Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel

Biotechnology and Bioengineering

Volumn 112, Issue 8, 2015, Pages 1683-1695

  Xu, Yufan ^a   Wang, Xiaohong ^a,b  

^a TSINGHUA UNIVERSITY   (China)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

3D printing; Adipose derived stem cells (ADSCs); Hepatocytes; Microfluidics; Parallel alginate gelatin fibrin channels

Indexed keywords

3D PRINTERS; ALGINATE; BIOCOMPATIBILITY; BIOLOGICAL MATERIALS; CELLS; CYTOLOGY; FLUIDIC DEVICES; MOLECULAR BIOLOGY; STEM CELLS;

3-D PRINTING; ADIPOSE DERIVED STEM CELLS; HEPATOCYTES; MICROFLUIDIC TECHNOLOGIES; PARALLEL ALGINATE/GELATIN/FIBRIN CHANNELS; REGENERATIVE MEDICINE; THERAPEUTIC POTENTIALS; THREEDIMENSIONAL (3-D);

MICROFLUIDICS;

ALGINIC ACID; BIOMATERIAL; BLOOD CLOTTING FACTOR 8; FIBRIN; GELATIN; GLUCURONIC ACID; HEXURONIC ACID;

ADIPOSE DERIVED STEM CELL; ANIMAL CELL; ARTICLE; CELL ADHESION; CELL FUNCTION; CELL MANIPULATION; CELL MIGRATION; CELL MOTILITY; CELL STRUCTURE; CELL SUSPENSION; COCULTURE; COMPUTER SIMULATION; ENVIRONMENTAL TEMPERATURE; HYDROGEL; IMMUNOHISTOCHEMISTRY; INTRACELLULAR FLUID; LIVER CELL; MICROFLUIDICS; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SECRETION; RAT; SCANNING ELECTRON MICROSCOPY; THREE DIMENSIONAL PRINTING; CELL DIFFERENTIATION; ENDOTHELIUM CELL; PHYSIOLOGY; PROCEDURES; STEM CELL; TISSUE ENGINEERING; TISSUE SCAFFOLD;

ALGINATES; CELL DIFFERENTIATION; COCULTURE TECHNIQUES; ENDOTHELIAL CELLS; FIBRIN; GELATIN; GLUCURONIC ACID; HEPATOCYTES; HEXURONIC ACIDS; MICROFLUIDICS; STEM CELLS; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 84932198186     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25579     Document Type: Article

References (104)

1. Badylak SF, Weiss DJ, Caplan A, Macchiarini P. 2012. Engineered whole organs and complex tissues. Lancet 379(9819):943-952.
2. Bellan LM, Singh SP, Henderson PW, Porri TJ, Craighead HG, Spector JA. 2009. Fabrication of an artificial 3-dimensional vascular network using sacrificial sugar structures. Soft Matter 5(7):1354-1357.
3. Bhatia SN, Ingber DE. 2014. Microfluidic organs-on-chips. Nat Biotechnol 32(8):760-772.
4. Capel AJ, Edmondson S, Christie SDR, Goodridge RD, Bibb RJ, Thurstans M. 2013. Design and additive manufacture for flow chemistry. Lab Chip 13(23):4583-4590.
5. Choi NW, Cabodi M, Held B, Gleghorn JP, Bonassar LJ, Stroock AD. 2007. Microfluidic scaffolds for tissue engineering. Nat Mater 6(11):908-915.
6. Cui T, Yan Y, Zhang R, Liu L, Xu W, Wang X. 2009. Rapid prototyping of a double layer polyurethane-collagen conduit for peripheral nerve regeneration. Tissue Eng Part C Methods 15(1):1-9.
7. Cushing MC, Anseth KS. 2007. Hydrogel cell cultures. Science 316(5828):1133-1134.
8. Cushing MC, Mariner PD, Liao JT, Sims EA, Anseth KS. 2008. Fibroblast growth factor represses Smad-mediated myofibroblast activation in aortic valvular interstitial cells. FASEB J 22(6):1769-1777.
9. Davis GE, Senger DR. 2005. Endothelial extracellular matrix biosynthesis, remodeling, and functions during vascular morphogenesis and neovessel stabilization. Circ Res 97(11):1093-1107.
10. Derby B. 2010. Inkjet printing of functional and structural materials: Fluid property requirements, feature stability, and resolution. Annu Rev Mater Res 40:395-414.
11. Derby B. 2012. Printing and prototyping of tissues and scaffolds. Science 338(6109):921-926.
12. Duan B, Kapetanovic E, Hockaday LA, Butcher JT. 2014. Three-dimensional printed trileaflet valve conduits using biological hydrogels and human valve interstitial cells. Acta Biomater 10(5):1836-1846.
13. Duffy DC, McDonald JC, Schueller OJA, Whitesides GM. 1998. Rapid prototyping of microfluidic systems in poly (dimethylsiloxane). Anal Chem 70(23):4974-4984.
14. Ertl P, Sticker D, Charwat V, Kasper C, Lepperdinger G. 2014. Lab-on-a-chip technologies for stem cell analysis. Trends Biotechnol 32(5):245-253.
15. Fairbanks BD, Singh SP, Bowman CN, Anseth KS. 2011. Photodegradable, photoadaptable hydrogels via radical-mediated disulfide fragmentation reaction. Macromolecules 44(8):2444-2450.
16. Fernandes TG, Kwon SJ, Bale SS, Lee MY, Diogo MM, Clark DS, Cabral JM, Dordick JS. 2010. Three-dimensional cell culture microarray for high-throughput studies of stem cell fate. Biotechnol Bioeng 106(1):106-118.
17. Figeys D, Pinto D. 2000. Lab-on-a-chip: A revolution in biological and medical sciences. Anal Chem 72(9):330A-335A.
18. Flachsbart BR, Wong K, Iannacone JM, Abante EN, Vlach RL, Rauchfuss PA, Bohn PW, Sweedler JV, Shannon MA. 2006. Design and fabrication of a multilayered polymer microfluidic chip with nanofluidic interconnects via adhesive contact printing. Lab Chip 6(5):667-674.
19. Franzesi GT, Ni B, Ling Y, Khademhosseini A. 2006. A controlled-release strategy for the generation of cross-linked hydrogel microstructures. J Am Chem Soc 128(47):15064-15065.
20. Fraser JK, Schreiber R, Strem B, Zhu M, Alfonso Z, Wulur I, Hedrick MH. 2006. Plasticity of human adipose stem cells toward endothelial cells and cardiomyocytes. Nat Clin Pract Cardiovasc Med 3(1 suppl) S33.
21. Gimble JM, Katz AJ, Bunnell BA. 2007. Adipose-derived stem cells for regenerative medicine. Circ Res 100(9):1249-1260.
22. Ginebra MP, Espanol M, Montufar EB, Perez RA, Mestres G. 2010. New processing approaches in calcium phosphate cements and their applications in regenerative medicine. Acta Biomater 6(8):2863-2873.
23. Glicklis R, Shapiro L, Agbaria R, Merchuk JC, Cohen S. 2000. Hepatocyte behavior within three-dimensional porous alginate scaffolds. Biotechnol Bioeng 67(3):344-353.
24. Haraldsson KT, Hutchison JB, Sebra RP, Good BT, Anseth KS, Bowman CN. 2006. 3D polymeric microfluidic device fabrication via contact liquid photolithographic polymerization (CLiPP). Sens Actuators B Chem 113(1):454-460.
25. Hasan A, Paul A, Vrana NE, Zhao X, Memic A, Hwang YS, Dokmeci MR, Khademhosseini A. 2014. Microfluidic techniques for development of 3D vascularized tissue. Biomaterials 35(26):7308-7325.
26. He K, Wang X. 2011. Rapid prototyping of tubular polyurethane and cell/hydrogel constructs. J Bioact Compat Polym 26(4):363-374.
27. Horrocks MH, Rajah L, Jönsson P, Kjaergaard M, Vendruscolo M, Knowles TP, Klenerman D. 2013. Single-molecule measurements of transient biomolecular complexes through microfluidic dilution. Anal Chem 85(14):6855-6859.
28. Huang Y, He K, Wang X. 2013. Rapid prototyping of a hybrid hierarchical polyurethane-cell/hydrogel construct for regenerative medicine. Mater Sci Eng C Mater 33(6):3220-3229.
29. Huang Y, Yuan J, Righi E, Kamoun WS, Ancukiewicz M, Nezivar J, Santosuosso M, Martin JD, Martin MR, Vianello F, Leblanc P, Munn LL, Huang P, Duda DG, Fukumura D, Jain RK, Poznansky MC. 2012. Vascular normalizing doses of antiangiogenic treatment reprogram the immunosuppressive tumor microenvironment and enhance immunotherapy. Proc Natl Acad Sci USA 109(43):17561-17566.
30. Hughes CCW. 2008. Endothelial-stromal interactions in angiogenesis. Curr Opin Hematol 15(3):204-209.
31. Huh D, Hamilton GA, Ingber DE. 2011. From 3D cell culture to organs-on-chips. Trends Cell Biol 21(12):745-754.
32. Hunt JA. 2008. Regenerative medicine: Materials in a cellular world. Nat Mater 7(8):617-618.
33. Hwang H, Park J, Shin C, Do Y, Cho YK. 2013. Three dimensional multicellular co-cultures and anti-cancer drug assays in rapid prototyped multilevel microfluidic devices. Biomed Microdevices 15(4):627-634.
34. Jaffe EA. 1977. Endothelial cells and the biology of factor VIII. New Eng J Med 296(7):377-383.
35. Jeong GS, Kwon GH, Kang AR, Jung BY, Park Y, Chung S, Lee SH. 2011. Microfluidic assay of endothelial cell migration in 3D interpenetrating polymer semi-network HA-Collagen hydrogel. Biomed Microdevices 13(4):717-723.
36. Kaigala GV, Ho S, Penterman R, Backhouse CJ. 2007. Rapid prototyping of microfluidic devices with a wax printer. Lab Chip 7(3):384-387.
37. Kamei M, Saunders WB, Bayless KJ, Dye L, Davis GE, Weinstein BM. 2006. Endothelial tubes assemble from intracellular vacuoles in vivo. Nature 442(7101):453-456.
38. Khoshmanesh K, Nahavandi S, Baratchi S, Mitchell A, Kalantar-zadeh K. 2011. Dielectrophoretic platforms for bio-microfluidic systems. Biosens Bioelectron 26(5):1800-1814.
39. Kitson PJ, Rosnes MH, Sans V, Dragone V, Cronin L. 2012. Configurable 3D-printed millifluidic and microfluidic 'lab on a chip' reactionware devices. Lab Chip 12(18):3267-3271.
40. Kloxin AM, Tibbitt MW, Anseth KS. 2010. Synthesis of photodegradable hydrogels as dynamically tunable cell culture platforms. Nat Protoc 5(12):1867-1887.
41. Kolewe ME, Park H, Gray C, Ye X, Langer R, Freed LE. 2013. 3D structural patterns in scalable, elastomeric scaffolds guide engineered tissue architecture. Adv Mater 25(32):4459-4465.
42. Lee KH, Shin SJ, Park Y, Lee SH. 2009. Synthesis of cell-laden alginate hollow fibers using microfluidic chips and microvascularized tissue-engineering applications. Small 5(11):1264-1268.
43. Lee MY, Kumar RA, Sukumaran SM, Hogg MG, Clark DS, Dordick JS. 2008. Three-dimensional cellular microarray for high-throughput toxicology assays. Proc Natl Acad Sci USA 105(1):59-63.
44. Li S, Xiong Z, Wang X, Yan Y, Liu H, Zhang R. 2009. Direct fabrication of a hybrid cell/hydrogel construct by a double-nozzle assembling technology. J Bioact Compat Polym 24(3):249-265.
45. Li S, Yan Y, Xiong Z, Weng C, Zhang R, Wang X. 2009. Gradient hydrogel construct based on an improved cell assembling system. J Bioact Compat Polym 24(1):84-99.
46. Lin CC, Anseth KS. 2011. Cell-cell communication mimicry with poly (ethylene glycol) hydrogels for enhancing β-cell function. Proc Natl Acad Sci USA 108(16):6380-6385.
47. Lin CS, Xin ZC, Deng CH, Ning H, Lin G, Lue TF. 2010. Defining adipose tissue-derived stem cells in tissue and in culture. Histol Histopathol 25(6):807-815.
48. Liu L, Zhou X, Xu Y, Zhang W, Liu C-H, Wang X. 2015. Controlled release of growth factors for regenerative medicine. Curr Pharm Des 21(12):1627-1632.
49. Liu P, Meagher RJ, Light YK, Yilmaz S, Chakraborty R, Arkin AP, Hazen TC, Singh AK. 2011. Microfluidic fluorescence in situ hybridization and flow cytometry (μFlowFISH). Lab Chip 11(16):2673-2679.
50. Melchels FPW, Domingos MAN, Klein TJ, Malda J, Bartolo PJ, Hutmacher DW. 2012. Additive manufacturing of tissues and organs. Prog Polym Sci 37(8):1079-1104.
51. Miller JS, Stevens KR, Yang MT, Baker BM, Nguyen DH, Cohen DM, Toro E, Chen AA, Galie PA, Yu X, Chaturvedi R, Bhatia SN, Chen CS. 2012. Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues. Nat Mater 11(9):768-774.
52. Northrup MA. 2004. Microfluidics: A few good tricks. Nat Mater 3(5):282-283.
53. Novosel EC, Kleinhans C, Kluger PJ. 2011. Vascularization is the key challenge in tissue engineering. Adv Drug Deliver Rev 63(4-5):300-311.
54. Oh K, Chung JH, Devasia S, Riley JJ. 2009. Bio-mimetic silicone cilia for microfluidic manipulation. Lab Chip 9(11):1561-1566.
55. Pearce JM. 2012. Building research equipment with free, open-source hardware. Science 337(6100):1303-1304.
56. Planat-Benard V, Silvestre JS, Cousin B, André M, Nibbelink M, Tamarat R, Clergue M, Manneville C, Saillan-Barreau C, Duriez M, Tedgui A, Levy B, Pénicaud L, Casteilla L. 2004. Plasticity of human adipose lineage cells toward endothelial cells physiological and therapeutic perspectives. Circulation 109(5):656-663.
57. Qiu L, Wang J, Wen X, Wang H, Wang Y, Lin Q, Du Z, Duan C, Wang C, Wang C. 2012. Transplantation of co-microencapsulated hepatocytes and HUVECs for treatment of fulminant hepatic failure. Int J Artif Organs 35(6):458-465.
58. Rahaman MN, Mao JJ. 2005. Stem cell-based composite tissue constructs for regenerative medicine. Biotechnol Bioeng 91(3):261-284.
59. Reed HA, White CE, Rao V, Allen SAB, Henderson CL, Kohl PA. 2001. Fabrication of microchannels using polycarbonates as sacrificial materials. J Micromech Microeng 11(6):733-737.
60. Resnick N, Yahav H, Shay-Salit A, Shushy M, Schubert S, Zilberman LC, Wofovitz E. 2003. Fluid shear stress and the vascular endothelium: For better and for worse. Prog Biophys Mol Bio 81(3):177-199.
61. Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R. 2010. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells. Nature 468(7325):824-828.
62. Richardson TP, Peters MC, Ennett AB, Mooney DJ. 2001. Polymeric system for dual growth factor delivery. Nat Biotechnol 19(11):1029-1034.
63. Risau W. 1997. Mechanisms of angiogenesis. Nature 386(6626):671-674.
64. Rydholm AE, Reddy SK, Anseth KS, Bowman CN. 2006. Controlling network structure in degradable thiol-acrylate biomaterials to tune mass loss behavior. Biomacromolecules 7(10):2827-2836.
65. Sehested M, Hou-Jensen K. 1981. Factor VIII related antigen as an endothelial cell marker in benign and malignant diseases. Virchows Arch A Pathol Anat Histol 391(2):217-225.
66. Sengenès C, Lolmède K, Zakaroff-Girard A, Busse R, Bouloumié A. 2005. Preadipocytes in the human subcutaneous adipose tissue display distinct features from the adult mesenchymal and hematopoietic stem cells. J Cell Physiol 205(1):114-122.
67. Soto-Gutiérrez A, Navarro-Alvarez N, Zhao D, Rivas-Carrillo JD, Lebkowski J, Tanaka N, Fox IJ, Kobayashi N. 2007. Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Nat Protoc 2(2):347-356.
68. Stratman AN, Malotte KM, Mahan RD, Davis MJ, Davis GE. 2009. Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation. Blood 114(24):5091-5101.
69. Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, Zhang RR, Ueno Y, Zheng YW, Koike N, Aoyama S, Adachi Y, Taniguchi H. 2013. Vascularized and functional human liver from an iPSC-derived organ bud transplant. Nature 499(7459):481-484.
70. Thorsen T, Maerkl SJ, Quake SR. 2002. Microfluidic large-scale integration. Science 298(5593):580-584.
71. Tibbitt MW, Anseth KS. 2009. Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnol Bioeng 103(4):655-663.
72. Tibbitt MW, Anseth KS. 2012. Dynamic microenvironments: the fourth dimension. Sci Transl Med 4(160):160ps24-160ps24.
73. Verhulsela M, Vignesa M, Descroixa S, Malaquina L, Vignjevicb DM, Viovya JL. 2014. A review of microfabrication and hydrogel engineering for micro-organs on chips. Biomaterials 35(6):1816-1832.
74. Villar G, Graham AD, Bayley H. 2013. A tissue-like printed material. Science 340(6128):48-52.
75. Wagers AJ. 2012. The stem cell niche in regenerative medicine. Cell Stem Cell 10(4):362-369.
76. Wang L, Li PC. 2011. Microfluidic DNA microarray analysis: a review. Anal Chim Acta 687(1):12-27.
77. Wang X, He K, Zhang W. 2013. Optimizing the fabrication processes for manufacturing a hybrid hierarchical polyurethane-cell/hydrogel construct. J Bioact Compat Polym 28(4):303-319.
78. Wang X, Tuomi J, Mäkitie AA, Paloheimo K-S, Partanen J, Yliperttula M. 2013. The integrations of biomaterials and rapid prototyping techniques for intelligent manufacturing of complex organs. In: Pignatello R, editor . Advances in Biomaterials Science and Biomedical Applications. Croatia: InTech. p 437-463.
79. Wang X, Wang J. 2015. Vascularization and adipogenesis of a spindle hierarchical adipose-derived stem cell/collagen/alginate-PLGA construct for breast manufacturing. IJITEE 4(8):1-8.
80. Wang X, Xu H. 2010. Incorporation of DMSO and dextran-40 into a gelatin/alginate hydrogel for controlled cell cryopresergation Cryobiology 61(3):345-351.
81. Wang X, Yan Y, Pan Y, Xiong Z, Liu H, Cheng J, Liu F, Lin F, Wu R, Zhang R, Lu Q. 2006. Generation of three-dimensional hepatocyte/gelatin structures with rapid prototyping system. Tissue Eng 12(1):83-90.
82. Wang X, Yan Y, Zhang R. 2007. Rapid prototyping as a tool for manufacturing bioartificial livers. Trends Biotechnol 25(11):505-513.
83. Wang X, Yan Y, Zhang R. 2009. Recent trends and challenges in complex organ manufacturing. Tissue Eng Part B Rev 16(2):189-197.
84. Wang X, Yan Y, Zhang R. 2010. Gelatin-based hydrogels for controlled cell assembly. In: Ottenbrite RM, Park K, Okano T, editors. Biomedical Applications of Hydrogels Handbook. New York: Springer. p 269-284.
85. Wang X, Zhang Q. 2011. Overview on "Chinese-Finnish workshop on biomanufacturing and evaluation techniques". Artif Organs 35(10):E191.
86. Wang X. 2012. Intelligent freeform manufacturing of complex organs. Artif Organs 36(11):951-961.
87. Wang X. 2013. Overview on biocompatibilities of implantable biomaterials. In: Pignatello R, editor . Advances in Biomaterials Science and Biomedical Applications. Croatia: InTech. PL p 111-155.
88. Wang X. 2014. Spatial effects of stem cell engagement in 3D printing constructs. J Stem Cells Res Rev Rep 1(2):5-9.
89. Wang X. 3D printing of tissue/organ scaffolds for regenerative medicine. Handbook of Intelligent Scaffolds for Regenerative Medicine, 2nd Edition. Pan Stanford Publishing, (Book), in press.
90. Wilson JL, McDevitt TC. 2013. Stem cell microencapsulation for phenotypic control, bioprocessing, and transplantation. Biotechnol Bioeng 110(3):667-682.
91. Xu M, Wang X, Yan Y, Yao R, Ge Y. 2010. An cell-assembly derived physiological 3D model of the metabolic syndrome, based on adipose-derived stromal cells and a gelatin/alginate/fibrinogen matrix. Biomaterials 31(14):3868-3877.
92. Xu M, Yan Y, Liu H, Yao R, Wang X. 2009. Controlled adipose-derived stromal cells differentiation into adipose and endothelial cells in a 3D structure established by cell-assembly technique. J Bioact Compat Polym 24(1 suppl):31-47.
93. Xu W, Wang X, Yan Y, Zhang R. 2008. Rapid prototyping of polyurethane for the creation of branched vascular systems. J Bioact Compat Polym 23(2):103-114.
94. Xu W, Wang X, Yan Y, Zhang R. 2008. A polyurethane-gelatin hybrid construct for the manufacturing of implantable bioartificial livers. J Bioact Compat Polym 23(5):409-422.
95. Xu W, Wang X, Yan Y, Zheng W, Xiong Z, Lin F, Wu R, Zhang R. 2007. Rapid prototyping of three-dimensional cell/gelatino/fibrinogen constructs for medical regeneration. J Bioact Compat Polym 22(4):363-377.
96. Xu Y, Wang X. 2015. Application of 3D biomimetic models for drug delivery and regenerative medicine. Curr Pharm Des 21(12):1618-1626.
97. Yamamoto K, Takahashi T, Asahara T, Ohura N, Sokabe T, Kamiya A, Ando J. 2003. Proliferation, differentiation, and tube formation by endothelial progenitor cells in response to shear stress. J Appl Physiol 95(5):2081-2088.
98. Yan Y, Wang X, Pan Y, Liu H, Cheng J, Xiong Z, Lin F, Wu R, Zhang R, Lu Q. 2005. Fabrication of viable tissue-engineered constructs with 3D cell-assembly technique Biomaterials 26(29):5864-5871.
99. Yan Y, Wang X, Xiong Z, Liu H, Liu F, Lin F, Wu R, Zhang R, Lu Q. 2005. Direct construction of a three-dimensional structure with cells and hydrogel. J Bioact Compat Polym 20(3):259-269.
100. Yang C, Tibbitt MW, Basta L, Anseth KS. 2014. Mechanical memory and dosing influence stem cell fate. Nat Mater 13(6):645-652.
101. Yao R, Zhang R, Yan Y, Wang X. 2009. In vitro angiogenesis of 3D tissue engineered adipose tissue. J Bioact Compat Polym 24(1):5-24.
102. Zhao X, Liu L, Wang J, Xu Y, Zhang W, Khang G, Wang X. 2014. In vitro vascularization of a combined system based on a 3D printing technique. J Tissue Eng Regen Med 10.1002/term.1863
103. Zhao X, Wang X. 2013. Preparation of an adipose-derived stem cell/fibrin-poly (d, l-lactic-co-glycolic acid) construct based on a rapid prototyping technique. J Bioact Compat Polym 28(3):191-203.
104. Zheng S, Lin HK, Lu B, Williams A, Datar R, Cote RJ, Tai YC. 2011. 3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood. Biomed Microdevices 13(1):203-213.