Modulation of talA gene in pentose phosphate pathway for overproduction of poly-β-hydroxybutyrate in transformant Escherichia coli harboring phbCAB operon

Journal of Bioscience and Bioengineering

Volumn 102, Issue 3, 2006, Pages 237-240

  Song, Byoung Geun ^a   Kim, Tae Kwon ^a   Jung, Young Mi ^a   Lee, Yong Hyun ^a  

^a Kyungpook National University   (South Korea)

Author keywords

metabolic engineering; pentose phosphate pathway; phbCAB operon; poly hydroxybutyrate (PHB) biosynthesis; talA gene; transaldolase; transformant Escherichia coli

Indexed keywords

METABOLIC ENGINEERING; PENTOSE PHOSPHATE PATHWAY; POLY-HYDROXYBUTYRATE (PHB) BIOSYNTHESIS; TRANSALDOLASE;

BIOSYNTHESIS; ESCHERICHIA COLI; METABOLISM; MODULATION; PHOSPHATES;

GENETIC ENGINEERING;

ACETYL COENZYME A; HEXOSE PHOSPHATE; PENTOSE PHOSPHATE; POLY(3 HYDROXYBUTYRIC ACID); REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRANSALDOLASE;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; CELL GROWTH; CONTROLLED STUDY; ENZYME METABOLISM; ESCHERICHIA COLI; GENE; GENE AMPLIFICATION; GENE EXPRESSION; HEXOSE MONOPHOSPHATE SHUNT; NONHUMAN; OPERON; PENTOSE PHOSPHATE CYCLE; POLYMER PRODUCTION; TALA GENE;

BACTERIAL PROTEINS; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HYDROXYBUTYRATES; OPERON; PENTOSE PHOSPHATE PATHWAY; POLYESTERS; TRANSALDOLASE;

ESCHERICHIA COLI;

EID: 33749835786     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.102.237     Document Type: Article

References (14)

1. Sudesh K., Abe H., and Doi Y. Synthesis, structure and properties of polyhydroxyalknaotes: biological polyesters. Prog. Polym. Sci. 25 (2000) 1503-1555
2. Rehm B.H., Antonio R.V., Spiekermann P., Amara A.A., and Steinbüchel A. Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model. Biochim. Biophys. Acta 1594 (2002) 178-190
3. Shi H., Shiraishi M., and Shimizu K. Metabolic flux analysis for biosynthesis of poly(β-hydroxybutyric acid) in Alcaligenes eutrophus from various carbon sources. J. Ferment. Bioeng. 84 (1997) 579-587
4. Ikeda M., and Katsumata R. Hyperproduction of tryptophan by Corynebacterium glutamicum with the modified pentose phosphate pathway. Appl. Environ. Microbiol. 65 (1999) 2497-2502
5. Choi J.C., Shin H.D., and Lee Y.H. Modulation of 3-hydroxyvalerate molar fraction in poly(3-hydroxybutyrate-3-hydroxyvalerate) using Ralstonia eutropha transformant co-amplifying phbC and NADPH generating-related zwf genes. Enzyme Microb. Technol. 32 (2003) 178-185
6. Lim S.J., Jung Y.M., Shin H.D., and Lee Y.H. Amplification of the NADPH-related genes zwf and gnd for oddball biosynthesis of PHB in an E. coli transformant harboring a cloned phbCAB operon. J. Biosci. Bioeng. 93 (2002) 543-549
7. Kabir M.M., and Shimizu K. Gene expression patterns for metabolic pathway in pgi knockout Escherichia coli with and without phb operon based on RT-PCR. J. Biotechnol. 105 (2003) 11-31
8. Stephanopoulos G.N., Aristidou A.A., and Nielsen J. Review of cellular metabolism. In: Stephanopoulos G.N., Aristidou A.A., and Nielsen J. (Eds). Metabolic engineering (1998), Academic Press, San Diego 21-79
9. Lee J.N., Shin H.D., and Lee Y.H. Metabolic engineering of pentose phosphate pathway in Ralstonia eutropha for enhanced biosynthesis of poly-β-hydroxybutyrate. Biotechnol. Prog. 19 (2003) 1444-1449
10. Jung Y.M., Lee J.N., Shin H.D., and Lee Y.H. Role of tktA gene in pentose phosphate pathway on odd-ball biosynthesis of poly-β-hydroxybutyrate in transformant Escherichia coli harboring a cloned phbCAB operon. J. Biosci. Bioeng. 98 (2004) 224-227
11. Park J.S., Huh T.L., and Lee Y.H. Characteristics of cell growth and poly-β-hydroxybutyrate biosynthesis of Alcaligenes eutrophus transformants harboring cloned phbCAB genes. Enzyme Microb. Technol. 21 (1997) 85-90
12. Sprenger G.A., Schörken U., Sprenger G., and Sahm H. Transaldolase B of Escherichia coli K-12: cloning of its gene, talB, and characterization of the enzyme from recombinant strains. J. Bacteriol. 177 (1995) 5930-5936
13. Soderberg T., and Alver R.C. Transaldolase of Methanocaldococcus jannaschii. Archaea 1 (2004) 255-262
14. Jung Y.M., and Lee Y.H. Investigation of regulatory mechanism of flux of acetyl-CoA in Alcaligenes eutrophus using PHB negative mutant and transformants harboring cloned phbCAB genes. J. Microbiol. Biotechnol. 7 (1997) 215-222