Artificial extracellular matrix proteins containing phenylalanine analogues biosynthesized in bacteria using T7 expression system and the PEGylation

Biomacromolecules

Volumn 12, Issue 10, 2011, Pages 3444-3452

  Takasu, Akinori ^a   Kondo, Shiori ^a   Ito, Akihiro ^a   Furukawa, Yuya ^a   Higuchi, Masahiro ^a   Kinoshita, Takatoshi ^a   Kwon, Inchan ^b  

^a NAGOYA INSTITUTE OF TECHNOLOGY   (Japan)

^b California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTIFICIAL EXTRACELLULAR MATRIX PROTEINS; BINDING POCKETS; CIRCULAR DICHROMISM; CONFORMATIONAL CHANGE; CRITICAL TRANSITION TEMPERATURES; CULTURE CONDITIONS; EXPRESSION LEVELS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; IN-VIVO; INCORPORATION RATIO; MATERIAL SCIENCE; PEGYLATION; RANDOM COIL; REACTION SITES; RNA POLYMERASE; SYNTHETASES; T7 EXPRESSION SYSTEM; TURN STRUCTURE;

AMINO ACIDS; BACTERIOLOGY; CELL CULTURE; ENDOTHELIAL CELLS; GLYCOLS; HYDROPHILICITY; POLYETHYLENE GLYCOLS; RNA; SYNTHESIS (CHEMICAL); TURBIDITY;

PROTEINS;

ALANINE; ELASTIN; FIBRONECTIN; GLYCINE; MACROGOL; MUTANT PROTEIN; P ACETYLPHENYLALANINE; P AZIDOPHENYLALANINE; P IODOPHENYLALANINE; PHENYLALANINE DERIVATIVE; PHENYLALANINE TRANSFER RNA LIGASE; RNA POLYMERASE; THREONINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ARTIFICIAL EXTRACELLULAR MATRIX; BIOSYNTHESIS; CELL ADHESION; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CULTURE MEDIUM; ESCHERICHIA COLI; EXTRACELLULAR MATRIX; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; HYDROPHILICITY; LOW TEMPERATURE; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; T7 EXPRESSION SYSTEM; TRANSITION TEMPERATURE; TURBIDITY; UMBILICAL VEIN ENDOTHELIAL CELL;

BINDING SITES; CELL ADHESION; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; EXTRACELLULAR MATRIX PROTEINS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; PHENYLALANINE; PHENYLALANINE-TRNA LIGASE; PLASMIDS; POLYETHYLENE GLYCOLS; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; RNA, TRANSFER, PHE; TISSUE ENGINEERING; TRANSFORMATION, BACTERIAL; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 80053948970     PISSN: 15257797     EISSN: 15264602     Source Type: Journal    
DOI: 10.1021/bm200522d     Document Type: Article

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