Cantharidin induces DNA damage and inhibits DNA repair-associated protein levels in NCI-H460 human lung cancer cells

Environmental Toxicology

Volumn 30, Issue 10, 2015, Pages 1135-1143

  Hsia, Te Chun ^a,b   Lin, Ju Hwa ^a   Hsu, Shu Chun ^a   Tang, Nou Ying ^a   Lu, Hsu Feng ^c   Wu, Shin Hwar ^d   Lin, Jaung Geng ^a   Chung, Jing Gung ^a,e  

^a CHINA MEDICAL UNIVERSITY   (Taiwan)

^b CHINA MEDICAL UNIVERSITY HOSPITAL   (Taiwan)

^c CHENG HSIN GENERAL HOSPITAL   (Taiwan)

^d CHANGHUA CHRISTIAN HOSPITAL   (Taiwan)

^e ASIA UNIVERSITY   (Taiwan)

Author keywords

Cantharidin; Comet assay; DNA damage; DNA repair; Human lung cancer NCI H460 cells

Indexed keywords

AMINO ACIDS; BIOLOGICAL ORGANS; CELL DEATH; CONDENSATION; CYTOLOGY; CYTOTOXICITY; DISEASES; DNA; PROTEINS; REPAIR; RESPIRATORY SYSTEM;

CANTHARIDIN; COMET ASSAYS; DNA DAMAGES; DNA REPAIR; HUMAN LUNG;

CELLS;

ATM PROTEIN; BRCA1 PROTEIN; CANTHARIDIN; HISTONE H2AX; MEDIATOR OF DNA DAMAGE CHECKPOINT PROTEIN 1; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE; PROTEIN; PROTEIN 14 3 3; PROTEIN P53; PROTEIN SERINE THREONINE KINASE; UNCLASSIFIED DRUG; DNA LIGASE;

ARTICLE; CELL DEATH; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOPLASM; DNA DAMAGE; DOSE RESPONSE; HUMAN; HUMAN CELL; IN VITRO STUDY; LUNG CANCER CELL LINE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; ANTAGONISTS AND INHIBITORS; APOPTOSIS; CONFOCAL MICROSCOPY; DNA REPAIR; DRUG EFFECTS; LUNG TUMOR; METABOLISM; PATHOLOGY; TUMOR CELL LINE;

MYLABRIS;

APOPTOSIS; CANTHARIDIN; CELL LINE, TUMOR; COMET ASSAY; DNA DAMAGE; DNA REPAIR; DNA REPAIR ENZYMES; HUMANS; LUNG NEOPLASMS; MICROSCOPY, CONFOCAL;

EID: 84941179469     PISSN: 15204081     EISSN: 15227278     Source Type: Journal    
DOI: 10.1002/tox.21986     Document Type: Article

References (44)

1. Ashby J, Tinwell H, Lefevre PA, Browne MA. 1995. The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment. Mutagenesis 10:85-90.
2. Bassing CH, Chua KF, Sekiguchi J, Suh H, Whitlow SR, Fleming JC, Monroe BC, Ciccone DN, Yan C, Vlasakova K, Livingston DM, Ferguson DO, Scully R, Alt FW. 2002. Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX. Proc Natl Acad Sci U S A 99:8173-8178.
3. Bishayee K, Paul A, Ghosh S, Sikdar S, Mukherjee A, Biswas R, Boujedaini N, Khuda-Bukhsh AR. 2013. Condurango-glycoside-A fraction of Gonolobus condurango induces DNA damage associated senescence and apoptosis via ROS-dependent p53 signalling pathway in HeLa cells. Mol Cell Biochem 382:173-183.
4. Bostancioglu RB, Demirel S, Turgut Cin G, Koparal AT. 2013. Novel ferrocenyl-containing N-acetyl-2-pyrazolines inhibit in vitro angiogenesis and human lung cancer growth by interfering with F-actin stress fiber polimeryzation. Drug Chem Toxicol 36:484-495.
5. Celeste A, Fernandez-Capetillo O, Kruhlak MJ, Pilch DR, Staudt DW, Lee A, Bonner RF, Bonner WM, Nussenzweig A. 2003. Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks. Nat Cell Biol 5:675-679.
6. Celeste A, Petersen S, Romanienko PJ, Fernandez-Capetillo O, Chen HT, Sedelnikova OA, Reina-San-Martin B, Coppola V, Meffre E, Difilippantonio MJ, Redon C, Pilch DR, Olaru A, Eckhaus M, Camerini-Otero RD, Tessarollo L, Livak F, Manova K, Bonner WM, Nussenzweig MC, Nussenzweig A. 2002. Genomic instability in mice lacking histone H2AX. Science 296:922-927.
7. Chiang JH, Yang JS, Lu CC, Hour MJ, Chang SJ, Lee TH, Chung JG. 2013. Newly synthesized quinazolinone HMJ-38 suppresses angiogenetic responses and triggers human umbilical vein endothelial cell apoptosis through p53-modulated Fas/death receptor signaling. Toxicol Appl Pharmacol 269:150-162.
8. Chiu TH, Lan KY, Yang MD, Lin JJ, Hsia TC, Wu CT, Yang JS, Chueh FS, Chung JG. 2013. Diallyl sulfide promotes cell-cycle arrest through the p53 expression and triggers induction of apoptosis via caspase- and mitochondria-dependent signaling pathways in human cervical cancer Ca Ski cells. Nutr Cancer 65:505-514.
9. Choi JH, Sancar A, Lindsey-Boltz LA. 2009. The human ATR-mediated DNA damage checkpoint in a reconstituted system. Methods 48:3-7.
10. Coloe Dosal J, Stewart PW, Lin JA, Williams CS, Morrell DS. 2012. Cantharidin for the treatment of molluscum contagiosum: A prospective, double-blinded, placebo-controlled trial. Pediatr Dermatol, Aug 16. doi: 10.1111/j.1525-1470.2012.01810.x. [Epub ahead of print]
11. De Luca P, Moiola CP, Zalazar F, Gardner K, Vazquez ES, De Siervi A. 2013. BRCA1 and p53 regulate critical prostate cancer pathways. Prostate Cancer Prostatic Dis 16:233-238.
12. Dejmek J, Iglehart JD, Lazaro JB. 2009. DNA-dependent protein kinase (DNA-PK)-dependent cisplatin-induced loss of nucleolar facilitator of chromatin transcription (FACT) and regulation of cisplatin sensitivity by DNA-PK and FACT. Mol Cancer Res 7:581-591.
13. Donatus IA, Sardjoko, Vermeulen NP. 1990. Cytotoxic and cytoprotective activities of curcumin. Effects on paracetamol-induced cytotoxicity, lipid peroxidation and glutathione depletion in rat hepatocytes. Biochem Pharmacol 39:1869-1875.
14. Dumenco LL, Allay E, Norton K, Gerson SL. 1993. The prevention of thymic lymphomas in transgenic mice by human O6-alkylguanine-DNA alkyltransferase. Science 259:219-222.
15. Friedman HS, McLendon RE, Kerby T, Dugan M, Bigner SH, Henry AJ, Ashley DM, Krischer J, Lovell S, Rasheed K, Marchev F, Seman AJ, Cokgor I, Rich J, Stewart E, Colvin OM, Provenzale JM, Bigner DD, Haglund MM, Friedman AH, Modrich PL. 1998. DNA mismatch repair and O6-alkylguanine-DNA alkyltransferase analysis and response to Temodal in newly diagnosed malignant glioma. J Clin Oncol 16:3851-3857.
16. Graziano MJ, Pessah IN, Matsuzawa M, Casida JE. 1988. Partial characterization of specific cantharidin binding sites in mouse tissues. Mol Pharmacol 33:706-712.
17. Hegi ME, Diserens AC, Gorlia T, Hamou MF, de Tribolet N, Weller M, Kros JM, Hainfellner JA, Mason W, Mariani L, Bromberg JE, Hau P, Mirimanoff RO, Cairncross JG, Janzer RC and Stupp R. 2005. MGMT gene silencing and benefit from temozolomide in glioblastoma. N Engl J Med 352:997-1003.
18. Huan SK, Lee HH, Liu DZ, Wu CC, Wang CC. 2006. Cantharidin-induced cytotoxicity and cyclooxygenase 2 expression in human bladder carcinoma cell line. Toxicology 223:136-143.
19. Huan SK, Wang KT, Yeh SD, Lee CJ, Lin LC, Liu DZ, Wang CC. 2012. Scutellaria baicalensis alleviates cantharidin-induced rat hemorrhagic cystitis through inhibition of cyclooxygenase-2 overexpression. Molecules 17:6277-6289.
20. Huang WW, Ko SW, Tsai HY, Chung JG, Chiang JH, Chen KT, Chen YC, Chen HY, Chen YF, Yang JS. 2011. Cantharidin induces G2/M phase arrest and apoptosis in human colorectal cancer colo 205 cells through inhibition of CDK1 activity and caspase-dependent signaling pathways. Int J Oncol 38:1067-1073.
21. Hundt HK, Steyn JM, Wagner L. 1990. Post-mortem serum concentration of cantharidin in a fatal case of cantharides poisoning. Hum Exp Toxicol 9:35-40.
22. Jesien-Lewandowicz E1, Jesionek-Kupnicka D, Zawlik I, Szybka M, Kulczycka-Wojdala D, Rieske P, Sieruta M, Jaskolski D, Och W, Skowronski W, Sikorska B, Potemski P, Papierz W, Liberski PP and Kordek R. 2009. High incidence of MGMT promoter methylation in primary glioblastomas without correlation with TP53 gene mutations. Cancer Genet Cytogenet 188:77-82.
23. Ji BC, Yu CC, Yang ST, Hsia TC, Yang JS, Lai KC, Ko YC, Lin JJ, Lai TY, Chung JG. 2012. Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells. Oncol Rep 27:959-964.
24. Kuo JH, Chu YL, Yang JS, Lin JP, Lai KC, Kuo HM, Hsia TC, Chung JG. 2010. Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways. Int J Oncol 37:1243-1250.
25. Li W, Xie L, Chen Z, Zhu Y, Sun Y, Miao Y, Xu Z, Han X. 2010. Cantharidin, a potent and selective PP2A inhibitor, induces an oxidative stress-independent growth inhibition of pancreatic cancer cells through G2/M cell-cycle arrest and apoptosis. Cancer Sci 101:1226-1233.
26. Li YM, Mackintosh C, Casida JE. 1993. Protein phosphatase 2A and its [3H]cantharidin/[3H]endothall thioanhydride binding site. Inhibitor specificity of cantharidin and ATP analogues. Biochem Pharmacol 46:1435-1443.
27. Liu B, Gao HC, Xu JW, Cao H, Fang XD, Gao HM, Qiao SX. 2012. Apoptosis of colorectal cancer UTC116 cells induced by Cantharidinate. Asian Pac J Cancer Prev 13:3705-3708.
28. Lu HF, Yang JS, Lai KC, Hsu SC, Hsueh SC, Chen YL, Chiang JH, Lu CC, Lo C, Yang MD, Chung JG. 2009. Curcumin-induced DNA damage and inhibited DNA repair genes expressions in mouse-rat hybrid retina ganglion cells (N18). Neurochem Res 34:1491-1497.
29. Meyer B, Voss KO, Tobias F, Jakob B, Durante M, Taucher-Scholz G. 2013. Clustered DNA damage induces pan-nuclear H2AX phosphorylation mediated by ATM and DNA-PK. Nucleic Acids Res 41:6109-6118.
30. Neal CL, Yao J, Yang W, Zhou X, Nguyen NT, Lu J, Danes CG, Guo H, Lan KH, Ensor J, Hittelman W, Hung MC, Yu D. 2009. 14-3-3zeta overexpression defines high risk for breast cancer recurrence and promotes cancer cell survival. Cancer Res 69:3425-3432.
31. Olive PL, Banath JP, Durand RE. 1990. Detection of etoposide resistance by measuring DNA damage in individual Chinese hamster cells. J Natl Cancer Inst 82:779-783.
32. Pool-Zobel BL, Lotzmann N, Knoll M, Kuchenmeister F, Lambertz R, Leucht U, Schroder HG, Schmezer P. 1994. Detection of genotoxic effects in human gastric and nasal mucosa cells isolated from biopsy samples. Environ Mol Mutagen 24:23-45.
33. Sagawa M, Nakazato T, Uchida H, Ikeda Y, Kizaki M. 2008. Cantharidin induces apoptosis of human multiple myeloma cells via inhibition of the JAK/STAT pathway. Cancer Sci 99:1820-1826.
34. Shiotani B, Zou L. 2009. Single-stranded DNA orchestrates an ATM-to-ATR switch at DNA breaks. Mol Cell 33:547-558.
35. Shou LM, Zhang QY, Li W, Xie X, Chen K, Lian L, Li ZY, Gong FR, Dai KS, Mao YX and Tao M. 2013. Cantharidin and norcantharidin inhibit the ability of MCF-7 cells to adhere to platelets via protein kinase C pathway-dependent downregulation of alpha2 integrin. Oncol Rep 30:1059-1066.
36. Tice RR, Andrews PW, Singh NP. 1990. The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair. Basic Life Sci 53:291-301.
37. Venkitaraman AR. 2002. Cancer susceptibility and the functions of BRCA1 and BRCA2. Cell 108:171-182.
38. Wang CC, Wu CH, Hsieh KJ, Yen KY, Yang LL. 2000. Cytotoxic effects of cantharidin on the growth of normal and carcinoma cells. Toxicology 147:77-87.
39. Wong J, Phelps R, Levitt J. 2012. Treatment of acquired perforating dermatosis with cantharidin. Arch Dermatol 148:160-162.
40. Wu CL, Huang AC, Yang JS, Liao CL, Lu HF, Chou ST, Ma CY, Hsia TC, Ko YC, Chung JG. 2011. Benzyl isothiocyanate (BITC) and phenethyl isothiocyanate (PEITC)-mediated generation of reactive oxygen species causes cell cycle arrest and induces apoptosis via activation of caspase-3, mitochondria dysfunction and nitric oxide (NO) in human osteogenic sarcoma U-2 OS cells. J Orthop Res 29:1199-1209.
41. Xu X, Gammon MD, Zhang Y, Bestor TH, Zeisel SH, Wetmur JG, Wallenstein S, Bradshaw PT, Garbowski G, Teitelbaum SL, Neugut AI, Santella RM and Chen J. 2009. BRCA1 promoter methylation is associated with increased mortality among women with breast cancer. Breast Cancer Res Treat 115:397-404.
42. Yang JS, Hour MJ, Huang WW, Lin KL, Kuo SC, Chung JG. 2010. MJ-29 inhibits tubulin polymerization, induces mitotic arrest, and triggers apoptosis via cyclin-dependent kinase 1-mediated Bcl-2 phosphorylation in human leukemia U937 cells. J Pharmacol Exp Ther 334:477-488.
43. Zaidi NH, Pretlow TP, O'Riordan MA, Dumenco LL, Allay E, Gerson SL. 1995. Transgenic expression of human MGMT protects against azoxymethane-induced aberrant crypt foci and G to A mutations in the K-ras oncogene of mouse colon. Carcinogenesis 16:451-456.
44. Zhang YH, Chen X. 1985. [Effect of disodium cantharidate and injectable herbal medicine on energy and cyclic nucleotide metabolism in hepatoma 22 cells and liver tissues of tumor-bearing mice]. Zhong Xi Yi Jie He Za Zhi 5:686-690, 645.