Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system

Biotechnology and Bioengineering

Volumn 112, Issue 8, 2015, Pages 1663-1672

  Chemla, Yonatan ^a   Ozer, Eden ^a   Schlesinger, Orr ^a   Noireaux, Vincent ^b   Alfonta, Lital ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

^b University of Minnesota   (United States)

Author keywords

Amber suppression; Cell free protein synthesis; Genetic code expansion; Pyrrolysyl tRNA synthetase

Indexed keywords

AMINO ACIDS; CELLS; CYTOLOGY; PROTEINS;

AMBER SUPPRESSIONS; CELL FREE PROTEIN SYNTHESIS; CELL-FREE REACTIONS; GENETIC CODE; RIBOSOMAL TRANSLATION; SYNTHETASES; TRANSLATION SYSTEMS; UNNATURAL AMINO ACIDS;

BIOSYNTHESIS;

AMINO ACID TRANSFER RNA LIGASE; GREEN FLUORESCENT PROTEIN; AMINO ACID; TRANSFER RNA;

ARTICLE; BACTERIAL STRAIN; BIOENGINEERING; CELL FREE SYSTEM; CLICK CHEMISTRY; CYCLOADDITION; CYTOLYSIS; ENERGY RESOURCE; ESCHERICHIA COLI; GENETIC CODE; IN VITRO STUDY; METHANOSARCINA MAZEI; NONHUMAN; PROTEIN EXPRESSION; PROTEIN STABILITY; PROTEIN SYNTHESIS; STOP CODON; GENETICS; METABOLISM; PROCEDURES; PROTEIN ENGINEERING; RIBOSOME;

AMINO ACIDS; AMINO ACYL-TRNA SYNTHETASES; CELL-FREE SYSTEM; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RIBOSOMES; RNA, TRANSFER;

EID: 84932197247     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25587     Document Type: Article

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