Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks

Nature Cell Biology

Volumn 5, Issue 7, 2003, Pages 675-679

  Celeste, Arkady ^a   Fernandez Capetillo, Oscar ^a   Kruhlak, Michael J ^a   Pilch, Duane R ^a   Staudt, David W ^a   Lee, Alicia ^a   Bonner, Robert F ^b   Bonner, William M ^a   Nussenzweig, André ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; BRCA1 PROTEIN; DNA; DOUBLE STRANDED DNA; GENE PRODUCT; HISTONE H2A; MUTANT PROTEIN; PROTEIN P53; TUMOR SUPPRESSOR PROTEIN;

ARTICLE; CHROMATIN; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; DNA STRAND BREAKAGE; HUMAN; HUMAN CELL; IRRADIATION; MICROENVIRONMENT; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN DEFICIENCY; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; REGULATORY MECHANISM; SIGNAL TRANSDUCTION;

ANIMALS; CELL LINE; CELL NUCLEUS; CHROMATIN; DNA DAMAGE; DNA REPAIR; DNA-BINDING PROTEINS; EUKARYOTIC CELLS; HISTONES; HUMANS; MICE; NUCLEAR PROTEINS; PHOSPHORYLATION; SIGNAL TRANSDUCTION;

EID: 0037711771     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb1004     Document Type: Article

References (27)

1. Rouse, J. & Jackson, S. P. Interfaces between the detection, signaling, and repair of DNA damage. Science 297, 547-551 (2002).
2. Nelms, B. E., Maser, R. S., MacKay, J. F., Lagally, M. G. & Petrini, J. H. In situ visualization of DNA double-strand break repair in human fibroblasts. Science 280, 590-592 (1998).
3. Paull, T. T. et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr. Biol. 10, 886-895 (2000).
4. Bassing, C. H. et al. Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX. Proc. Natl Acad. Sci. USA 99, 8173-8178 (2002).
5. Celeste, A. et al. Genomic instability in mice lacking histone H2AX. Science 296, 922-927 (2002).
6. Maser, R. S., Monsen, K. J., Nelms, B. E. & Petrini, J. H. hMre11 and hRad50 nuclear foci are induced during the normal cellular response to DNA double-strand breaks. Mol. Cell Biol. 17, 6087-6096 (1997).
7. Kobayashi, J. et al. NBS1 Localizes to γ-H2AX Foci through Interaction with the FHA/BRCT Domain. Curr. Biol. 12, 1846-1851 (2002).
8. Rappold, I., Iwabuchi, K., Date, T. & Chen, J. Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways. J. Cell Biol. 153, 613-620 (2001).
9. Tauchi, H., Matsuura, S., Kobayashi, J., Sakamoto, S. & Komatsu, K. Nijmegen breakage syndrome gene, NBS1, and molecular links to factors for genome stability. Oncogene 21, 8967-8980 (2002).
10. Rogakou, E. P., Boon, C., Redon, C. & Bonner, W. M. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J. Cell Biol. 146, 905-916 (1999).
11. Maser, R. S. et al. Mre11 complex and DNA replication: linkage to E2F and sites of DNA synthesis. Mol. Cell Biol. 21, 6006-6016 (2001).
12. Mirzoeva, O. K. & Petrini, J. H. DNA damage-dependent nuclear dynamics of the mre11 complex. Mol. Cell Biol. 21, 281-288 (2001).
13. Schultz, L. B., Chehab, N. H., Malikzay, A. & Halazonetis, T. D. p53 binding protein 1 (53BP1) is an early participant in the cellular response to DNA double-strand breaks. J. Cell Biol. 151, 1381-1390 (2000).
14. Downs, J. A., Lowndes, N. F. & Jackson, S. P. A role for Saccharomyces cerevisiae histone H2A in DNA repair. Nature 408, 1001-1004 (2000).
15. Burma, S., Chen, B. P., Murphy, M., Kurimasa, A. & Chen, D. J. ATM phosphorylates histone H2AX in response to DNA double-strand breaks. J. Biol. Chem. 276, 42462-42467 (2001).
16. Wang, J. Y. Cancer: New link in a web of human genes. Nature 405, 404-405 (2000).
17. Mirzoeva, O. K. & Petrini, J. H. DNA replication-dependent nuclear dynamics of the mre11 complex. Mol. Cancer Res. 1, 207-218 (2003).
18. Jenuwein, T. & Allis, C. D. Translating the histone code. Science 293, 1074-1080 (2001).
19. Rogakou, E. P., Pilch, D. R., Orr, A. H., Ivanova, V. S. & Bonner, W. M. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J. Biol. Chem. 273, 5858-5868 (1998).
20. Zou, L., Cortez, D. & Elledge, S. J. Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin. Genes Dev. 16, 198-208 (2002).
21. Andegeko, Y. et al. Nuclear retention of ATM at sites of DNA double strand breaks. J. Biol. Chem. 276, 38224-38230 (2001).
22. Paull, T. T. & Gellert, M. The 3′ to 5′ exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks. Mol. Cell 1, 969-979 (1998).
23. Paull, T. T., Cortez, D., Bowers, B., Elledge, S. J. & Gellert, M. Direct DNA binding by Brca1. Proc. Natl Acad. Sci. USA 98, 6086-6091 (2001).
24. van Gent, D. C., Hoeijmakers, J. H. & Kanaar, R. Chromosomal stability and the DNA double-stranded break connection. Nature Rev. Genet. 2, 196-206 (2001).
25. Khanna, K. K. & Jackson, S. P. DNA double-strand breaks: signaling, repair and the cancer connection. Nature Genet. 27, 247-254 (2001).
26. Fernandez-Capetillo, O. et al. DNA damage-induced G2-M checkpoint activation by histone H2AX and 53BP1. Nature Cell Biol. 4, 993-997 (2002).
27. Yagi, H. et al. Regulation of the mouse histone H2A.X gene promoter by the transcription factor E2F and CCAAT binding protein. J. Biol. Chem. 270, 18759-18765 (1995).