Pulsed-field gel electrophoresis of bacterial chromosomes

Methods in Molecular Biology

Volumn 1054, Issue , 2013, Pages 187-194

  Mawer, Julia S P ^a   Leach, David R F ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Agarose gel electrophoresis; Agarose plugs; Contour clamped homogeneous electric field (CHEF); Escherichia coli; Pulse time; Pulsed field gel electrophoresis (PFGE)

Indexed keywords

PROTEINASE K;

APURINIC SITE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENOME; BACTERIUM COLONY; CHROMOSOME BAND; DNA REPLICATION; DNA STRAND BREAKAGE; ESCHERICHIA COLI; KARYOTYPING; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; CHEMISTRY; CHROMOSOME; GENETICS; MICROBIAL GENOME; PROCEDURES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

CHROMOSOMES; CHROMOSOMES, BACTERIAL; DNA REPLICATION; ELECTROPHORESIS, GEL, PULSED-FIELD; ESCHERICHIA COLI; GENOME, MICROBIAL;

EID: 84922789496     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-565-1_12     Document Type: Article

References (4)

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2. Maule J (1998) Pulsed-field gel electrophoresis. Mol Biotechnol 9:107-126
3. Chu G, Vollrath D, Davis RW (1986) Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234: 1582-1585
4. Gunderson K, Chu G (1991) Pulsed-field electrophoresis of megabase-sized DNA. Mol Cell Biol 11:3348-3354