Biochemistry: Direct observation of the three-state folding of a single protein molecule

Science

Volumn 309, Issue 5743, 2005, Pages 2057-2060

  Cecconi, Giro ^a   Shank, Elizabeth A ^a   Bustamante, Carlos ^a   Marqusee, Susan ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; ENZYME KINETICS; ESCHERICHIA COLI; MOLECULAR ORIENTATION;

OPTICAL TWEEZERS; RIBONUCLEASE; THREE-STATE FOLDING; UNFOLDING;

PROTEINS;

RIBONUCLEASE H; DNA; ESCHERICHIA COLI PROTEIN; RIBONUCLEASE HI;

PROTEIN;

ARTICLE; COMPLIANCE (PHYSICAL); CORRELATION ANALYSIS; ENERGY; ENZYME ANALYSIS; ESCHERICHIA COLI; FORCE; FORCE MEASURING OPTICAL TWEEZERS; HYSTERESIS; MOLECULAR BIOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; TECHNICAL AID; THERMODYNAMICS; CHEMISTRY; CIRCULAR DICHROISM; OPTICS;

ESCHERICHIA COLI; HUMULUS LUPULUS;

CIRCULAR DICHROISM; DNA; ESCHERICHIA COLI PROTEINS; OPTICS; PROTEIN FOLDING; RIBONUCLEASE H, CALF THYMUS; THERMODYNAMICS;

EID: 25444512161     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1116702     Document Type: Article

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32. We thank R. Dahlquist for support and experimental advice in the early stages of this work, and the Marqusee and Bustamante labs for helpful discussions. Supported by NIH grants GM50945 (S.M.) and GM32543 (C.B.), U.S. Department of Energy grant AC0376Sf00098 (C.B.), NIH training grant GM008295 (E.A.S.), and a NSF predoctoral fellowship (E.A.S.).