MEGA (Multiple Essential Genes Assembling) Deletion and Replacement Method for Genome Reduction in Escherichia coli

ACS Synthetic Biology

Volumn 4, Issue 6, 2015, Pages 700-706

  Xue, Xiaoli ^a   Wang, Tao ^a   Jiang, Peng ^a   Shao, Yangyang ^a   Zhou, Min ^a   Zhong, Li ^a   Wu, Ronghai ^a   Zhou, Jianting ^a   Xia, Haiyang ^a   Zhao, Guoping ^a   Qin, Zhongjun ^a  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

Author keywords

essential gene; gene assembly; genome engineering; genome reduction

Indexed keywords

DNA FRAGMENT; ENDONUCLEASE; GENOMIC DNA;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENOME; CONTROLLED STUDY; DELETION MUTANT; DNA STRAND BREAKAGE; ELECTROTRANSFORMATION; ESCHERICHIA COLI; ESSENTIAL GENE; GENE DELETION; GENETIC ANALYSIS; HOMOLOGOUS RECOMBINATION; MULTIPLE ESSENTIAL GENE ASSEMBLING; NONHUMAN; OPTICAL DENSITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SACCHAROMYCES CEREVISIAE; SHUTTLE VECTOR; GENE VECTOR; GENETIC ENGINEERING; GENETICS; PROCEDURES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ESCHERICHIA COLI; GENE DELETION; GENES, ESSENTIAL; GENETIC ENGINEERING; GENETIC VECTORS; GENOME, BACTERIAL; SACCHAROMYCES CEREVISIAE;

EID: 84934912204     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb500324p     Document Type: Article

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