Rapid and Robust Polyprotein Production Facilitates Single-Molecule Mechanical Characterization of β-Barrel Assembly Machinery Polypeptide Transport Associated Domains

ACS Nano

Volumn 9, Issue 9, 2015, Pages 8811-8821

  Hoffmann, Toni ^a   Tych, Katarzyna M ^a   Crosskey, Thomas ^a   Schiffrin, Bob ^a   Brockwell, David J ^a   Dougan, Lorna ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

BamA ( barrel assembly machinery); force spectroscopy; Gibson assembly; polyprotein; POTRA (polypeptide transport associated); protein unfolding

Indexed keywords

ATOMIC FORCE MICROSCOPY; BENCHMARKING; ESCHERICHIA COLI; FRACTURE MECHANICS; MACHINERY; MECHANICAL PROPERTIES; MOLECULES; PROTEINS;

FORCE SPECTROSCOPY; MECHANICAL CHARACTERIZATIONS; NON-SPECIFIC INTERACTIONS; POLYPROTEIN; POTRA (POLYPEPTIDE TRANSPORT ASSOCIATED); PROTEIN UNFOLDING; SINGLE MOLECULE FORCE SPECTROSCOPY; SINGLE-MOLECULE STUDIES;

ASSEMBLY;

PEPTIDE; POLYPROTEIN;

ATOMIC FORCE MICROSCOPY; CHEMISTRY; ESCHERICHIA COLI; PROTEIN MULTIMERIZATION; PROTEIN TERTIARY STRUCTURE; ULTRASTRUCTURE;

ESCHERICHIA COLI; MICROSCOPY, ATOMIC FORCE; PEPTIDES; POLYPROTEINS; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, TERTIARY;

EID: 84942342619     PISSN: 19360851     EISSN: 1936086X     Source Type: Journal    
DOI: 10.1021/acsnano.5b01962     Document Type: Article

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