Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate

Metabolic Engineering

Volumn 8, Issue 3, 2006, Pages 281-290

  De Anda, Ramón ^a   Lara, Alvaro R ^a   Hernández, Vanessa ^a   Hernández Montalvo, Verónica ^a   Gosset, Guillermo ^a   Bolívar, Francisco ^a   Ramírez, Octavio T ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

Aerobic acetate reduction; Fed batch; Glucose transport systems engineering; Recombinant E. coli

Indexed keywords

BATCH CELL CULTURE; ESCHERICHIA COLI; FLUORESCENCE; GLUCOSE; GROWTH KINETICS; PROTEINS;

AEROBIC ACETATE REDUCTION; FED-BATCH; GLUCOSE TRANSPORT SYSTEMS ENGINEERING; RECOMBINANT E. COLI;

ENZYME KINETICS;

ACETIC ACID; GALACTOSE PERMEASE; GLUCOKINASE; GREEN FLUORESCENT PROTEIN; PERMEASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CARBON SOURCE; CELL INCLUSION; ENZYME REPLACEMENT; ESCHERICHIA COLI; GENE; GLUCOSE INTAKE; GLUCOSE TRANSPORT; GROWTH RATE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION;

ACETATES; BIOLOGICAL TRANSPORT, ACTIVE; CELL PROLIFERATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENHANCEMENT; GLUCOSE; MONOSACCHARIDE TRANSPORT PROTEINS; PHOSPHOTRANSFERASES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33646082049     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2006.01.002     Document Type: Article

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