen A, Delahaut P, Bosseloir A, Schänzer W, Thevis M (2008) Insulins in equine urine: qualitative analysis by immunoaffinity purification and liquid chromatography/tandem mass spectrometry for doping control purposes in horse-racing. Rapid Commun Mass Spectrom 3:355-362
2. Ho ENM, Wan TSM, Wong ASY, Lam KKH, Stewart BD (2008) Doping control analysis of insulin and its analogues in equine plasma by liquid chromatography. J Chromatogr A 1201:183-190
3. Ho ENM, Wan TSM, Wong ASY, Lam KKH, Stewart BD (2011) Doping control analysis of insulin and its analogues in equine urine by liquid chromatography-tandem mass spectrometry. J Chromatogr A 1218:1139-1146
4. Thomas A, Schänzer W, Delahaut P, Thevis M (2012) Immunoaffinity purification of peptide hormones prior to liquid chromatography-mass spectrometry in doping controls. Methods 56:230-235
5. De Kock SS, Rodgers JP, Swanepoel BC (2001) Growth hormone abuse in the horse: preliminary assessment of a mass spectrometric procedure for IGF-1 identification and quantitation. Rapid Commun Mass Spectrom 15:1191-1197
6. Popot MA, Woolfitt AR, Garcia P, Tabet JC (2008) Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry. Anal Bioanal Chem 390:1843-1852
7. Bredehöft M, Schänzer W, Thevis M (2008) Quantification of human insulin-like growth factor-1 and qualitative detection of its analogues in plasma using liquid chromatography/electrospray ionisation tandem mass spectrometry. Rapid Commun Mass Spectrom 22:477-485
8. Guan F, Uboh CE, Soma LR, Birks E (2007) LC-MS/MS method for confirmation of recombinant human erythropoietin and darbepoetin alpha in equine plasma. Anal Chem 79:4627-4635
9. Guan F, Uboh CE, Soma LR, Maylin G, Jiang Z, Chen J (2010) Confirmatory analysis of continuous erythropoietin receptor activator and erythropoietin analogues in equine plasma by LC-MS for doping control. Anal Chem 82:9074-9081
10. Yu NH, Ho ENM, Wan TSM, Wong ASY (2010) Doping control analysis of recombinant human erythropoietin, darbepoetin alfa and methoxy polyethylene glycol-epoetin beta in equine plasma by nano-liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem 396:2513-2521
11. Kwok WH, Ho ENM, Leung GNW, Wong ASY, Yue SK, Wan TSM (2012) Identification of recombinant human relaxin-2 in equine plasma by liquid chromatography/high resolution mass spectrometry. Drug Test Anal. doi: 10.1002/dta.1427
12. Okano M, Sato M, Ikekita A, Kageyama S (2010) Determination of growth hormone secretagogue pralmorelin (GHRP-2) and its metabolite in human urine by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom 24:2046-2056
13. Thomas A, Höppner S, Geyer H, Schänzer W, Petrou M, Kwiatkowska D, Pokrywka A, Thevis M (2011) Determination of growth hormone releasing peptides (GHRP) and their major metabolites in human urine for doping controls by means of liquid chromatography mass spectrometry. Anal Bioanal Chem 401:507-516
14. Thomas A, Kohler M, Schänzer W, Kamber M, Delahaut P, Thevis M (2009) Determination of Synacthen in urine for sports drug testing by means of nano-ultra-performance liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 23:2669-74
15. Esposito S, Deventer K, Goeman J, Van der Eycken J, Van Eenoo P (2012) Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential. Drug Test Anal 4:733-738
16. Ho ENM, Kwok WH, Wong ASY, Wan TSM (2012) Doping control analysis of TB-500, a synthetic version of an active region of thymosin β4, in equine urine and plasma by liquid chromatography/mass spectrometry. J Chromatogr A 1265:57-69
17. Smith RG (2005) Development of growth hormone secretagogues. Endocr Rev 26:346-360
18. Ghigo E, Arvat E, Muccioli G, Camanni F (1997) Growth hormone-releasing peptides. Eur J Endocrinol 136:445-460
19. Jetté L, Léger R, Thibaudeau K, Benquet C, Robitaille M, Pellerin I, Paradis V, van Wyk P, Pham K, Bridon DP (2005) Human growth hormone-releasing factor (hGRF)1-29-albumin bioconjugates activate the GRF receptor on the anterior pituitary in rats: identification of CJC-1295 as a long-lasting GRF analog. Endocrinology 146:3052-3058
20. Teichman SL, Neale A, Lawrence B, Gagnon C, Castaigne JP, Frohman LA (2006) Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults. J Clin Endocrinol Metab 91:799-805
21. Sackmann-Sala L, Ding J, Frohman LA, Kopchick JJ (2009) Activation of the GH/IGF-1 axis by CJC-1295, a long-acting GHRH analog, results in serum protein profile changes in normal adult subjects. Growth Horm IGF Res 19:471-477
22. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K (1999) Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 402:656-660
23. 2 stimulate growth hormone release through distinct receptor sites. J Endocrinol 129:11-19
24. Free Press Release (2011) http://www.free-press-release.com/news-do-you- know-how-to-use-tb-500-read-on-1317211858.html Assessed 24 April 2012
25. van den Broek I, Sparidans RW, Schellens JHM, Beijnen JH (2008) Quantitative bioanalysis of peptides by liquid chromatography coupled to (tandem) mass spectrometry. J Chromatogr B 872:1-22
26. Chambers E, Wagrowski-Diehl DM, Lu Z, Mazzeo JR (2007) Systematic and comprehensive strategy for reducing matrix effects in LC/MS/MS analyses. J Chromatogr B Anal Technol Biomed Life Sci 852:22-34
27. Smith EL (1949) Proteolytic Enzymes. Annu Rev Biochem 18:35-58
28. Frohman LA, Downs TR, Heimer EP, Felix AM (1989) Dipeptidylpeptidase IV and trypsin-like enzymatic degradation of human growth hormone-releasing hormone in plasma. J Clin Invest 83:1533-1540
29. Wong JKY, Tang FPW, Wan TSM (2011) In vitro metabolic studies using homogenised horse liver in place of horse liver microsomes. Drug Test Anal 3:393-399
30. Duffus JH, Nordberg M, Templeton DM (2007) Glossary of terms used in toxicology, 2nd edition. Pure Appl Chem 79:1153-1344