Humanization of a phosphothreonine peptide-specific chicken antibody by combinatorial library optimization of the phosphoepitope-binding motif

Biochemical and Biophysical Research Communications

Volumn 463, Issue 3, 2015, Pages 414-420

  Baek, Du San ^a   Kim, Yong Sung ^a  

^a Ajou University   (South Korea)

Author keywords

Antibody engineering; Chicken antibody; Humanization; Phosphospecific antibody; Phosphothreonine binding motif

Indexed keywords

ANTIBODY; CHICKEN ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN G1; PHOSPHOSPECIFIC ANTIBODY; PHOSPHOTHREONINE; TAU PROTEIN; UNCLASSIFIED DRUG; IMMUNOGLOBULIN VARIABLE REGION; MONOCLONAL ANTIBODY;

ALLOSTERISM; ANTIBODY ENGINEERING; ANTIBODY LIBRARY; ANTIBODY SCREENING; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; COMBINATORIAL LIBRARY; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DIPLOIDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE LIBRARY; HAPLOIDY; HUMAN; HUMAN CELL; HUMANIZATION; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; REVERTANT; YEAST CELL; AMINO ACID SEQUENCE; ANIMAL; ANTIBODY AFFINITY; CHEMICAL STRUCTURE; CHEMISTRY; CHICKEN; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; MOLECULAR GENETICS; PROTEIN ENGINEERING;

ANIMALIA;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL, HUMANIZED; ANTIBODY AFFINITY; CHICKENS; GENE LIBRARY; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHOSPHOTHREONINE; PROTEIN ENGINEERING; TAU PROTEINS;

EID: 84930932414     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2015.05.086     Document Type: Article

References (26)

1. H. Nishi, A. Shaytan, and A.R. Panchenko Physicochemical mechanisms of protein regulation by phosphorylation Front. Genet. 5 2014 270
2. C.S. Tan Sequence, structure, and network evolution of protein phosphorylation Sci. Signal. 4 2011 mr6
3. D. Hanahan, and R.A. Weinberg Hallmarks of cancer: the next generation Cell 144 2011 646 674
4. H. Goto, and M. Inagaki Production of a site- and phosphorylation state-specific antibody Nat. Protoc. 2 2007 2574 2581
5. H.H. Shih, C. Tu, and W. Cao, et al., An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition J. Biol. Chem. 287 2012 44425 44434
6. J.T. Koerber, N.D. Thomsen, and B.T. Hannigan, et al., Nature-inspired design of motif-specific antibody scaffolds Nat. Biotechnol. 31 2013 916 921
7. C.A. Kettleborough, J. Saldanha, and V.J. Heath, et al., Humanization of a mouse monoclonal antibody by CDR-grafting: the importance of framework residues on loop conformation Protein Eng. 4 1991 773 783
8. S.H. Lee, D.W. Park, and E.S. Sung, et al., Humanization of an agonistic anti-death receptor 4 single chain variable fragment antibody and avidity-mediated enhancement of its cell death-inducing activity Mol. Immunol. 47 2010 816 824
9. D.S. Kim, S.H. Lee, and J.S. Kim, et al., Generation of humanized anti-DNA hydrolyzing catalytic antibodies by complementarity determining region grafting Biochem. Biophys. Res. Commun. 379 2009 314 318
10. P.R. Tempest, P. White, and M. Buttle, et al., Identification of framework residues required to restore antigen binding during reshaping of a monoclonal antibody against the glycoprotein gB of human cytomegalovirus Int. J. Biol. Macromol. 17 1995 37 42
11. J. Foote, and G. Winter Antibody framework residues affecting the conformation of the hypervariable loops J. Mol. Biol. 224 1992 487 499
12. N. Nishibori, H. Horiuchi, and S. Furusawa, et al., Humanization of chicken monoclonal antibody using phage-display system Mol. Immunol. 43 2006 634 642
13. E.A. Padlan A possible procedure for reducing the immunogenicity of antibody variable domains while preserving their ligand-binding properties Mol. Immunol. 28 1991 489 498
14. H. Wu, Y. Nie, and W.D. Huse, et al., Humanization of a murine monoclonal antibody by simultaneous optimization of framework and CDR residues J. Mol. Biol. 294 1999 151 162
15. W.F. Dall'Acqua, M.M. Damschroder, and J. Zhang, et al., Antibody humanization by framework shuffling Methods 36 2005 43 60
16. D.K. Choi, J. Bae, and S.M. Shin, et al., A general strategy for generating intact, full-length IgG antibodies that penetrate into the cytosol of living cells mAbs 6 2014 1402 1414
17. D.S. Baek, and Y.S. Kim Construction of a large synthetic human Fab antibody library on yeast cell surface by optimized yeast mating J. Microbiol. Biotechnol. 24 2014 408 420
18. C.H. Lee, K.J. Park, and E.S. Sung, et al., Engineering of a human kringle domain into agonistic and antagonistic binding proteins functioning in vitro and in vivo Proc. Natl. Acad. Sci. U. S. A. 107 2010 9567 9571
19. L. Benatuil, J.M. Perez, and J. Belk, et al., An improved yeast transformation method for the generation of very large human antibody libraries Protein Eng. Des. Sel. 23 2010 155 159
20. T.H. Shin, E.S. Sung, and Y.J. Kim, et al., Enhancement of the tumor penetration of monoclonal antibody by fusion of a neuropilin-targeting peptide improves the antitumor efficacy Mol. Cancer Ther. 13 2014 651 661
21. J. Ko, H. Park, and L. Heo, et al., GalaxyWEB server for protein structure prediction and refinement Nucleic Acids Res. 40 2012 W294 W297
22. E.A. Kabat, T.T. Wu, and H. Perry, et al., Sequences of Proteins of Immunological Interest 1991 NIH Publication No. 91-3142
23. J. Ye, N. Ma, and T.L. Madden, et al., IgBLAST: an immunoglobulin variable domain sequence analysis tool Nucleic Acids Res. 41 2013 W34 W40
24. M. Steinwand, P. Droste, and A. Frenzel,∗ et al., The influence of antibody fragment format on phage display based affinity maturation of IgG mAbs 6 2014 204 218
25. A. Tramontano, C. Chothia, and A.M. Lesk Framework residue 71 is a major determinant of the position and conformation of the second hypervariable region in the VH domains of immunoglobulins J. Mol. Biol. 215 1990 175 182
26. A. Ittner, J. Bertz, and L.S. Suh, et al., Tau-targeting passive immunization modulates aspects of pathology in tau transgenic mice J. Neurochem. 132 2015 135 145