Construction of a large synthetic human Fab antibody library on yeast cell surface by optimized yeast mating

Journal of Microbiology and Biotechnology

Volumn 24, Issue 3, 2014, Pages 408-420

  Baek, Du San ^a   Kim, Yong Sung ^a  

^a Ajou University   (South Korea)

Author keywords

Germline repertoires; Human Fab library; Yeast mating; Yeast surface display

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT;

AMINO ACID COMPOSITION; ANTIBODY LIBRARY; ANTIBODY SCREENING; ANTIBODY SPECIFICITY; ARTICLE; CELL DENSITY; CELL GROWTH; CELL SURFACE DISPLAY; CELL TRANSFORMATION; CODON; COMPLEMENTARITY DETERMINING REGION; HAPLOIDY; HEAVY CHAIN; HUMAN; LIGHT CHAIN; NONHUMAN; PH; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; YEAST CELL; YEAST MATING;

ANTIBODIES; CELL SURFACE DISPLAY TECHNIQUES; COMPLEMENTARITY DETERMINING REGIONS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; MEMBRANE PROTEINS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE;

EID: 84897847392     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1401.01002     Document Type: Article

References (31)

1. Benatuil L, Perez JM, Belk J, Hsieh CM. 2010. An improved yeast transformation method for the generation of very large human antibody libraries. Protein Eng. Des. Sel. 23: 155-159.
2. Blaise L, Wehnert A, Steukers MP, van den Beucken T, Hoogenboom HR, Hufton SE. 2004. Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments. Gene 342: 211-218.
3. Boder ET, Wittrup KD. 1997. Yeast surface display for screening combinatorial polypeptide libraries. Nat. Biotechnol. 15: 553-557.
4. Cappellaro C, Baldermann C, Rachel R, Tanner W. 1994. Mating type-specific cell-cell recognition of Saccharomyces cerevisiae: cell wall attachment and active sites of a-and alpha-agglutinin. EMBO J. 13: 4737-4744.
5. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD. 2006. Isolating and engineering human antibodies using yeast surface display. Nat. Protoc. 1: 755-768.
6. Choi HJ, Kim YJ, Lee S, Kim YS. 2013. A heterodimeric Fcbased bispecific antibody simultaneously targeting VEGFR-2 and Met exhibits potent antitumor activity. Mol. Cancer Ther. 12: 2748-2759.
7. Ewert S, Huber T, Honegger A, Pluckthun A. 2003. Biophysical properties of human antibody variable domains. J. Mol. Biol. 325: 531-553.
8. Feldhaus MJ, Siegel RW, Opresko LK, Coleman JR, Feldhaus JM, Yeung YA, et al. 2003. Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library. Nat. Biotechnol. 21: 163-170.
9. Glanville J, Zhai W, Berka J, Telman D, Huerta G, Mehta GR, et al. 2009. Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire. Proc. Natl. Acad. Sci. USA 106: 20216-20221.
10. Hwang WY, Foote J. 2005. Immunogenicity of engineered antibodies. Methods 36: 3-10.
11. Kabat EA, Wu TT, Perry H, Gottesmann K, Foeller C. 1991. Sequences of Proteins of Immunological Interest. NIH, Publication No. 91-3242.
12. Kim MS, Choi HY, Choi YS, Kim J, Kim YS. 2007. Optimized serological isolation of lung-cancer-associated antigens from a yeast surface-expressed cDNA library. J. Microbiol. Biotechnol. 17: 993-1001.
13. Kim MS, Lee SH, Song MY, Yoo TH, Lee BK, Kim YS. 2007. Comparative analyses of complex formation and binding sites between human tumor necrosis factor-alpha and its three antagonists elucidate their different neutralizing mechanisms. J. Mol. Biol. 374: 1374-1388.
14. Knappik A, Ge L, Honegger A, Pack P, Fischer M, Wellnhofer G, et al. 2000. Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. J. Mol. Biol. 296: 57-86.
15. Lee CH, Park KJ, Kim SJ, Kwon O, Jeong KJ, Kim A, Kim YS. 2011. Generation of bivalent and bispecific kringle single domains by loop grafting as potent agonists against death receptors 4 and 5. J. Mol. Biol. 411: 201-219.
16. Lee CV, Liang WC, Dennis MS, Eigenbrot C, Sidhu SS, Fuh G. 2004. High-affinity human antibodies from phagedisplayed synthetic Fab libraries with a single framework scaffold. J. Mol. Biol. 340: 1073-1093.
17. Lipke PN, Wojciechowicz D, Kurjan J. 1989. AG alpha 1 is the structural gene for the Saccharomyces cerevisiae alphaagglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating. Mol. Cell Biol. 9: 3155-3165.
18. Lou J, Geren I, Garcia-Rodriguez C, Forsyth CM, Wen W, Knopp K, et al. 2010. Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via y east mating. Protein Eng. Des. Sel. 23: 311-319.
19. Marks JD, Hoogenboom HR, Bonnert TP, McCafferty J, Griffiths AD, Winter G. 1991. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222: 581-597.
20. Meilhoc E, Masson JM, Teissie J. 1990. High efficiency transformation of intact yeast cells by electric field pulses. Biotechnology (NY) 8: 223-227.
21. Ponsel D, Neugebauer J, Ladetzki-Baehs K, Tissot K. 2011. High affinity, developability and functional size: the holy grail of combinatorial antibody library generation. Molecules 16: 3675-3700.
22. Romanos MA, Scorer CA, Clare JJ. 1992. Foreign gene expression in yeast: a review. Yeast 8: 423-488.
23. Schroeder HW, Cavacini L Jr. 2010. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125: S41-S52.
24. Sena EP, Radin DN, Fogel S. 1973. Synchronous mating in yeast. Proc. Natl. Acad. Sci. USA 70: 1373-1377.
25. Shen ZM, Wang L, Pike J, Jue CK, Zhao H, de Nobel H, et al. 2001. Delineation of functional regions within the subunits of the Saccharomyces cerevisiae cell adhesion molecule aagglutinin. J. Biol. Chem. 276: 15768-15775.
26. Soellick TR, Uhrig JF. 2001. Development of an optimized interaction-mating protocol for large-scale yeast two-hybrid analyses. Genome Biol. 2: RESEARCH0052.
27. Tiller T, Schuster I, Deppe D, Siegers K, Strohner R, Herrmann T, et al. 2013. A fully synthetic human Fab antibody library based on f ixed VH/VL framework pairings with favorable biophysical properties. MAbs 5: 445-470.
28. Tomlinson IM, Cox JP, Gherardi E, Lesk AM, Chothia C. 1995. The structural repertoire of the human V kappa domain. EMBO J. 14: 4628-4638.
29. Weaver-Feldhaus JM, Lou J, Coleman JR, Siegel RW, Marks JD, Feldhaus MJ. 2004. Yeast mating for combinatorial Fab library generation and surface display. FEBS Lett. 564: 24-34.
30. Yang HY, Kang KJ, Chung JE, Shim H. 2009. Construction of a large synthetic human scFv library with six diversified CDRs and high functional diversity. Mol. Cells 27: 225-235.
31. Zhai W, Glanville J, Fuhrmann M, Mei L, Ni I, Sundar PD, et al. 2011. Synthetic antibodies designed on natural sequence landscapes. J. Mol. Biol. 412: 55-71.