Homopolymer tail-mediated ligation PCR: A streamlined and highly efficient method for DNA cloning and library construction

BioTechniques

Volumn 54, Issue 1, 2013, Pages 25-34

  Lazinski, David W ^a   Camilli, Andrew ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Annealing assisted ligation; DNA capture; Massively parallel sequencing; Molecular cloning

Indexed keywords

DNA NUCLEOTIDYLEXOTRANSFERASE; DNA POLYMERASE; OLIGONUCLEOTIDE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PRIMER DNA;

ARTICLE; DNA LIBRARY; DNA TEMPLATE; DNA TRANSPOSITION; EXTRACTION; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE SEQUENCE; HOMOPOLYMER TAIL MEDIATED LIGATION POLYMERASE CHAIN REACTION; HYBRID; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; SINGLE NUCLEOTIDE POLYMORPHISM; STREPTOCOCCUS PNEUMONIAE; TEMPERATURE; VIBRIO CHOLERAE;

CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; GENE LIBRARY; POLYMERASE CHAIN REACTION; POLYNUCLEOTIDES; STREPTOCOCCUS PNEUMONIAE; VIBRIO CHOLERAE;

EID: 84874370010     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113981     Document Type: Article

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