Mechanically unfolding proteins: The effect of unfolding history and the supramolecular scaffold

Protein Science

Volumn 11, Issue 12, 2002, Pages 2759-2765

  Zinober, Rebecca C ^a   Brockwell, David J ^a   Beddard, Godfrey S ^a   Blake, Anthony W ^a   Olmsted, Peter D ^a   Radford, Sheena E ^a   Alastair Smith, D ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Concatamer; I27; Mechanical resistance; Mechanical unfolding of proteins; Monte Carlo; Transition state theory; Worm like chain

Indexed keywords

MUTANT PROTEIN; POLYPROTEIN; PROTEIN;

ANALYTIC METHOD; ARTICLE; FORCE; MOLECULAR DYNAMICS; MONTE CARLO METHOD; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; METABOLISM; PROTEIN TERTIARY STRUCTURE;

COMPUTER SIMULATION; MODELS, MOLECULAR; MONTE CARLO METHOD; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTEINS;

EID: 0036891724     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0224602     Document Type: Article

References (26)

1. Bell, G.I. 1978. Models for the specific adhesion of cells to cells. Science 200: 618-627.
2. Best, R.B., Li, B., Steward, A., Daggett, V., and Clarke, J. 2001. Can non-mechanical proteins withstand force? Stretching Bamase by atomic force microscopy and molecular dynamics simulation. Biophys. J. 81: 2344-2356.
3. Best, R.B., Brockwell, D.J., Toca-Herrera, J.L., Blake, A.W., Smith, D.A., Radford, S.E., and Clarke, J. 2002a. Force mode AFM as a tool for protein folding studies. Anal. Chim. Acta (in press).
4. Best, R.B., Fowler, S.B., Toca-Herrera, J.L., Clarke, J. 2002. A simple method for probing the mechanical unfolding pathway of proteins in detail. Proc. Natl. Acad. Sci. USA. 99: 12143-12148.
5. Brockwell, D.J., Beddard, G.S., Clarkson, J., Zinober, R.C., Blake, A.W., Trinick, J., Olmsted, P.D., Smith, D.A., and Radford, S.E. 2002. The effect of core destabilisation on the mechanical resistance of I27. Biophys. J. 83: 458-472.
6. Carrion-Vazquez, M., Oberhauser, A.F., Fowler, S.B., Marszalek, P.E., Broedel, S.E., Clarke, J., and Fernandez, J.M. 1999. Mechanical and chemical unfolding of a single protein: A comparison. Proc. Natl. Acad. Sci. 96: 3694-3699.
7. Evans, E. and Ritchie, K. 1997. Dynamic strength of molecular adhesion bonds. Biophys. J. 72: 1541-1555.
8. -. 1999. Strength of a weak bond connecting flexible polymer chains. Biophys. J. 76: 2439-2447.
9. Fowler, S.B., Best, R.B., Toca-Herrera, J.L., Rutherford, T.J., Steward, A., Paci, E., Karplus, M., Clarke, J. 2002. Mechanical unfolding of a titin Ig domain: Structure of unfolding intermediate revealed by combining AFM, molecular dynamics simulations, NMR, and protein engineering. J. Mol. Biol. 322: 841.
10. Fritz, J., Katopodis, A.G., Kolbinger, F., and Anselmetti, D. 1998. Force-mediated kinetics of single P-selectin/ligand complexes observed by atomic force microscopy. Proc. Natl. Acad. Sci. 95: 12283-12288.
11. Kellermayer, M.S.Z., Smith, S.B., Granzier, H.L., and Bustamante, C. 1997. Folding-unfolding transitions in single titin molecules characterized with laser tweezers. Science 276: 1112-1116.
12. Labeit, S. and Kolmerer, B. 1995. Titins: Giant proteins in charge of muscle ultrastructure and elasticity. Science 270: 293-296.
13. Li, H., Oberhauser, A.F., Fowler, S.B., Clarke, J., and Fernandez, J.M. 2000. Atomic force microscopy reveals the mechanical design of a modular protein. Proc. Natl. Acad. Sci. 97: 6527-6531.
14. Li, H., Oberhauser, A.F., Redick, S.D., Carrion-Vazquez, M., Erickson, H.P., and Fernandez, J.M. 2001. Multiple conformations of PEVK proteins detected by single-molecule techniques, Proc. Natl. Acad. Sci. 98: 10682-10686.
15. Li, H., Linke, W.A., Oberhauser, A.F., Carrion-Vasquez, M., Kerkvliet, J.G., Lu, H., Marszatek, P.F., Fernandez, J.M. 2002. Nature 418: 998-1002.
16. Linke, W.A., Ivemeyer, M., Olivieri, N., Kolmerer, B., Casper Rüegg, J., and Labeit, S. 1996. Towards a molecular understanding of the elasticity of Titin. J. Mol. Biol. 261: 62-71.
17. Linke, W.A., Ivemeyer, M., Mundel, P., Stockmeier, M.R., and Kolmerer, B. 1998. Nature of PEVK-titin elasticity in skeletal muscle. Proc. Natl. Acad. Sci. 95: 8052-8057.
18. Makarov, D.E., Hansma, P.K., and Metiu, H. 2001. Kinetic Monte Carlo simulation of titin unfolding. J. Chem. Phys. 114: 9663-9673.
19. Merkel, R., Nassoy, P., Leung, A., Ritchie, K., and Evans, E. 1999. Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy. Nature 397: 50-53.
20. Mitsui, K., Hara, M., and Ikai, A. 1996. Mechanical unfolding of α2-macroglobulin molecules with atomic force microscope. FEBS Lett. 385: 29-33.
21. Rief, M., Gautel, M., Oesterhelt, F., Fernandez, J.M., and Gaub, H.E. 1997. Reversible unfolding of individual titin immunoglobulin domains by AFM. Science 276: 1109-1111.
22. Rief, M., Fernandez, J.M., and Gaub, H.E. 1998. Elastically coupled two-level systems as a model for biopolymer extensibility. Phys. Rev. Lett. 81: 4764-4767.
23. Rief, M., Pascual, J., Saraste, M., and Gaub, H.E. 1999. Single molecule force spectroscopy of spectrin repeats: Low unfolding forces in helix bundles. J. Mol. Biol. 286: 553-561.
24. Smith, B.L., Schäffer, T.E., Viani, M., Thompson, J.B., Frederick, N.A., Kindt, J., Belcher, A., Stucky, G.D., Morse, D.E., and Hansma, P.K. 1999. Molecular mechanistic origin of the origin of the toughness of natural adhesives, fibres and composites. Nature 399: 761-763.
25. Tskhovrebova, L., Trinick, J., Sleep, J.A., and Simmons, R.M. 1997. Elasticity and unfolding of single molecules of the giant muscle protein titin. Nature 387: 308-312.
26. Yang, G., Cecconi, C., Baase, W.A., Vetter, I.R., Breyer, W.A., Haack, J.A., Matthews, B.W., Dahlquist, F.W., and Bustamante, C. 2000. Solid-state synthesis and mechanical unfolding of polymers of T4 lysozyme. Proc. Natl. Acad. Sci. 97: 139-144.