메뉴 건너뛰기




Volumn 360, Issue 1, 2015, Pages 29-42

How to count cells: the advantages and disadvantages of the isotropic fractionator compared with stereology

Author keywords

Brain size; Isotropic fractionator; NeuN; Numbers of neurons; Stereology

Indexed keywords

ANIMAL; CELL COUNT; CELL FRACTIONATION; COMPARATIVE STUDY; DEVICES; FLOW CYTOMETRY; HUMAN; PROCEDURES; RAT; TIME FACTOR;

EID: 84939968296     PISSN: 0302766X     EISSN: 14320878     Source Type: Journal    
DOI: 10.1007/s00441-015-2127-6     Document Type: Review
Times cited : (76)

References (63)
  • 4
    • 84889645520 scopus 로고    scopus 로고
    • Validation of the isotropic fractionator: comparison with unbiased stereology and DNA extraction for quantification of glial cells
    • Bahney J, Bartheld CS von (2014) Validation of the isotropic fractionator: comparison with unbiased stereology and DNA extraction for quantification of glial cells. J Neurosci Methods 222:165–174
    • (2014) J Neurosci Methods , vol.222 , pp. 165-174
    • Bahney, J.1    von, B.C.S.2
  • 5
    • 69549092121 scopus 로고    scopus 로고
    • Changing numbers of neuronal and non-neuronal cells underlie postnatal brain growth in the rat
    • COI: 1:CAS:528:DC%2BD1MXhtFWksLrJ, PID: 19666520
    • Bandeira F, Lent R, Herculano-Houzel S (2009) Changing numbers of neuronal and non-neuronal cells underlie postnatal brain growth in the rat. Proc Natl Acad Sci U S A 106:14108–14113
    • (2009) Proc Natl Acad Sci U S A , vol.106 , pp. 14108-14113
    • Bandeira, F.1    Lent, R.2    Herculano-Houzel, S.3
  • 6
    • 0035451054 scopus 로고    scopus 로고
    • Comparison of 2-D and 3-D counting: the need for calibration and common sense
    • Bartheld CS von (2001) Comparison of 2-D and 3-D counting: the need for calibration and common sense. Trends Neurosci 24:504–506
    • (2001) Trends Neurosci , vol.24 , pp. 504-506
    • von, B.C.S.1
  • 7
    • 0036108605 scopus 로고    scopus 로고
    • Counting particles in tissue sections: choices of methods and importance of calibration to minimize biases
    • Bartheld C von (2002) Counting particles in tissue sections: choices of methods and importance of calibration to minimize biases. Histol Histopathol 17:639–648
    • (2002) Histol Histopathol , vol.17 , pp. 639-648
  • 9
    • 84869861999 scopus 로고    scopus 로고
    • The isotropic fractionator provides evidence for differential loss of hippocampal neurons in two mouse models of Alzheimer’s disease
    • COI: 1:CAS:528:DC%2BC3sXitVynu7Y%3D, PID: 23173713
    • Brautigam H, Steele JW, Westaway D, Fraser PE, StGeorge-Hyslop PH, Gandy S, Hof PR, Dickstein DL (2012) The isotropic fractionator provides evidence for differential loss of hippocampal neurons in two mouse models of Alzheimer’s disease. Mol Neurodegener 7:58
    • (2012) Mol Neurodegener , vol.7 , pp. 58
    • Brautigam, H.1    Steele, J.W.2    Westaway, D.3    Fraser, P.E.4    StGeorge-Hyslop, P.H.5    Gandy, S.6    Hof, P.R.7    Dickstein, D.L.8
  • 11
    • 84872837369 scopus 로고    scopus 로고
    • Structural uniformity of neocortex, revisited
    • COI: 1:CAS:528:DC%2BC3sXhvFKrsr4%3D, PID: 23297199
    • Carlo CN, Stevens CF (2013) Structural uniformity of neocortex, revisited. Proc Natl Acad Sci U S A 110:1488–1493
    • (2013) Proc Natl Acad Sci U S A , vol.110 , pp. 1488-1493
    • Carlo, C.N.1    Stevens, C.F.2
  • 12
    • 84922389585 scopus 로고    scopus 로고
    • Systematic, cross-cortex variation in neuron numbers in rodents and primates
    • PID: 23960207
    • Charvet CJ, Cahalane DJ, Finlay BL (2015) Systematic, cross-cortex variation in neuron numbers in rodents and primates. Cereb Cortex 25:147–160
    • (2015) Cereb Cortex , vol.25 , pp. 147-160
    • Charvet, C.J.1    Cahalane, D.J.2    Finlay, B.L.3
  • 13
    • 0030027461 scopus 로고    scopus 로고
    • Methods for determining numbers of cells and synapses: a case for more uniform standards of review
    • COI: 1:STN:280:DyaK28zotFamuw%3D%3D, PID: 8789272
    • Coggeshall RE, Lekan HA (1996) Methods for determining numbers of cells and synapses: a case for more uniform standards of review. J Comp Neurol 364:6–15
    • (1996) J Comp Neurol , vol.364 , pp. 6-15
    • Coggeshall, R.E.1    Lekan, H.A.2
  • 14
    • 0021682618 scopus 로고
    • An empirical method for converting nucleolar counts to neuronal numbers
    • COI: 1:STN:280:DyaL2M7js1agtw%3D%3D, PID: 6527551
    • Coggeshall RE, Chung K, Greenwood D, Hulsebosch CE (1984) An empirical method for converting nucleolar counts to neuronal numbers. J Neurosci Methods 12:125–132
    • (1984) J Neurosci Methods , vol.12 , pp. 125-132
    • Coggeshall, R.E.1    Chung, K.2    Greenwood, D.3    Hulsebosch, C.E.4
  • 15
    • 0025600322 scopus 로고
    • Calibration of methods for determining numbers of dorsal root ganglion cells
    • COI: 1:STN:280:DyaK3M7psVejug%3D%3D, PID: 2084389
    • Coggeshall RE, La Forte R, Klein CM (1990) Calibration of methods for determining numbers of dorsal root ganglion cells. J Neurosci Methods 35:187–194
    • (1990) J Neurosci Methods , vol.35 , pp. 187-194
    • Coggeshall, R.E.1    La Forte, R.2    Klein, C.M.3
  • 16
    • 77957684979 scopus 로고    scopus 로고
    • Neuron densities vary across and within cortical areas in primates
    • COI: 1:CAS:528:DC%2BC3cXhtFOqtLfM, PID: 20798050
    • Collins CE, Airey DC, Young NA, Leitch DB, Kaas JH (2010a) Neuron densities vary across and within cortical areas in primates. Proc Natl Acad Sci U S A 107:15927–15932
    • (2010) Proc Natl Acad Sci U S A , vol.107 , pp. 15927-15932
    • Collins, C.E.1    Airey, D.C.2    Young, N.A.3    Leitch, D.B.4    Kaas, J.H.5
  • 17
    • 77951676854 scopus 로고    scopus 로고
    • A rapid and reliable method of counting neurons and other cells in brain tissue: a comparison of flow cytometry and manual counting methods
    • Collins CE, Young NA, Flaherty DK, Airey DC, Kaas JH (2010b) A rapid and reliable method of counting neurons and other cells in brain tissue: a comparison of flow cytometry and manual counting methods. Front Neuroanat 9:5
    • (2010) Front Neuroanat , vol.9 , pp. 5
    • Collins, C.E.1    Young, N.A.2    Flaherty, D.K.3    Airey, D.C.4    Kaas, J.H.5
  • 18
    • 0015886330 scopus 로고
    • Quantitative growth and development of human brain
    • COI: 1:CAS:528:DyaE2cXhtFylurw%3D, PID: 4796010
    • Dobbing J, Sands J (1973) Quantitative growth and development of human brain. Arch Dis Child 48:757–767
    • (1973) Arch Dis Child , vol.48 , pp. 757-767
    • Dobbing, J.1    Sands, J.2
  • 19
    • 1942484894 scopus 로고    scopus 로고
    • Stereological analysis of the mediodorsal thalamic nucleus in schizophrenia: volume, neuron number, and cell types
    • PID: 15065119
    • Dorph-Petersen KA, Pierri JN, Sun Z, Sampson AR, Lewis DA (2004) Stereological analysis of the mediodorsal thalamic nucleus in schizophrenia: volume, neuron number, and cell types. J Comp Neurol 472:449–462
    • (2004) J Comp Neurol , vol.472 , pp. 449-462
    • Dorph-Petersen, K.A.1    Pierri, J.N.2    Sun, Z.3    Sampson, A.R.4    Lewis, D.A.5
  • 20
    • 65649137337 scopus 로고    scopus 로고
    • Volume and neuron number of the lateral geniculate nucleus in schizophrenia and mood disorders
    • PID: 18642008
    • Dorph-Petersen KA, Caric D, Saghafi R, Zhang W, Sampson AR, Lewis DA (2009) Volume and neuron number of the lateral geniculate nucleus in schizophrenia and mood disorders. Acta Neuropathol 117:369–384
    • (2009) Acta Neuropathol , vol.117 , pp. 369-384
    • Dorph-Petersen, K.A.1    Caric, D.2    Saghafi, R.3    Zhang, W.4    Sampson, A.R.5    Lewis, D.A.6
  • 21
    • 84939981585 scopus 로고    scopus 로고
    • Update on stereology for light microscopy, Cell Tissue Res [current issue]:
    • Geuna S, Herrera-Rincon (2015) Update on stereology for light microscopy. Cell Tissue Res [current issue]
    • (2015) Herrera-Rincon
    • Geuna, S.1
  • 22
    • 0037140798 scopus 로고    scopus 로고
    • On counting and counting errors
    • COI: 1:STN:280:DC%2BD383ot1Olug%3D%3D, PID: 11967890
    • Guillery RW (2002) On counting and counting errors. J Comp Neurol 447:1–7
    • (2002) J Comp Neurol , vol.447 , pp. 1-7
    • Guillery, R.W.1
  • 23
    • 0031571766 scopus 로고    scopus 로고
    • Quantification without pontification: choosing a method for counting objects in sectioned tissues
    • COI: 1:STN:280:DyaK2svkslOrsg%3D%3D, PID: 9303520
    • Guillery RW, Herrup K (1997) Quantification without pontification: choosing a method for counting objects in sectioned tissues. J Comp Neurol 386:2–7
    • (1997) J Comp Neurol , vol.386 , pp. 2-7
    • Guillery, R.W.1    Herrup, K.2
  • 24
    • 84985187820 scopus 로고
    • Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson
    • COI: 1:STN:280:DyaL2s%2FhtFKrsw%3D%3D, PID: 3761363
    • Gundersen HJ (1986) Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J Microsc 143:3–45
    • (1986) J Microsc , vol.143 , pp. 3-45
    • Gundersen, H.J.1
  • 26
    • 0026043845 scopus 로고
    • The posterior nervous system of the nematode Caenorhabditis elegans: serial reconstruction of identified neurons and complete pattern of synaptic interactions
    • COI: 1:STN:280:DyaK3M%2FptlOgtg%3D%3D, PID: 1986064
    • Hall DH, Russell RL (1991) The posterior nervous system of the nematode Caenorhabditis elegans: serial reconstruction of identified neurons and complete pattern of synaptic interactions. J Neurosci 11:1–22
    • (1991) J Neurosci , vol.11 , pp. 1-22
    • Hall, D.H.1    Russell, R.L.2
  • 27
    • 0033612565 scopus 로고    scopus 로고
    • Analysis of cell death in the trochlear nucleus of the chick embryo: calibration of the optical disector counting method reveals systematic bias
    • Hatton WJ, Bartheld CS von (1999) Analysis of cell death in the trochlear nucleus of the chick embryo: calibration of the optical disector counting method reveals systematic bias. J Comp Neurol 409:169–186
    • (1999) J Comp Neurol , vol.409 , pp. 169-186
    • Hatton, W.J.1    von, B.C.S.2
  • 28
    • 63549138918 scopus 로고
    • Desoxyribonucleic acid content and cell density in brain and human brain tumors
    • COI: 1:CAS:528:DyaG2cXntlCnsg%3D%3D, PID: 13190467
    • Heller IH, Elliott KA (1954) Desoxyribonucleic acid content and cell density in brain and human brain tumors. Can J Biochem Physiol 32:584–592
    • (1954) Can J Biochem Physiol , vol.32 , pp. 584-592
    • Heller, I.H.1    Elliott, K.A.2
  • 29
    • 84859740567 scopus 로고    scopus 로고
    • The isotropic fractionator: a fast, reliable method to determine numbers of cells in the brain or other tissues
    • Fellin T, Halassa MM, (eds), 67, Humana, New York:
    • Herculano-Houzel S (2012) The isotropic fractionator: a fast, reliable method to determine numbers of cells in the brain or other tissues. In: Fellin T, Halassa MM (eds) Springer neuromethods: neuronal network analysis: concepts and experimental approaches, vol 67. Humana, New York, pp 391–403
    • (2012) Springer neuromethods: neuronal network analysis: concepts and experimental approaches , pp. 391-403
    • Herculano-Houzel, S.1
  • 30
    • 79951853770 scopus 로고    scopus 로고
    • Gorilla and orangutan brains conform to the primate cellular scaling rules: implications for human evolution
    • PID: 21228547
    • Herculano-Houzel S, Kaas JH (2011) Gorilla and orangutan brains conform to the primate cellular scaling rules: implications for human evolution. Brain Behav Evol 77:33–44
    • (2011) Brain Behav Evol , vol.77 , pp. 33-44
    • Herculano-Houzel, S.1    Kaas, J.H.2
  • 31
    • 14944342993 scopus 로고    scopus 로고
    • Isotropic fractionator: a simple, rapid method for the quantification of total cell and neuron numbers in the brain
    • COI: 1:CAS:528:DC%2BD2MXis1ygsrY%3D, PID: 15758160
    • Herculano-Houzel S, Lent R (2005) Isotropic fractionator: a simple, rapid method for the quantification of total cell and neuron numbers in the brain. J Neurosci 25:2518–2521
    • (2005) J Neurosci , vol.25 , pp. 2518-2521
    • Herculano-Houzel, S.1    Lent, R.2
  • 32
    • 84887033182 scopus 로고    scopus 로고
    • Distribution of neurons in functional areas of the mouse cerebral cortex reveals quantitatively different cortical zones
    • PID: 24155697
    • Herculano-Houzel S, Watson C, Paxinos G (2013) Distribution of neurons in functional areas of the mouse cerebral cortex reveals quantitatively different cortical zones. Front Neuroanat 7:35
    • (2013) Front Neuroanat , vol.7 , pp. 35
    • Herculano-Houzel, S.1    Watson, C.2    Paxinos, G.3
  • 34
    • 0015087796 scopus 로고
    • DNA and RNA and the cytoarchitecture of human frontal cortex
    • COI: 1:CAS:528:DyaE3MXkslenuro%3D, PID: 5118884
    • Hess HH, Thalheimer C (1971) DNA and RNA and the cytoarchitecture of human frontal cortex. J Neurochem 18:1281–1290
    • (1971) J Neurochem , vol.18 , pp. 1281-1290
    • Hess, H.H.1    Thalheimer, C.2
  • 36
    • 77249177035 scopus 로고    scopus 로고
    • Calibration of the stereological estimation of the number of myelinated axons in the rat sciatic nerve: a multicenter study
    • Kaplan S, Geuna S, Ronchi G, Ulkay MB, Bartheld CS von (2010) Calibration of the stereological estimation of the number of myelinated axons in the rat sciatic nerve: a multicenter study. J Neurosci Methods 187:90–99
    • (2010) J Neurosci Methods , vol.187 , pp. 90-99
    • Kaplan, S.1    Geuna, S.2    Ronchi, G.3    Ulkay, M.B.4    von, B.C.S.5
  • 37
    • 0027195099 scopus 로고
    • Total numbers of various cell types in rat cerebellar cortex estimated using an unbiased stereological method
    • COI: 1:STN:280:DyaK3s3osVCjug%3D%3D, PID: 8508308
    • Korbo L, Andersen BB, Ladefoged O, Møller A (1993) Total numbers of various cell types in rat cerebellar cortex estimated using an unbiased stereological method. Brain Res 609:262–268
    • (1993) Brain Res , vol.609 , pp. 262-268
    • Korbo, L.1    Andersen, B.B.2    Ladefoged, O.3    Møller, A.4
  • 38
    • 0025809713 scopus 로고
    • Accurate prediction of Purkinje cell number from cerebellar weight can be achieved with the fractionator
    • COI: 1:STN:280:DyaK3MzmsF2nug%3D%3D, PID: 1890236
    • Mayhew TM (1991) Accurate prediction of Purkinje cell number from cerebellar weight can be achieved with the fractionator. J Comp Neurol 308:162–168
    • (1991) J Comp Neurol , vol.308 , pp. 162-168
    • Mayhew, T.M.1
  • 40
    • 84892835466 scopus 로고    scopus 로고
    • Development of myelination and cholinergic innervation in the central auditory system of a prosimian primate (Otolemur garnetti)
    • COI: 1:CAS:528:DC%2BC3sXhsFGgur3M, PID: 23749337
    • Miller DJ, Lackey EP, Hackett TA, Kaas JH (2013) Development of myelination and cholinergic innervation in the central auditory system of a prosimian primate (Otolemur garnetti). J Comp Neurol 521:3804–3816
    • (2013) J Comp Neurol , vol.521 , pp. 3804-3816
    • Miller, D.J.1    Lackey, E.P.2    Hackett, T.A.3    Kaas, J.H.4
  • 41
    • 84901304064 scopus 로고    scopus 로고
    • Three counting methods agree on cell and neuron number in chimpanzee primary visual cortex
    • PID: 24904305
    • Miller DJ, Balaram P, Young N, Kaas JH (2014) Three counting methods agree on cell and neuron number in chimpanzee primary visual cortex. Front Neuroanat 8:36. doi:10.3389/fnana.2014.00036
    • (2014) Front Neuroanat , vol.8 , pp. 36
    • Miller, D.J.1    Balaram, P.2    Young, N.3    Kaas, J.H.4
  • 42
    • 0026697724 scopus 로고
    • NeuN, a neuronal specific nuclear protein in vertebrates
    • COI: 1:CAS:528:DyaK3sXktlGi, PID: 1483388
    • Mullen RJ, Buck CR, Smith AM (1992) NeuN, a neuronal specific nuclear protein in vertebrates. Development 116:201–211
    • (1992) Development , vol.116 , pp. 201-211
    • Mullen, R.J.1    Buck, C.R.2    Smith, A.M.3
  • 43
    • 0023950504 scopus 로고
    • Total number of neurons and glial cells in human brain nuclei estimated by the disector and the fractionator
    • COI: 1:STN:280:DyaL1czgtVOruw%3D%3D, PID: 3043005
    • Pakkenberg B, Gundersen HJ (1988) Total number of neurons and glial cells in human brain nuclei estimated by the disector and the fractionator. J Microsc 150:1–20
    • (1988) J Microsc , vol.150 , pp. 1-20
    • Pakkenberg, B.1    Gundersen, H.J.2
  • 44
    • 0031814240 scopus 로고    scopus 로고
    • Feature article: are neurons lost from the primate cerebral cortex during normal aging?
    • COI: 1:STN:280:DyaK1czhtlWntA%3D%3D, PID: 9651126
    • Peters A, Morrison JH, Rosene DL, Hyman BT (1998) Feature article: are neurons lost from the primate cerebral cortex during normal aging? Cereb Cortex 8:295–300
    • (1998) Cereb Cortex , vol.8 , pp. 295-300
    • Peters, A.1    Morrison, J.H.2    Rosene, D.L.3    Hyman, B.T.4
  • 45
    • 0034255025 scopus 로고    scopus 로고
    • Subnucleus-specific loss of neurons in medial thalamus of schizophrenics
    • COI: 1:CAS:528:DC%2BD3cXls12ksbc%3D, PID: 10908653
    • Popken GJ, Bunney WE Jr, Potkin SG, Jones EG (2000) Subnucleus-specific loss of neurons in medial thalamus of schizophrenics. Proc Natl Acad Sci U S A 97:9276–9280
    • (2000) Proc Natl Acad Sci U S A , vol.97 , pp. 9276-9280
    • Popken, G.J.1    Bunney, W.E.2    Potkin, S.G.3    Jones, E.G.4
  • 46
    • 0029821150 scopus 로고    scopus 로고
    • Preserved neuron number in the hippocampus of aged rats with spatial learning deficits
    • COI: 1:CAS:528:DyaK28XlsFehu7k%3D, PID: 8790433
    • Rapp PR, Gallagher M (1996) Preserved neuron number in the hippocampus of aged rats with spatial learning deficits. Proc Natl Acad Sci U S A 93:9926–9930
    • (1996) Proc Natl Acad Sci U S A , vol.93 , pp. 9926-9930
    • Rapp, P.R.1    Gallagher, M.2
  • 47
    • 0030053883 scopus 로고    scopus 로고
    • Memory impaired aged rats: no loss of principal hippocampal and subicular neurons
    • COI: 1:STN:280:DyaK283ks1Oksg%3D%3D, PID: 8786797
    • Rasmussen T, Schliemann T, Sørensen JC, Zimmer J, West MJ (1996) Memory impaired aged rats: no loss of principal hippocampal and subicular neurons. Neurobiol Aging 17:143–147
    • (1996) Neurobiol Aging , vol.17 , pp. 143-147
    • Rasmussen, T.1    Schliemann, T.2    Sørensen, J.C.3    Zimmer, J.4    West, M.J.5
  • 48
    • 84885057930 scopus 로고    scopus 로고
    • The human cerebral cortex is neither one nor many: neuronal distribution reveals two quantitatively different zones in the grey matter, three in the white matter, and explains local variations in cortical folding
    • PID: 24032005
    • Ribeiro PFM, Ventura-Antunes L, Gabi M, Mota B, Grinberg LT, Farfel JM, Ferretti REL, Leite REP, Jacob Filho W, Herculano-Houzel S (2013) The human cerebral cortex is neither one nor many: neuronal distribution reveals two quantitatively different zones in the grey matter, three in the white matter, and explains local variations in cortical folding. Front Neuroanat 7:28
    • (2013) Front Neuroanat , vol.7 , pp. 28
    • Ribeiro, P.F.M.1    Ventura-Antunes, L.2    Gabi, M.3    Mota, B.4    Grinberg, L.T.5    Farfel, J.M.6    Ferretti, R.E.L.7    Leite, R.E.P.8    Jacob Filho, W.9    Herculano-Houzel, S.10
  • 49
    • 0033789892 scopus 로고    scopus 로고
    • Recommendations for straightforward and rigorous methods of counting neurons based on a computer simulation approach
    • COI: 1:STN:280:DC%2BD3M%2FotFSgsQ%3D%3D, PID: 11074347
    • Schmitz C, Hof PR (2000) Recommendations for straightforward and rigorous methods of counting neurons based on a computer simulation approach. J Chem Neuroanat 20:93–114
    • (2000) J Chem Neuroanat , vol.20 , pp. 93-114
    • Schmitz, C.1    Hof, P.R.2
  • 50
    • 11844300345 scopus 로고    scopus 로고
    • Design-based stereology in neuroscience
    • COI: 1:CAS:528:DC%2BD2MXksVerug%3D%3D, PID: 15652981
    • Schmitz C, Hof PR (2005) Design-based stereology in neuroscience. Neuroscience 130:813–831
    • (2005) Neuroscience , vol.130 , pp. 813-831
    • Schmitz, C.1    Hof, P.R.2
  • 51
    • 0032797237 scopus 로고    scopus 로고
    • Design-based counting techniques: the real problems
    • COI: 1:STN:280:DyaK1Mzjslyksw%3D%3D, PID: 10407447
    • Schmitz C, Korr H, Heinsen H (1999) Design-based counting techniques: the real problems. Trends Neurosci 22:345–346
    • (1999) Trends Neurosci , vol.22 , pp. 345-346
    • Schmitz, C.1    Korr, H.2    Heinsen, H.3
  • 52
    • 34247350053 scopus 로고    scopus 로고
    • Stereologic analysis of the lateral geniculate nucleus of the thalamus in normal and schizophrenic subjects
    • COI: 1:CAS:528:DC%2BD2sXkslektrs%3D, PID: 17383740
    • Selemon LD, Begovic A (2007) Stereologic analysis of the lateral geniculate nucleus of the thalamus in normal and schizophrenic subjects. Psychiatry Res 151:1–10
    • (2007) Psychiatry Res , vol.151 , pp. 1-10
    • Selemon, L.D.1    Begovic, A.2
  • 53
    • 70949083465 scopus 로고    scopus 로고
    • Correlations of neuronal and microvascular densities in murine cortex revealed by direct counting and colocalization of nuclei and vessels
    • COI: 1:CAS:528:DC%2BD1MXhsVOhsLbK, PID: 19923289
    • Tsai PS, Kaufhold JP, Blinder P, Friedman B, Drew PJ, Karten HJ, Lyden PD, Kleinfeld D (2009) Correlations of neuronal and microvascular densities in murine cortex revealed by direct counting and colocalization of nuclei and vessels. J Neurosci 29:14553–14570
    • (2009) J Neurosci , vol.29 , pp. 14553-14570
    • Tsai, P.S.1    Kaufhold, J.P.2    Blinder, P.3    Friedman, B.4    Drew, P.J.5    Karten, H.J.6    Lyden, P.D.7    Kleinfeld, D.8
  • 55
    • 0016654403 scopus 로고
    • Electron microscopical reconstruction of the anterior sensory anatomy of the nematode Caenorhabditis elegans
    • COI: 1:STN:280:DyaE2M%2FpsF2gsA%3D%3D, PID: 1112927
    • Ward S, Thomson N, White JG, Brenner S (1975) Electron microscopical reconstruction of the anterior sensory anatomy of the nematode Caenorhabditis elegans. J Comp Neurol 160:313–337
    • (1975) J Comp Neurol , vol.160 , pp. 313-337
    • Ward, S.1    Thomson, N.2    White, J.G.3    Brenner, S.4
  • 56
    • 0033083082 scopus 로고    scopus 로고
    • Stereological methods for estimating the total number of neurons and synapses: issues of precision and bias
    • COI: 1:CAS:528:DyaK1MXhtVCrtLg%3D, PID: 10092043
    • West MJ (1999) Stereological methods for estimating the total number of neurons and synapses: issues of precision and bias. Trends Neurosci 22:51–61
    • (1999) Trends Neurosci , vol.22 , pp. 51-61
    • West, M.J.1
  • 57
    • 0025327731 scopus 로고
    • Unbiased stereological estimation of the number of neurons in the human hippocampus
    • COI: 1:STN:280:DyaK3c3ptlWqtA%3D%3D, PID: 2358525
    • West MJ, Gundersen HJ (1990) Unbiased stereological estimation of the number of neurons in the human hippocampus. J Comp Neurol 296:1–22
    • (1990) J Comp Neurol , vol.296 , pp. 1-22
    • West, M.J.1    Gundersen, H.J.2
  • 58
    • 0024268109 scopus 로고
    • Three-dimensional counting: an accurate and direct method to estimate numbers of cells in sectioned material
    • COI: 1:STN:280:DyaL1M7gvFKltw%3D%3D, PID: 3216047
    • Williams RW, Rakic P (1988) Three-dimensional counting: an accurate and direct method to estimate numbers of cells in sectioned material. J Comp Neurol 278:344–352
    • (1988) J Comp Neurol , vol.278 , pp. 344-352
    • Williams, R.W.1    Rakic, P.2
  • 59
  • 60
    • 84863974440 scopus 로고    scopus 로고
    • Use of flow cytometry for high-throughput cell population estimates in brain tissue
    • PID: 22798947
    • Young NA, Flaherty DK, Airey DC, Varlan P, Aworunse F, Kaas JH (2012) Use of flow cytometry for high-throughput cell population estimates in brain tissue. Front Neuroanat 6:27. doi:10.3389/fnana.2012.00027
    • (2012) Front Neuroanat , vol.6 , pp. 27
    • Young, N.A.1    Flaherty, D.K.2    Airey, D.C.3    Varlan, P.4    Aworunse, F.5    Kaas, J.H.6
  • 62
    • 84873727728 scopus 로고    scopus 로고
    • Cell and neuron densitites in the primary motor cortex of primates
    • PID: 23450743
    • Young NA, Collins CE, Kaas JH (2013b) Cell and neuron densitites in the primary motor cortex of primates. Front Neural Circuits 7:30
    • (2013) Front Neural Circuits , vol.7 , pp. 30
    • Young, N.A.1    Collins, C.E.2    Kaas, J.H.3
  • 63
    • 0011318985 scopus 로고
    • The determination of deoxyribonucleic acid and of cell number in brain
    • COI: 1:CAS:528:DyaF2MXns1Ghsg%3D%3D, PID: 14200773
    • Zamenhof S, Bursztyn H, Rich K, Zamenhof PJ (1964) The determination of deoxyribonucleic acid and of cell number in brain. J Neurochem 11:505–509
    • (1964) J Neurochem , vol.11 , pp. 505-509
    • Zamenhof, S.1    Bursztyn, H.2    Rich, K.3    Zamenhof, P.J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.