Microvolume quantitation of nucleic acids

Current Protocols in Molecular Biology

Volumn , Issue SUPPL.93, 2011, Pages

  Desjardins, Philippe R ^a   Conklin, Deborah S ^a  

^a Thermo Scientific NanoDrop Products   (United States)

Author keywords

Absorbance; DNA; Fluorescence; Microvolume; Quantitation; RNA; Spectroscopy

Indexed keywords

ANALYTIC METHOD; ARTICLE; CONCENTRATION (PARAMETERS); EQUIPMENT DESIGN; INTERMETHOD COMPARISON; MICROANALYSIS; NUCLEIC ACID ANALYSIS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SPECTROFLUOROMETRY; SPECTROPHOTOMETRY; INSTRUMENTATION; METHODOLOGY; NANOTECHNOLOGY; ULTRAVIOLET SPECTROPHOTOMETRY; CONTROLLED STUDY; FLUOROSPECTROMETER; MICROCELL CUVETTE; SPECTROMETER; SPECTROPHOTOMETER;

NUCLEIC ACID; DOUBLE STRANDED DNA; OLIGONUCLEOTIDE; RNA; SINGLE STRANDED DNA;

NANOTECHNOLOGY; NUCLEIC ACIDS; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 79953281582     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mba03js93     Document Type: Article

References (4)

1. Glasel, J.A. 1995. Validity of nucleic acid purities monitoredbyA260/A280 absorbance ratios. Biotechniques 18:62-63.
2. Ingle, J.D. Jr. and Crouch, S.R. 1988. Spectrochemical Analysis. XV +. Prentice Hall, Englewood Cliffs, N.J.
3. Voolstra, C., Jungnickel, A., Borrmann, L., Kirchner, R., and Huber, A. 2006. Spectrophotometric Quantification of Nucleic Acids: LabelGuard Enables Photometric Quantification of Submicroliter Samples Using a Standard Photometer. Implen Applications Note, Munich, Germany.
4. Wilfinger, W.W., Mackey, K., and Chomczynski, P. 1997. Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity: BioTechniques 22:474-481.