Genome-wide fitness and genetic interactions determined by Tn-seq, a high-throughput massively parallel sequencing method for microorganisms

Current Protocols in Microbiology

Volumn 2015, Issue , 2015, Pages 1E.3.1-1E.3.24

  van Opijnen, Tim ^a   Lazinski, David W ^b   Camilli, Andrew ^b  

^a BOSTON COLLEGE   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Genetic interactions; Genome wide fitness; Massively parallel sequencing; Streptococcus pneumoniae; Tn seq; Transposon mutagenesis; Transposon sequencing; Vibrio cholerae

Indexed keywords

DNA POLYMERASE; GENOMIC DNA; HIMAR1 MARINER MARC9 TRANSPOSASE; MALTOSE BINDING PROTEIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; T4 DNA POLYMERASE; TRANSPOSASE; UNCLASSIFIED DRUG; TRANSPOSON;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL GENE; BACTERIUM CONJUGATION; BACTERIUM CULTURE; BACTERIUM TRANSFORMATION; CELL LYSATE; CLINICAL PROTOCOL; DNA ISOLATION; DNA SEQUENCE; DNA TRANSPOSITION; GENE INSERTION; GENE INTERACTION; GENE LIBRARY; GENETIC PARAMETERS; GENOME WIDE FITNESS; HOMOLOGOUS RECOMBINATION; IN VITRO STUDY; INVERTED REPEAT; MAGELLAN6 GENE; MICROORGANISM; MMEI GENE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; STREPTOCOCCUS PNEUMONIAE; TRANSPOSON; TRANSPOSON SEQUENCING; VIBRIO CHOLERAE; GENE EXPRESSION REGULATION; HIGH THROUGHPUT SEQUENCING; MICROBIAL GENETICS; MOLECULAR BIOLOGY; MOLECULAR GENETICS; PROCEDURES;

DNA TRANSPOSABLE ELEMENTS; GENETICS, MICROBIAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE ANNOTATION; MUTAGENESIS, INSERTIONAL;

EID: 84938329670     PISSN: 19348525     EISSN: 19348533     Source Type: Journal    
DOI: 10.1002/9780471729259.mc01e03s36     Document Type: Article

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