Human cytochrome P450 21A2, the major steroid 21-hydroxylase: Structure of the enzyme•progesterone substrate complex and rate-limiting C-H bond cleavage

Journal of Biological Chemistry

Volumn 290, Issue 21, 2015, Pages 13128-13143

  Pallan, Pradeep S ^a   Wang, Chunxue ^a   Lei, Li ^a   Yoshimoto, Francis K ^a   Auchus, Richard J ^b   Waterman, Michael R ^a   Guengerich, F Peter ^b   Egli, Martin ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL STRUCTURE; ENZYMES; ISOTOPES;

CATALYTIC EFFICIENCIES; COMPETITIVE KINETICS; CONGENITAL ADRENAL HYPERPLASIAS; DEUTERIUM ISOTOPE EFFECT; EFFICIENT OXIDATIONS; GENETIC DEFICIENCIES; SUBSTRATE COMPLEXES; THREE-DIMENSIONAL STRUCTURE;

SUBSTRATES;

CYTOCHROME P450 21A2; DEUTERIUM; HYDROXYPROGESTERONE; PROGESTERONE; STEROID 21 MONOOXYGENASE; UNCLASSIFIED DRUG; CYP21A2 PROTEIN, HUMAN; PROTEIN BINDING;

ARTICLE; CARBON BOND; CATALYSIS; CHEMICAL BOND; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; GENETIC DISORDER; HORMONE BINDING; HUMAN; HYDROGEN BOND; HYDROXYLATION; KINETICS; OXIDATION; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN CLEAVAGE; STEADY STATE; AMINO ACID SEQUENCE; ANIMAL; BINDING SITE; BOVINE; CHEMISTRY; ENZYME SPECIFICITY; METABOLISM; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

BOVINAE;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; CATTLE; CRYSTALLOGRAPHY, X-RAY; DEUTERIUM; HUMANS; HYDROXYLATION; KINETICS; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PROGESTERONE; PROTEIN BINDING; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; STEROID 21-HYDROXYLASE; SUBSTRATE SPECIFICITY;

EID: 84929991898     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M115.646307     Document Type: Article

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