In vitro evolution of α-hemolysin using a liposome display

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 42, 2013, Pages 16796-16801

  Fujii, Satoshi ^a   Matsuura, Tomoaki ^a,b   Sunami, Takeshi ^a   Kazuta, Yasuaki ^a   Yomo, Tetsuya ^a,b  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Directed evolution; FACS; Giant unilamellar vesicles; In vitro synthetic biology; Pure system

Indexed keywords

ALPHA HEMOLYSIN; LIGAND; LIPOSOME; MUTANT PROTEIN;

ARTICLE; AUTORADIOGRAPHY; BINDING AFFINITY; CLONE; COMPARATIVE STUDY; CONTROLLED STUDY; DNA LIBRARY; ENCAPSULATION; EVOLUTION; GENE POOL; IN VITRO STUDY; LIPID COMPOSITION; MEMBRANE BINDING; NONHUMAN; PHOSPHOLIPID MEMBRANE; PHOSPHOLIPID VESICLE; POINT MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EVOLUTION; PROTEIN EXPRESSION; PROTEIN STABILITY; PROTEIN SYNTHESIS; STAPHYLOCOCCUS AUREUS;

DIRECTED EVOLUTION; FACS; GIANT UNILAMELLAR VESICLES; IN VITRO SYNTHETIC BIOLOGY; PURE SYSTEM;

BACTERIAL TOXINS; DIRECTED MOLECULAR EVOLUTION; HEMOLYSIN PROTEINS; LIPOSOMES; PHOSPHOLIPIDS; POINT MUTATION; STAPHYLOCOCCUS AUREUS;

EID: 84885737601     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1314585110     Document Type: Article

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