Versatile cloning system for construction of multimeric proteins for use in atomic force microscopy

Protein Science

Volumn 11, Issue 9, 2002, Pages 2179-2183

  Steward, Annette ^a   Toca Herrera, José Luis ^a   Clarke, Jane ^a  

^a MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

Author keywords

AFM; Force; Linker; Mutant; Polyprotein; Titin

Indexed keywords

PROTEIN; CONNECTIN; MUSCLE PROTEIN; POLYMER; PROTEIN KINASE; PROTEIN SUBUNIT;

ARTICLE; ATOMIC FORCE MICROSCOPY; FORCE; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; TECHNIQUE; CHEMISTRY; GENE VECTOR; GENETICS; METABOLISM; METHODOLOGY; PLASMID;

CLONING, MOLECULAR; GENETIC VECTORS; MICROSCOPY, ATOMIC FORCE; MUSCLE PROTEINS; PLASMIDS; POLYMERS; PROTEIN KINASES; PROTEIN SUBUNITS; SUPPORT, NON-U.S. GOV'T;

EID: 0036707999     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0212702     Document Type: Article

References (8)

1. Can nonmechanical proteins withstand force? Stretching barnase by atomic force microscopy and molecular dynamics simulation
2. Mechanical and chemicalunfolding of a single protein: A comparison
3. Mapping the folding pathway of an immunoglobulin domain: Structural detail from phi value analysis and movement of the transition state
4. Atomic force microscopy reveals the mechanical design of a modular protein
5. Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels
6. The molecular elasticity of the extracellular matrix protein tenascin
7. Reversible unfolding of individual titin immunoglobulin domains by AFM
8. Single molecule force spectroscopy of spectrin repeats: Low unfolding forces in helix bundles