Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene with Bcl-xL

Biotechnology Journal

Volumn 9, Issue 9, 2014, Pages 1164-1174

  Zustiak, Matthew P ^a,b   Jose, Lisa ^b   Xie, Yueqing ^b   Zhu, Jianwei ^b   Betenbaugh, Michael J ^a  

^a Johns Hopkins University   (United States)

^b SAIC FREDERICK INC   (United States)

Author keywords

Anti apoptosis; CHO cells; Polyethyleneimine (PEI); Transient gene expression

Indexed keywords

CELL CULTURE; CELL DEATH; GENE EXPRESSION; PRODUCTIVITY; RECOMBINANT PROTEINS; INVENTORY CONTROL; MAMMALS; MOLECULAR BIOLOGY;

ANTI-APOPTOSIS; ANTI-APOPTOTIC PROTEIN; CHO CELL; CONSTITUTIVE EXPRESSIONS; POLYETHYLENEIMINE; RECOMBINANT PROTEIN PRODUCTIONS; SPECIFIC PRODUCTIVITY; TRANSIENT GENE EXPRESSIONS;

MOLECULAR BIOLOGY; GENE EXPRESSION;

POLYETHYLENEIMINE; PROTEIN BCL 2; PROTEIN BCL X; RECOMBINANT PROTEIN; PROTEIN BCL 2 DELTA; UNCLASSIFIED DRUG;

ANIMAL; APOPTOSIS; CELL LINE; CHO CELL LINE; CRICETULUS; GENE EXPRESSION; GENETIC TRANSFECTION; GENETICS; HEK293 CELL LINE; HUMAN; METABOLISM; PROCEDURES; ANIMAL CELL; ARTICLE; BCL XL GENE; CONTROLLED STUDY; FEMALE; GENE; GENE VECTOR; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; TRANSIENT EXPRESSION;

ANIMALS; APOPTOSIS; BCL-X PROTEIN; CELL LINE; CHO CELLS; CRICETULUS; GENE EXPRESSION; HEK293 CELLS; HUMANS; POLYETHYLENEIMINE; PROTO-ONCOGENE PROTEINS C-BCL-2; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 84906809304     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300468     Document Type: Article

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