Synthetic design of strong promoters

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 6, 2010, Pages 2538-2543

  Schlabach, Michael R ^a   Hu, Jimmy K ^a   Li, Mamie ^a   Elledge, Stephen J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

GFP reporter; RNA interference; Synthetic biology; Transcription

Indexed keywords

DNA FRAGMENT; GREEN FLUORESCENT PROTEIN; REPETITIVE DNA;

ANIMAL CELL; ARTICLE; CELL LINE; CMV ENHANCER ELEMENT; CONTROLLED STUDY; DNA SEQUENCE; DNA SYNTHESIS; ENHANCER REGION; FLUORESCENCE ACTIVATED CELL SORTING; HELA CELL; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN MOTIF; RETROVIRUS; TANDEM REPEAT; TRANSCRIPTION INITIATION;

ANIMALS; BINDING SITES; CELL LINE; CYTOMEGALOVIRUS; ENHANCER ELEMENTS, GENETIC; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; MICE; MICROARRAY ANALYSIS; OLIGONUCLEOTIDES; PROMOTER REGIONS, GENETIC; REGULATORY SEQUENCES, NUCLEIC ACID; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EID: 77249137749     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0914803107     Document Type: Article

References (21)

1. Baup D, et al. (2009) Variegation and silencing in a lentiviral-based murine transgenic model. Transgenic Res Aug 23. [Epub ahead of print].
2. Kadonaga JT (2004) Regulation of RNA polymerase II transcription by sequence-specific DNA binding factors. Cell 116:247-257.
3. Juven-Gershon T, Cheng S, Kadonaga JT (2006) Rational design of a super core promoter that enhances gene expression. Nat Methods 3:917-922.
4. Foecking MK, Hofstetter H (1986) Powerful and versatile enhancer-promoter unit for mammalian expression vectors. Gene 45:101-105.
5. Benoist C, Chambon P (1981) In vivo sequence requirements of the SV40 early promotor region. Nature 290:304-310.
6. Sadowski I, Ma J, Triezenberg S, Ptashne M (1988) GAL4-VP16 is an unusually potent transcriptional activator. Nature 335:563-564.
7. Ellis T, Wang X, Collins JJ (2009) Diversity-based, model-guided construction of synthetic gene networks with predicted functions. Nat Biotechnol 27:465-471.
8. Gertz J, Siggia ED, Cohen BA (2009) Analysis of combinatorial cis-regulation in synthetic and genomic promoters. Nature 457:215-218.
9. Gossen M, Bujard H (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 89:5547-5551.
10. Korinek V, et al. (1997) Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma. Science 275:1784-1787.
11. Cleary MA, et al. (2004) Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis. Nat Methods 1:241-248.
12. Silva JM, et al. (2005) Second-generation shRNA libraries covering the mouse and human genomes. Nat Genet 37:1281-1288.
13. Mukherjee S, et al. (2004) Rapid analysis of the DNA-binding specificities of transcription factors with DNA microarrays. Nat Genet 36:1331-1339.
14. Fink JS, et al. (1988) The CGTCA sequence motif is essential for biological activity of the vasoactive intestinal peptide gene cAMP-regulated enhancer. Proc Natl Acad Sci USA 85:6662-6666.
15. Craig JC, et al. (2001) Consensus and variant cAMP-regulated enhancers have distinct CREB-binding properties. J Biol Chem 276:11719-11728.
16. Chung S, et al. (2002) Analysis of different promoter systems for efficient transgene expression in mouse embryonic stem cell lines. Stem Cells 20:139-145.
17. Ramezani A, Hawley TS, Hawley RG (2000) Lentiviral vectors for enhanced gene expression in human hematopoietic cells. Mol Ther 2:458-469.
18. Kandavelou K, et al. (2009) Targeted manipulation of mammalian genomes using designed zinc finger nucleases. Biochem Biophys Res Commun 388:56-61.
19. Berger MF, Bulyk ML (2009) Universal protein-binding microarrays for the comprehensive characterization of the DNA-binding specificities of transcription factors. Nat Protoc 4:393-411.
20. Mandell JG, Barbas CF, III (2006) Zinc finger tools: custom DNA-binding domains for transcription factors and nucleases. Nucleic Acids Res 34:W516-W523.
21. Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ (2008) Global protein stability profiling in mammalian cells. Science 322:918-923.