Two distinct events, section compression and loss of particles ("lost caps"), contribute to z-axis distortion and bias in optical disector counting

Microscopy Research and Technique

Volumn 69, Issue 9, 2006, Pages 738-756

  Bakyshnikova, Larisa M ^a   Von Bohlen Und Halbach, Oliver ^b   Kaplan, Suleyman ^c   Von Bartheld, Christopher S ^a  

^a UNIVERSITY OF NEVADA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c ONDOKUZ MAYIS UNIVERSITY SCHOOL OF MEDICINE   (Turkey)

Author keywords

Cell count; Knife angle; Paraffin; Particle count; Sampling; Stereology; Tissue section; Vibratome

Indexed keywords

CUTTING; TISSUE; TISSUE ENGINEERING;

CELL COUNT; KNIFE ANGLES; OPTICAL-; PARTICLE DENSITIES; PARTICLE-COUNTS; RODENT BRAIN; SEQUENCE OF EVENTS; STEREOLOGY; TISSUE SECTIONS; VIBRATOME;

PARAFFINS;

PARAFFIN;

ANIMAL TISSUE; ARTICLE; ARTIFACT; BRAIN TISSUE; CHICKEN; CONTROLLED STUDY; DESICCATION; EMBEDDING; FROZEN SECTION; HUMAN; HUMAN TISSUE; KNIFE ANGLE; MELTING POINT; MICROTOME; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUALITY CONTROL; RAT; STAINING; STEREOLOGY; TISSUE FIXATION; TISSUE SECTION; VIBRATOME;

RODENTIA;

EID: 33748623786     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20345     Document Type: Article

References (52)

1. Abercrombie M. 1946. Estimation of nuclear populations from microtome sections. Anat Rec 94:239-247.
2. Abusaad I, MacKay D, Zhao J, Stanford P, Collier DA, Everall IP. 1999. Stereological estimation of the total number of neurons in the murine hippocampus using the optical disector. J Comp Neurol 408:560-566.
3. Allison RT. 1979. The crystalline nature of histology waxes: The effects of microtomy on the micro-structure of paraffin wax sections. Med Lab Sci 36:359-372.
4. Andersen BB, Gundersen HJ. 1999. Pronounced loss of cell nuclei and anisotropic deformation of thick sections. J Microsc 196:69-73.
5. Andersen BB, Fabricius K, Gundersen HJ, Jelsing J, Stark AK. 2004. No change in neuron numbers in the dentate nucleus of patients with schizophrenia estimated with a new stereological method-The smooth fractionator. J Anat 205:313-321.
6. Bedi KS, Campbell LF, Mayhew TM. 1992. A fractionator study of the effects of undernutrition during early life on rat Purkinje cell numbers (with a caveat on the use of nucleoli as counting units). J Anat 181:199-208.
7. Benes FM, Lange N. 2001. Two-dimensional versus three-dimensional cell counting: A practical perspective. Trends Neurosci 24:11-17.
8. Boonstra H, Oosterhuis JW, Oosterhuis AM, Fleuren GJ. 1983. Cervical tissue shrinkage by formaldehyde fixation, paraffin wax embedding, section cutting and mounting. Virchows Arch A Pathol Anat Histopathol 402:195-201.
9. Calhoun ME, Jucker M, Martin LJ, Thinakaran G, Price DL, Mouton PR. 1996. Comparative evaluation of synaptophysin-based methods for quantification of synapses. J Neurocytol 25:821-828.
10. Clarke PGH. 1993. An unbiased correction factor for cell counts in histological sections. J Neurosci Methods 49:133-140.
11. Coggeshall RE, Lekan HA. 1996. Methods for determining numbers of cells and synapses: A case for more uniform standards of review. J Comp Neurol 364:6-15.
12. Copeland DE. 1941. The surface staining of paraffin sections. Anat Rec 81(Suppl)52-53.
13. Dempster WT. 1942. The mechanics of paraffin sectioning by the microtome. Anat Rec 84:241-267.
14. Dempster WT. 1943. Paraffin compression due to the rotary microtome. Stain Technol 18:13-24.
15. Dorph-Petersen KA, Nyengaard JR, Gundersen HJ. 2001. Tissue shrinkage and unbiased stereological estimation of particle number and size. J Microsc 204:232-246.
16. Dorph-Petersen KA, Rosenberg R, Nyengaard JR. 2004a. Estimation of number and volume of immunohistochemically stained neurons in complex brain regions. In: Evans SM, Janson AM, Nyengaard JR, editors. Quantitative methods in neuroscience. A neuroanatomical approach. Oxford: Oxford University Press, pp. 231-232.
17. Dorph-Petersen KA, Pierri JN, Sun Z, Sampson AR, Lewis DA. 2004b. Stereological analysis of the mediodorsal thalamic nucleus in schizophrenia: Volume, neuron number, and cell types. J Comp Neurol 472:449-462.
18. Evans SM, Nyengaard JR. 2004. Section introduction. In: Evans SM, Janson AM, Nyengaard JR, editors. Quantitative methods in neuroscience. A neuroanatomical approach. Oxford: Oxford University Press. pp. 67-84.
19. Farel PB. 2002. Trust, but verify: The necessity of empirical verification in quantitative neurobiology. Anat Rec 269:157-161.
20. Gardella D, Hatton WJ, Rind HB, Rosen GD, von Bartheld CS. 2003. Differential distortion of the z-axis in tissue sections: Implications for optical disector counting. J Neurosci Methods 124:45-59.
21. Geuna S. 2000. Appreciating the difference between design-based and model-based sampling strategies in quantitative morphology of the nervous system. J Comp Neurol 427:333-339.
22. Geuna S. 2005. The revolution of counting "tops": Two decades of the disector principle in morphological research. Microsc Res Tech 66:270-274.
23. Gordon KC, Bradbury P. 1990. Microtomy and paraffin sections. In: Bancroft JD, Stevens A, editors. Theory and practice of histological techniques, 3rd ed. Edinburgh: Churchill Livingstone. pp. 61-80.
24. Guillery RW. 2002. On counting and counting errors. J Comp Neurol 447:1-7.
25. Guillery RW, August BK. 2002. Doubt and certainty in counting. Prog Brain Res 135:25-42.
26. Guillery RW, Herrup K. 1997. Quantification without pontification: Choosing a method for counting objects in sectioned tissues. J Comp Neurol 386:2-7.
27. Gundersen HJG. 1986. Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J Microsc 143:3-45.
28. Gundersen HJG, Bagger P, Bendtsen TF, Evans SM, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ. 1988. The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. Acta Pathol Microbiol Immunol Scand 96:857-881.
29. Hatton WJ, von Bartheld CS. 1999. Analysis of cell death in the trochlear nucleus of chick embryos: Calibration of the optical disector counting technique reveals systematic bias. J Comp Neurol 409:169-186.
30. Haug H, Kuhl S, Mecke E, Sass NL, Wasner K. 1984. The significance of morphometric procedures in the investigation of age changes in cytoarchitectonic structures of human brain. J Hirnforsch 25:353-374.
31. Hedreen JC. 1998. Lost caps in histological counting methods. Anat Rec 25:366-372.
32. Helander KG. 1983. Thickness variations within individual paraffin and glycol methacrylate sections. J Microsc 132:223-227.
33. Howard CV, Reed MG. 1998. Unbiased stereology. Three-dimensional measurement in microscopy. New York: Springer. 246 p.
34. Konigsmark BW 1970. Methods for the counting of neurons. In: Nauta WJH, Ebbesson SOE, editors. Contemporary research methods in neuroanatomy. New York: Springer, pp. 315-340.
35. Mayhew TM, Gundersen HJ. 1996. 'If you assume, you can make an ass out of u and me': A decade of the disector for stereological counting of particles in 3D space. J Anat 188:1-15.
36. Mouton PR. 2002. Principles and practices of unbiased stereology. An introduction for bioscientists. Baltimore: Johns Hopkins University Press. 214 p.
37. Myers M, Britt KL, Wreford NG, Ebling FJ, Kerr JB. 2004. Methods for quantifying follicular numbers within the mouse ovary. Reproduction 127:569-580.
38. Pelvig DP, Pakkenberg H, Regeur L, Oster S, Pakkenberg B. 2003. Neocortical glial cell numbers in Alzheimer's disease. A stereological study. Dement Geriatr Cogn Disord 16:212-219.
39. Schmitz C, Hof PR. 2005. Design-based stereology in neuroscience. Neuroscience 130:813-831.
40. Schmitz C, Korr H, Heinsen H. 1999. Design-based counting techniques: The real problems. Trends Neurosci 22:345-346.
41. Schmitz C, Dafotakis M, Heinsen H, Mugrauer K, Niesel A, Popken GJ, Stephan M, Van de Berg WD, von Horsten S, Korr H. 2000. Use of cryostat sections from snap-frozen nervous tissue for combining stereological estimates with histological, cellular, or molecular analyses on adjacent sections. J Chem Neuroanat 20:21-29.
42. Tandrup T. 2004. Unbiased estimates of number and size of rat dorsal root ganglion cells in studies of structure and cell survival. J Neurocytol 33:173-192.
43. Tune AT, Turgut M, Aslan H, Sahin B, Yurtseven ME, Kaplan S. 2006. Neonatal pinealectomy induces Purkinje cell loss in the cerebellum of the chick: A stereological study. Brain Res 1067:95-102.
44. Vacca LL. 1985. Laboratory manual of histochemistry. New York: Raven. 578 p.
45. -3mm Stärke für elektronen mikroskopische Zwecke. Z Wiss Mikrosk 56:8-23.
46. von Bartheld CS. 1999. Systematic bias in an 'unbiased' neuronal counting technique. Anat Rec B New Anat 257:119-120.
47. von Bartheld CS. 2001. Comparison of 2D- and 3D-counting: The need for calibration and common sense. Trends Neurosci 24:504-506.
48. von Bartheld CS. 2002. Counting particles in tissue sections: Choices of methods and importance of calibration to minimize biases. Histol Histopathol 17:639-648.
49. von Bohlen und Halbach O, Minichiello L, Unsicker K. 2005. Haploinsufficiency for trkB and trkC receptors induces cell loss and accumulation of α-synuclein in the substantia nigra. FASEB J 19:1740-1742.
50. West MJ. 1999. Stereological methods for estimating the total number of neurons and synapses: Issues of precision and bias. Trends Neurosci 22:51-61.
51. Williams RW, Rakic P. 1988. Three-dimensional counting: Accurate and direct method to estimate numbers of cells in sectioned material. J Comp Neurol 278:344-352.
52. Williams RW, von Bartheld CS, Rosen GD. 2003. Counting cells in sectioned material: A suite of techniques, tools and tips. New York: John Wiley & Sons, Inc. Current Protocols in Neuroscience Vol. 1, Supplement 24 1.11.1-1.11.29.