Counting particles in tissue sections: Choices of methods and importance of calibration to minimize biases

Histology and Histopathology

Volumn 17, Issue 2, 2002, Pages 639-648

  Von Bartheld, Christopher S ^a  

^a UNIVERSITY OF NEVADA SCHOOL OF MEDICINE   (United States)

Author keywords

Bias; Calibration; Optical disector; Sampling; Stereology

Indexed keywords

ACCURACY; CALIBRATION; CELL COUNT; CELL SHAPE; CELL SIZE; CELLULAR DISTRIBUTION; CLINICAL OBSERVATION; CLINICAL PROTOCOL; COMPARATIVE STUDY; CONTROLLED STUDY; HUMAN; IMAGE CYTOMETRY; LABORATORY TEST; OBSERVER VARIATION; OPTICAL INSTRUMENTATION; QUANTITATIVE ANALYSIS; REVIEW; STEREOLOGY; TECHNIQUE; THREE DIMENSIONAL IMAGING; TISSUE SECTION;

ANIMALS; CALIBRATION; CELL COUNT; HUMANS; MICROTOMY;

EID: 0036108605     PISSN: 02133911     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (48)

1. Estimation of nuclear populations from microtome sections
2. Two-dimensional versus three-dimensional cell counting: A practical perpective
3. Quantitative morphology of the nervous systems: Expanding horizons
4. The impact of recent stereological advances on quantitative studies of the nervous system
5. Neuron loss in APP transgenic mice
6. How inaccurate is the Abercrombie correction factor for cell counts?
7. An unbiased correction factor for cell counts in histological sections
8. Neuron death in vertebrate development: In vitro methods
9. A consideration of neural counting methods
10. Methods for determining numbers of cells and synapses: A case for more uniform standards of review
11. Calibration of methods for determining numbers of dorsal root ganglion cells
12. Towards a more objective biology
13. Hippocampal neurodegeneration in aging
14. Ueber die Zahlen der Nervenfasern und Ganglienzellen in den Spinalganglien des Kaninchens
15. Appreciating the difference between design-based and model-based sampling strategies in quantitative morphology of the nervous system
16. Quantification without pontification: Choosing a method for counting objects in sectioned tissues
17. The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathology research and diagnosis
18. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis
19. Simplified nerve cell counting in the rat brainstem with the physical disector using a drawing-mciroscope
20. Practical considerations for the use of the optical disector in estimating neuronal number
21. Analysis of cell death in the trochlear nucleus of chick embryos: Calibration of the optical disector counting technique reveals systematic bias
22. Lost caps in histological counting methods
23. What was wrong with the Abercrombie and empirical cell counting methods? A review
24. Centenary of Gaule and Lewin, pioneers in cell counting methodology
25. Celloidin mounting (embedding without infiltration) - A new, simple and reliable method for producing serial sections of high thickness through complete human brains and its application to stereological and immunohistochemical investigations
26. Hippocampal volume and neuronal number in Ts65Dn mice: A murine model of Down syndrome
27. Methods for the counting of neurons
28. A simplified application of systematic field sampling and low-cost video recording set-up for viewing disector pairs - Exemplified in the rat cochlear nucleus
29. What counts in brain aging? Design-based stereological analysis of cell number
30. A review of recent advances in stereology for quantifying neural structure
31. 'If you assume, you can make an ass out of u and me': A decade of the disector for stereological counting of particles in 3D space
32. Life and death of neurons in the aging brain
33. Reliability and validity of the physical disector method for estimating neuron number
34. Sensory neuron number in neonatal and adult rats estimated by means of stereologic and profile-based methods
35. Verification of the disector method for counting neurons, with comments on the empirical method
36. Any way you cut it: A new journal policy for the use of unbiased counting methods
37. Unbiased stereology: Three-dimensional measurement in microscopy
38. The number of neurons in dorsal root ganglia L4-L6 of the rat
39. Recommendations for straightforward and rigorous methods of counting neurons based on a computer simulation approach
40. Design-based counting techniques: The real problems
41. Systematic bias in an 'unbiased' neuronal counting technique
42. Comparison of 2D- and 3D-counting: The need for calibration and common sense
43. New stereological methods for counting neurons
44. Stereological methods for estimating the total number of neurons and synapses: Issues of precision and bias
45. Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator
46. Is hippocampal cell death a myth?
47. Three-dimensional counting: Accurate and direct method to estimate numbers of cells in sectioned material