Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis

Applied Microbiology and Biotechnology

Volumn 98, Issue 9, 2014, Pages 4009-4020

  Dudek, Hanna M ^a   Fink, Michael J ^b   Shivange, Amol V ^c   Dennig, Alexander ^c   Mihovilovic, Marko D ^b   Schwaneberg, Ulrich ^c   Fraaije, Marco W ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

^c RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Baeyer Villiger monooxygenase; Biocatalysis; OmniChange mutagenesis; Oxygenation; Protein engineering

Indexed keywords

KETONES; OXYGENATION;

ACTIVITY DETECTION; ALIPHATIC KETONES; BAEYER-VILLIGER MONOOXYGENASES; BIOCATALYSIS; INDUSTRIAL PROCESSS; MULTIPLE-SITE MUTAGENESIS; MUTANT LIBRARIES; PROTEIN ENGINEERING;

MUTAGENESIS;

ALIPHATIC KETONE; AROMATIC COMPOUND; BAEYER VILLIGER MONOOXYGENASE; CYCLOPENTANONE DERIVATIVE; KETONE; PHENYLACETONE MONOOXYGENASE; PHOSPHATE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; 1-PHENYL-2-PROPANONE; ACETONE; MIXED FUNCTION OXIDASE;

ARTICLE; BAEYER VILLIGER REACTION; BIOTRANSFORMATION; ENZYME STRUCTURE; FLUORESCENCE; KINETICS; MOLECULAR LIBRARY; MULTIPLE SITE MUTAGENESIS; MUTAGENESIS; NONHUMAN; SCREENING; THERMOSTABILITY; ANALOGS AND DERIVATIVES; ENZYME SPECIFICITY; GENETIC SCREENING; GENETICS; METABOLISM;

ACETONE; GENETIC TESTING; KETONES; MIXED FUNCTION OXYGENASES; MUTAGENESIS; SUBSTRATE SPECIFICITY;

EID: 84899956948     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5364-1     Document Type: Article

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