Evaluation of human and non-human primate antibody binding to pig cells lacking GGTA1/CMAH/β4GalNT2 genes

Xenotransplantation

Volumn 22, Issue 3, 2015, Pages 194-202

  Estrada, Jose L ^a   Martens, Greg ^a   Li, Ping ^a   Adams, Andrew ^b,c   Newell, Kenneth A ^c   Ford, Mandy L ^c   Butler, James R ^a   Sidner, Richard ^a   Tector, Matt ^d   Tector, Joseph ^a,e  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b EMORY UNIVERSITY   (United States)

^c EMORY UNIVERSITY   (United States)

^d INDIANA UNIVERSITY HEALTH   (United States)

^e IU Health Transplant Institute   (United States)

Author keywords

antibody; Cas9; CRISPR; genetic engineering; primate; swine; xenoantigen; 4GalNT2

Indexed keywords

ANTIBODY; BETA 1,4 N ACETYLGALACTOSAMINYL TRANSFERASE; CASPASE 9; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; N ACETYLGALACTOSAMINYLTRANSFERASE; RNA; UNCLASSIFIED DRUG; ALPHA-1,3-GALACTOSYLTRANSFERASE 1, PORCINE; CMPACETYLNEURAMINATE MONOOXYGENASE; GALACTOSYLTRANSFERASE; HETEROPHILE ANTIGEN; MIXED FUNCTION OXIDASE; PROTEIN BINDING; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIBODY DETECTION; ANTIGEN BINDING; ARTICLE; BABOON; BETA4 GALNT2 GENE; BINDING AFFINITY; BINDING SITE; CELL ASSAY; CMAH GENE; CONTROLLED STUDY; GENE; GENE FUNCTION; GENE IDENTIFICATION; GGTA1 GENE; MOLECULAR DYNAMICS; NONHUMAN; PIG; PRIORITY JOURNAL; RHESUS MONKEY; ANIMAL; GENETICS; GRAFT REJECTION; HUMAN; IMMUNOLOGY; METABOLISM; MONONUCLEAR CELL; TRANSGENIC ANIMAL; XENOGRAFT;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; ANTIGENS, HETEROPHILE; GALACTOSYLTRANSFERASES; GRAFT REJECTION; HUMANS; LEUKOCYTES, MONONUCLEAR; MIXED FUNCTION OXYGENASES; PROTEIN BINDING; RECOMBINANT PROTEINS; SWINE; TRANSPLANTATION, HETEROLOGOUS;

EID: 84930753871     PISSN: 0908665X     EISSN: 13993089     Source Type: Journal    
DOI: 10.1111/xen.12161     Document Type: Article

References (26)

1. Israni AK, Salkowski N, Gustafson S, et al., New national allocation policy for deceased donor kidneys in the United States and possible effect on patient outcomes. J Am Soc Nephrol 2014; 25: 1842-1848.
2. Chen G, Qian H, Starzl T, et al., Acute rejection is associated with antibodies to non-Gal antigens in baboons using Gal-knockout pig kidneys. Nat Med 2005; 11: 1295-1298.
3. Galili U,. Discovery of the natural anti-Gal antibody and its past and future relevance to medicine. Xenotransplantation 2013; 20: 138-147.
4. Salama A, Evanno G, Harb J, Soulillou J,. Potential deleterious role of anti-Neu5Gc antibodies in xenotransplantation. Xenotransplantation 2015; 22: 85-94.
5. Byrne GW, Stalboerger PG, Davila E, et al., Proteomic identification of non-Gal antibody targets after pig-to-primate cardiac xenotransplantation. Xenotransplantation 2008; 15: 268-276.
6. Li P., Estrada JL, Burlak C, Tector AJ,. Biallelic knockout of the alpha-1,3 galactosyltransferase gene in porcine liver-derived cells using zinc finger nucleases. J Surg Res 2013; 181: e39-e45.
7. Lutz AJ, Li P, Estrada JL, et al., Double knockout pigs deficient in N-glycolylneuraminic acid and galactose alpha-1,3-galactose reduce the humoral barrier to xenotransplantation. Xenotransplantation 2014; 20: 27-35.
8. Tan W, Carlson DF, Lancto CA, et al., Efficient nonmeiotic allele introgression in livestock using custom endonucleases. Proc Natl Acad Sci USA 2014; 110: 16526-16531.
9. Li P, Estrada JL, Burlak C, et al., Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection. Xenotransplantation 2015; 22: 20-31.
10. Reyes LM, Estrada JL, Wang ZY, et al., Creating class I MHC-null pigs using guide RNA and the Cas9 endonuclease. J Immunol 2014; 193: 5751-5757.
11. Sato M, Miyoshi K, Nagao Y, et al., The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the alpha-1,3-galactosyltransferase gene in porcine embryonic fibroblasts. Xenotransplantation 2014; 21: 291-300.
12. Whitworth KM, Lee K, Benne JA, et al., Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos. Biol Reprod 2014; 91: 78.
13. Zhou X, Xin J, Fan N, et al., Generation of CRISPR/tCas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Cell Mol Life Sci 2014; 1-10.
14. Tector AJ, Ford ML,. Literature Watch Implications for transplantation. Am J Transplant 2015; 15: 3.
15. Wang ZY, Burlak C, Estrada JL, et al., Erythrocytes from GGTA1/CMAH knockout pigs: implications for xenotransfusion and testing in non-human primates. Xenotransplantation 2014; 21: 376-384.
16. Burlak C, Paris LL, Lutz AJ, et al., Reduced binding of human antibodies to cells from GGTA1/CMAH KO pigs. Am J Transplant 2014; 14: 1895-1900.
17. Reemtsma K, McCracken BH, Schlegel JU, et al., Renal heterotransplantation in man. Ann Surg 1964; 160: 384-410.
18. Byrne GW, Stalboerger PG, Du Z, Davis TR, McGregor CGA,. Identification of new carbohydrate and membrane protein antigens in cardiac xenotransplantation. Transplantation 2011; 91: 287-292.
19. Byrne GW, Du Z, Stalboerger PG, Kogelberg H, McGregor CGA,. Cloning and expression of porcine beta1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen. Xenotransplantation 2014; 21: 543-554.
20. Cong L, Ran FA, Cox D, et al., Multiplex genome engineering using CRISPR/Cas systems. Science 2013; 339: 819-823.
21. Waghmare SK, Estrada J, Reyes L, et al., Gene targeting and cloning in pigs using fetal liver derived cells. J Surg Res 2011; 171: e223-e229.
22. Estrada J, Sommer J, Collins B, et al., Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR). Cloning Stem Cells 2007; 9: 229-236.
23. Bardor M, Nguyen DH, Diaz S, Varki A,. Mechanism of uptake and incorporation of the non-human sialic acid N-glycolylneuraminic acid into human cells. J Biol Chem 2005; 280: 4228-4237.
24. Calne RY,. Organ transplantation between widely disparate species. Transplant Proc 1970; 2: 550-556.
25. Hardy MA,. Xenograft 25: proceedings of the International Congress, Xenograft 25, held at Arden House, Harriman, New York, 11-13 November 1988. Amsterdam; New York New York, NY, USA, Excerpta Medica; Sole distributors for the USA and Canada, Elsevier Science Pub. Co.
26. Padler-Karavani V, Varki A,. Potential impact of the non-human sialic acid N-glycolylneuraminic acid on transplant rejection risk. Xenotransplantation 2011; 18: 1-5.