A rapid and reliable method of counting neurons and other cells in brain tissue: A comparison of flow cytometry and manual counting methods

Frontiers in Neuroanatomy

Volumn , Issue FEB, 2010, Pages

  Collins, Christine E ^a   Young, Nicole A ^a   Flaherty, David K ^b   Airey, David C ^a   Kaas, Jon H ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Flow cytometry; Isotropic fractionator; Neubauer chamber; Neuron number

Indexed keywords

ACCURACY; ANALYTICAL EQUIPMENT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BABOON; BRAIN CORTEX; BRAIN TISSUE; CELL COUNT; CONTROLLED STUDY; FLOW CYTOMETER; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPE; FLUORESCENCE MICROSCOPY; HEMISPHERE; INTERMETHOD COMPARISON; NERVE CELL; NEUBAUER CHAMBER COUNT; NONHUMAN; RELIABILITY; REPRODUCIBILITY; SUBCORTEX;

EID: 77951676854     PISSN: 16625129     EISSN: None     Source Type: Journal    
DOI: 10.3389/neuro.05.005.2010     Document Type: Article

References (12)

1. Bland, J. M., and Altman, D. G. (1986). Statistical methods for assessing agreement between two methods of clinical measurement. Lancet, 1, 307-310.
2. Christensen, J. R. Larsen, K. B., Lisanby, S. H., Scalia, J., Arango, V., Dwork, A. J., and Pakkenberg, B. (2007). Neocortical and hippocampal neuron and glial cell numbers in the rhesus monkey. Anat. Rec., 290, 330-340.
3. Dunn, G. (2004). Statistical Evaluation of Measurement Errors: Design and Analysis of Reliability Studies. London, Arnold.
4. Herculano-Houzel, S., Collins, C. E., Wong, P., and Kaas, J. H. (2007). Cellular scaling rules for primate brains. Proc. Natl. Acad. Sci. U.S.A., 104, 3562-3567.
5. Herculano-Houzel, S., Collins, C. E., Wong, P., Kaas, J. H., and Lent, R. (2008). The basic nonuniformity of the cerebral cortex. Proc. Natl. Acad. Sci. U.S.A., 105, 12593-12598.
6. Herculano-Houzel, S., and Lent, R. (2005). Isotropic fractionator: a simple, rapid method for the quantification of total cell and neuron numbers in the brain. Proc. Natl. Acad. Sci. U.S.A., 25, 2518-2521.
7. Lin, L. I.-K. (1989). A concordance correlation coefficient to evaluate reproducibility. Biometrics, 45, 255-268.
8. Lin, L. I.-K. (2000). A note on the concordance correlation coefficient. Biometrics, 56, 324-325.
9. Magari, R. T. (2004). Bias estimation in method comparison studies. J. Biopharm. Stat., 14, 881-892.
10. Rhine, R. J., Norton, G. W., and Wasser, S. K. (2000). Lifetime reproductive success, longevity, and reproductive life history of female yellow baboons (Papio cynocephalus) of Mikumi National Park, Tanzania. Am. J. Primatol., 51, 229-241.
11. Schmitz, C., and Hof, P. R. (2005). Design-based stereology in neuroscience. Neuroscience, 130, 813-831.
12. Surchev, L., Nazwar, T. A., Weisheit, G., and Schilling, K. (2007). Developmental increase of total cell numbers in the murine cerebellum. The Cerebellum, 6, 315-320.