Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection

Xenotransplantation

Volumn 22, Issue 1, 2015, Pages 20-31

  Li, Ping ^a   Estrada, Jose L ^a   Burlak, Christopher ^a   Montgomery, Jessica ^a   Butler, James R ^a   Santos, Rafael M ^a   Wang, Zheng Yu ^a   Paris, Leela L ^a   Blankenship, Ross L ^a   Downey, Susan M ^a   Tector, Matthew ^b   Tector, A Joseph ^b  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b INDIANA UNIVERSITY HEALTH   (United States)

Author keywords

CRISPR; genetically modified pigs; IB4 lectin; iGb3S

Indexed keywords

CARBOHYDRATE; GALACTOSE; GUIDE RNA; NUCLEASE; ALPHA-1,3-GALACTOSYLTRANSFERASE 1, PORCINE; CMPACETYLNEURAMINATE MONOOXYGENASE; GALACTOSYLTRANSFERASE; GRIFFONIA SIMPLICIFOLIA LECTINS; HETEROPHILE ANTIGEN; MIXED FUNCTION OXIDASE; PLANT LECTIN; STREPTAVIDIN; UDP-GALACTOSE BETA-D-GALACTOSYL-1,4-GLYCOSYLCERAMIDE ALPHA-1,3-GALACTOSYLTRANSFERASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA MODIFICATION; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; EXPRESSION VECTOR; GENE; GENE DISRUPTION; GENE MUTATION; NONHUMAN; PREGNANCY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SWINE; XENOTRANSPLANTATION; ANIMAL; BIOTINYLATION; CELL NUCLEUS TRANSPLANTATION; CRISPR CAS SYSTEM; CYTOLOGY; FEMALE; GENE DELETION; GENE INACTIVATION; GENE VECTOR; GENETICS; IMMUNOMAGNETIC SEPARATION; LIVER CELL; METABOLISM; PHENOTYPE; PIG;

ANIMALS; ANTIGENS, HETEROPHILE; BIOTINYLATION; CRISPR-CAS SYSTEMS; FEMALE; GALACTOSYLTRANSFERASES; GENE DELETION; GENE KNOCKOUT TECHNIQUES; GENETIC VECTORS; HEPATOCYTES; IMMUNOMAGNETIC SEPARATION; MIXED FUNCTION OXYGENASES; NUCLEAR TRANSFER TECHNIQUES; PHENOTYPE; PLANT LECTINS; PREGNANCY; RNA, GUIDE; STREPTAVIDIN; SUS SCROFA; SWINE;

EID: 84922755847     PISSN: 0908665X     EISSN: 13993089     Source Type: Journal    
DOI: 10.1111/xen.12131     Document Type: Article

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