NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells

Biotechnology Journal

Volumn 10, Issue 7, 2015, Pages 1019-1028

  Brown, Adam J ^a   Sweeney, Bernie ^b   Mainwaring, David O ^b   James, David C ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b Slough   (United Kingdom)

Author keywords

Biopharmaceutical production; Chinese hamster ovary cells; CMV promoter; Transcriptional regulation; Transient gene expression

Indexed keywords

CELLS; CYTOLOGY; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENES; TRANSCRIPTION FACTORS;

CHINESE HAMSTER OVARY CELLS; CMV PROMOTER; GENE TRANSCRIPTIONS; OPTIMIZATION AND CONTROL; REGULATORY ELEMENTS; REGULATORY FUNCTIONS; TRANSCRIPTIONAL REGULATION; TRANSIENT GENE EXPRESSIONS;

TRANSCRIPTION;

ALKALINE PHOSPHATASE; CRE RECOMBINASE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; SECRETED ALKALINE PHOSPHATASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR YY1; UNCLASSIFIED DRUG; INTEGRASE;

ANIMAL CELL; ARTICLE; BINDING SITE; CHO CELL LINE; COMPUTER MODEL; CONTROLLED STUDY; CYTOMEGALOVIRUS; FEMALE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; TRANSACTIVATION; TRANSIENT EXPRESSION; ANIMAL; BIOSYNTHESIS; CRICETULUS; DRUG DEVELOPMENT; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETICS; HAMSTER;

CRICETULUS GRISEUS; CYTOMEGALOVIRUS;

ALKALINE PHOSPHATASE; ANIMALS; BINDING SITES; CHO CELLS; CRICETINAE; CRICETULUS; CYTOMEGALOVIRUS; DRUG DISCOVERY; GENE EXPRESSION; INTEGRASES; NF-KAPPA B; PROMOTER REGIONS, GENETIC; TRANSCRIPTION, GENETIC; YY1 TRANSCRIPTION FACTOR;

EID: 84935700781     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400744     Document Type: Article

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