Three-dimensional cell culture microarray for high-throughput studies of stem cell fate

Biotechnology and Bioengineering

Volumn 106, Issue 1, 2010, Pages 106-118

  Fernandes, Tiago G ^a,b   Kwon, Seok Joon ^a   Bale, Shyam Sundhar ^a   Lee, Moo Yeal ^c   Diogo, Maria Margarida ^b   Clark, Douglas S ^d   Cabral, Joaquim M S ^b   Dordick, Jonathan S ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^c Solidus Biosciences Inc   (United States)

^d University of California   (United States)

Author keywords

3D cell culture; High throughput; Microarrays; Stem cells; Studies of stem cell fate

Indexed keywords

3-D CELL CULTURE; ALGINATE GEL; CELL MARKERS; CELLULAR MECHANISMS; CELLULAR MICROARRAYS; CELLULAR RESPONSE; DEPOSIT CELLS; EMBRYONIC STEM CELLS; ES CELLS; FIBROBLAST GROWTH FACTOR; FUNCTIONALIZED; GFP REPORTER SYSTEM; GLASS SLIDES; GLASS SURFACES; HIGH THROUGHPUT; HIGH-THROUGHPUT SCREENING; IMMUNOFLUORESCENCE TECHNIQUES; IN-SITU; NEURAL MARKERS; NEW TOOLS; PLURIPOTENCY; PLURIPOTENT; PRECURSOR CELLS; RAPID IDENTIFICATION; RAPID SCREENING; STEM CELL; STEM CELL EXPANSION; THREE DIMENSIONAL CELL CULTURE; THREE-DIMENSIONAL (3D);

CELL CULTURE; GLASS; MICROARRAYS; THREE DIMENSIONAL; THROUGHPUT; TISSUE;

STEM CELLS;

ALGINIC ACID; FIBROBLAST GROWTH FACTOR 4; GREEN FLUORESCENT PROTEIN; RETINOIC ACID; TRANSCRIPTION FACTOR SOX1;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL ENCAPSULATION; CELL FATE; CONTROLLED STUDY; EMBRYONIC STEM CELL; GEL; HIGH THROUGHPUT SCREENING; IMMUNOFLUORESCENCE; MICROARRAY ANALYSIS; MOUSE; NEURAL STEM CELL; NONHUMAN; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; REPORTER GENE; STEM CELL;

ALGINATES; ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CULTURE MEDIA; FIBROBLAST GROWTH FACTOR 4; GELS; GLASS; MICE; MICROARRAY ANALYSIS; SOXB1 TRANSCRIPTION FACTORS; STEM CELLS;

EID: 77951583526     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22661     Document Type: Article

References (38)

1. Anderson DG, Levenberg S, Langer R. 2004. Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells. Nat Biotechnol 22:863-866.
2. Anderson DG, Putnam D, Lavik EB, Mahmood TA, Langer R. 2005. Biomaterial microarrays: Rapid, microscale screening of polymer-cell interaction. Biomaterials 26:4892-4897.
3. Bailey SN, Sabatini DM, Stockwell BR. 2004. Microarrays of small molecules embedded in biodegradable polymers for use in mammalian cell-based screens. Proc Natl Acad Sci USA 101:16144-16149.
4. Castel D, Pitaval A, Debily M-A, Gidrol X. 2006. Cell micrroarrays in drug discovery. Drug Discov Today 11(13/14):616-622.
5. Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, Smith A. 2003. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113:643-655.
6. Davey RE, Zandstra PW. 2006. Spatial organization of embryonic stem cell responsiveness to autocrine Gp130 ligands reveals an autoregulatory stem cell niche. Stem Cells 24:2538-2548.
7. Dellatore S, Garcia A, Miller W. 2008. Mimicking stem cell niches to increase stem cell expansion. Curr Opin Biotechnol 19:534-540.
8. Dike LE, Chen CS, Mrksich M, Tien J, Whitesides GM, Ingber DE. 1999. Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cell Dev Biol Anim 35:441-448.
9. Diogo MM, Henrique D, Cabral JMS. 2008. Optimization and neural commitment of mouse embryonic stem cells. Biotechnol Appl Biochem 49:105-112.
10. Engler AJ, Sen S, Sweeney HL, Discher DE. 2006. Matrix elasticity directs stem cell lineage specification. Cell 126:677-689.
11. Even-Ram S, Artym V, Yamada KM. 2006. Matrix control of stem cell fate. Cell 126:645-647. (Pubitemid 44233640)
12. Fernandes AM, Fernandes TG, Diogo MM, da Silva CL, Henrique D, Cabral JMS. 2007. Mouse embryonic stem cell expansion in a microcarrierbased stirred culture system. J Biotechnol 132:227-236. (Pubitemid 47599708)
13. Fernandes TG, Kwon SJ, Lee M-Y, Clark DS, Cabral JMS, Dordick JS. 2008. On-chip, cell-based microarray immunofluorescence assay for highthroughput analysis of target proteins. Anal Chem 80:6633-6639.
14. Fernandes TG, Diogo MM, Clark DS, Dordick JS, Cabral JMS. 2009. Highthroughput cellular microarray platforms: Applications in drug discovery, toxicology and stem cell research. Trends Biotechnol 27:342-349.
15. Fernandes TG, Fernandes-Platzgummer AM, da Silva CL, Diogo MM, Cabral JMS. 2010. Kinetic and metabolic analysis of mouse embryonic stem cell expansion under serum-free conditions. Biotechnol Lett 32:171-179.
16. Flaim CJ, Chien S, Bhatia SN. 2005. An extracellular matrix microarray for probing cellular differentiation. Nat Methods 2(2):119-125. (Pubitemid 41131033)
17. Flaim CJ, Teng D, Chien S, Bhatia SN. 2008. Combinatorial signaling microenvironments for studying stem cell fate. Stem Cells Dev 17:29-39. (Pubitemid 351271611)
18. Ha J, Engler CR, Lee SJ. 2008. Determination of diffusion coefficients and diffusion characteristics for chlorferon and diethylthiophosphate in Ca-alginate gel beads. Biotechnol Bioeng 100:698-706. (Pubitemid 351913997)
19. Horning JL, Sahoo SK, Vijayaraghavalu S, Dimitrijevic S, Vasir JK, Jain TK, Panda AK, Labhasetwar V. 2008. 3-D tumor model for in vitro evaluation of anticancer drugs. Mol Pharm 5:849-862.
20. Hubbel JA. 2004. Biomaterials science and high-throughput screening. Nat Biotechnol 22:828-829.
21. Khademhosseini A, Langer R, Borenstein J, Vacanti J. 2006. Microscale technologies for tissue engineering and biology. Proc Natl Acad Sci USA 108(8):2480-2487. (Pubitemid 43306575)
22. Kim M, Habiba A, Doherty J, Mills J, Mercer R, Huettner J. 2009. Regulation of mouse embryonic stem cell neural differentiation by retinoic acid. Dev Biol 328:456-471.
23. Klimanskaya I, Rosenthal N, Lanza R. 2008. Derive and conquer: Sourcing and differentiating stem cells for therapeutic applications. Nat Rev Drug Discov 7:131-142. (Pubitemid 351194061)
24. Ko IK, Kato K, Iwata H. 2005. Parallel analysis of multiple surface markers expressed on rat neural stem cells using antibody microarrays. Biomaterials 26:4882-4891. (Pubitemid 40343036)
25. Kwon SJ, Lee M-Y, Ku B, Sherman DH, Dordick JS. 2007. High-throughput, microarray-based synthesis of natural product analogues via in vitro methabolic pathway construction. ACS Chem Biol 2:419-425. (Pubitemid 350001563)
26. Lee M-Y, Park CB, Dordick JS, Clark DS. 2005. Metabolizing enzyme toxicology assay chip (MetaChip) for high-throughput microscale toxicity analyses. Proc Natl Acad Sci USA 102(4):983-987. (Pubitemid 40170712)
27. Lee M-Y, Kumar RA, Sukumaran SM, Hogg MG, Clark DS, Dordick JS. 2008. Three-dimensional cellular microarrays for high-throughput toxicology assays. Proc Natl Acad Sci USA 105(1):59-63.
28. McNeish J. 2004. Embryonic stem cells in drug discovery. Nat Rev Drug Discov 3:70-80. (Pubitemid 38088699)
29. Morrison S, Spradling A. 2008. Stem cells and niches: Mechanisms that promote stem cell maintenance throughout life. Cell 132:598-611. (Pubitemid 351253690)
30. Niwa H, Miyazaki J-I, Smith AG. 2000. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24:372-376. (Pubitemid 30187435)
31. Oyaas J, Storrø I, Svendsen H, Levine DW. 1995. The effective diffusion coefficient and the distribution constant for small molecules in calcium-alginate gel beads. Biotechnol Bioeng 47:492-500.
32. Siti-Ismail N, Bishop AE, Polak JM, Mantalaris A. 2008. The benefit of human embryonic stem cell encapsulation for prolonged feeder-free maintenance. Biomaterials 29:3946-3952.
33. Smith AG. 2001. Embryo-derived stem cells of mice and men. Annu Rev Cell Dev Biol 17:435-462. (Pubitemid 33096183)
34. Soen Y, Mori A, Palmer TD, Brown PO. 2006. Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments. Mol Syst Biol 2:37. (Pubitemid 46061052)
35. Xu Y, Shi Y, Ding S. 2008. A chemical approach to stem-cell biology and regenerative medicine. Nature 453:338-344. (Pubitemid 351693140)
36. Ying Q-L, Nichols J, Chambers I, Smith A. 2003a. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115:281-292. (Pubitemid 37487702)
37. Ying Q-L, Stavridis M, Griffiths D, Li M, Smith A. 2003b. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol 21(2):183-186. (Pubitemid 36188218)
38. Ying Q-L, Wray J, Nichols J, Batlle-Morera L, Doble B, Woodgett J, Cohen P, Smith A. 2008. The ground state of embryonic stem cell self-renewal. Nature 453:519-523. (Pubitemid 351733324)