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Volumn 14, Issue , 2015, Pages 111-125

Hurdles and pitfalls in measuring antioxidant efficacy: A critical evaluation of ABTS, DPPH, and ORAC assays

Author keywords

ABTS + 2,2' azino bis(3 ethylbenzothiazoline 6 sulfonic acid) assay; Antioxidant efficacy assays; Limitations; ORAC Oxygen Radical Absorbance Capacity assay; Radical quenching kinetics; TEAC Trolox equivalent antioxidant capacity assay

Indexed keywords


EID: 84926307250     PISSN: 17564646     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jff.2015.01.043     Document Type: Review
Times cited : (386)

References (52)
  • 1
    • 0042788736 scopus 로고    scopus 로고
    • Determination of the hydrophilic and lipophilic antioxidant activity of white and red wines during the wine-making process
    • Alcolea J.F., Cano A., Acosta M., Arnao M.B. Determination of the hydrophilic and lipophilic antioxidant activity of white and red wines during the wine-making process. Italian Journal of Food Science 2003, 15:207-214.
    • (2003) Italian Journal of Food Science , vol.15 , pp. 207-214
    • Alcolea, J.F.1    Cano, A.2    Acosta, M.3    Arnao, M.B.4
  • 4
    • 0035057526 scopus 로고    scopus 로고
    • The hydrophilic and lipophilic contribution to total antioxidant activity
    • Arnao M.B., Cano A., Acosta M. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry 2001, 73:239-244.
    • (2001) Food Chemistry , vol.73 , pp. 239-244
    • Arnao, M.B.1    Cano, A.2    Acosta, M.3
  • 8
    • 0031232088 scopus 로고    scopus 로고
    • Kinetics and mechanisms of antioxidant activity using the DPPH free radical method
    • Bondet V., Brand-Williams W., Berset C. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. LWT - Food Science and Technology 1997, 30:609-615.
    • (1997) LWT - Food Science and Technology , vol.30 , pp. 609-615
    • Bondet, V.1    Brand-Williams, W.2    Berset, C.3
  • 14
    • 1842505047 scopus 로고    scopus 로고
    • Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH radical in alcoholic solutions
    • Foti M.C., Daquino C., Geraci C. Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH radical in alcoholic solutions. The Journal of Organic Chemistry 2004, 69:2309-2314.
    • (2004) The Journal of Organic Chemistry , vol.69 , pp. 2309-2314
    • Foti, M.C.1    Daquino, C.2    Geraci, C.3
  • 15
    • 57449110347 scopus 로고    scopus 로고
    • Reaction of phenols with the 2,2-diphenyl-1-picrylhydrazyl radical. Kinetics and DFT calculations applied to determine ArO-H bond dissociation enthalpies and reaction mechanism
    • Foti M.C., Daquino C., Mackie I.D., DiLabio G.A., Ingold K.U. Reaction of phenols with the 2,2-diphenyl-1-picrylhydrazyl radical. Kinetics and DFT calculations applied to determine ArO-H bond dissociation enthalpies and reaction mechanism. The Journal of Organic Chemistry 2008, 73:9270-9282.
    • (2008) The Journal of Organic Chemistry , vol.73 , pp. 9270-9282
    • Foti, M.C.1    Daquino, C.2    Mackie, I.D.3    DiLabio, G.A.4    Ingold, K.U.5
  • 17
    • 47849087389 scopus 로고    scopus 로고
    • How to standardize the multiplicity of methods to evaluate natural antioxidants
    • Frankel E.N., Finley J. How to standardize the multiplicity of methods to evaluate natural antioxidants. Journal of Agricultural and Food Chemistry 2008, 56:4901-4908.
    • (2008) Journal of Agricultural and Food Chemistry , vol.56 , pp. 4901-4908
    • Frankel, E.N.1    Finley, J.2
  • 18
  • 20
    • 0025035276 scopus 로고
    • Phycoerythrin fluorescence-based assay for reactive oxygen species
    • Glazer A.N. Phycoerythrin fluorescence-based assay for reactive oxygen species. Methods in Enzymology 1990, 186:161-168.
    • (1990) Methods in Enzymology , vol.186 , pp. 161-168
    • Glazer, A.N.1
  • 21
    • 13844298231 scopus 로고    scopus 로고
    • Antioxidant or not. Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: Direct or indirect effects?
    • Halliwell B., Rafter J., Jenner A. Antioxidant or not. Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: Direct or indirect effects?. The American Journal of Clinical Nutrition 2005, 81:268S-276S.
    • (2005) The American Journal of Clinical Nutrition , vol.81 , pp. 268S-276S
    • Halliwell, B.1    Rafter, J.2    Jenner, A.3
  • 23
    • 0001391603 scopus 로고
    • The kinetics of reaction of 2,2-diphenyl-1-picrylhydrazyl with phenols
    • Hogg J.S., Lohmann D.H., Russell K.S. The kinetics of reaction of 2,2-diphenyl-1-picrylhydrazyl with phenols. Canadian Journal of Chemistry 1961, 39:1588-1594.
    • (1961) Canadian Journal of Chemistry , vol.39 , pp. 1588-1594
    • Hogg, J.S.1    Lohmann, D.H.2    Russell, K.S.3
  • 24
    • 0037181273 scopus 로고    scopus 로고
    • Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated b-cyclodextrin as the solubility enhancer
    • Huang D., Ou B., Hampsch-Woodill M., Flanagan J.A., Deemer E.K. Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated b-cyclodextrin as the solubility enhancer. Journal of Agricultural and Food Chemistry 2002, 50:1815-1821.
    • (2002) Journal of Agricultural and Food Chemistry , vol.50 , pp. 1815-1821
    • Huang, D.1    Ou, B.2    Hampsch-Woodill, M.3    Flanagan, J.A.4    Deemer, E.K.5
  • 25
    • 0037205604 scopus 로고    scopus 로고
    • High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format
    • Huang D., Ou B., Hampsch-Woodill M., Flanagan J.A., Prior R.L. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. Journal of Agricultural and Food Chemistry 2002, 50:4437-4444.
    • (2002) Journal of Agricultural and Food Chemistry , vol.50 , pp. 4437-4444
    • Huang, D.1    Ou, B.2    Hampsch-Woodill, M.3    Flanagan, J.A.4    Prior, R.L.5
  • 30
    • 0027509770 scopus 로고
    • A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates
    • Miller N.J., Rice-Evans C.A., Davies M.J., Gopinathan V., Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science 1993, 84:407-412.
    • (1993) Clinical Science , vol.84 , pp. 407-412
    • Miller, N.J.1    Rice-Evans, C.A.2    Davies, M.J.3    Gopinathan, V.4    Milner, A.5
  • 31
    • 0037422460 scopus 로고    scopus 로고
    • Quantitative structure toxicity relationships for phenols in isolated rat hepatocytes
    • Moridani M.Y., Siraki A., O'Brien P.J. Quantitative structure toxicity relationships for phenols in isolated rat hepatocytes. Chemico-Biological Interactions 2003, 145:213-223.
    • (2003) Chemico-Biological Interactions , vol.145 , pp. 213-223
    • Moridani, M.Y.1    Siraki, A.2    O'Brien, P.J.3
  • 34
    • 0034773950 scopus 로고    scopus 로고
    • Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe
    • Ou B., Hampsch-Woodill M., Prior R.L. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. Journal of Agricultural and Food Chemistry 2001, 49:4619-4626.
    • (2001) Journal of Agricultural and Food Chemistry , vol.49 , pp. 4619-4626
    • Ou, B.1    Hampsch-Woodill, M.2    Prior, R.L.3
  • 36
    • 20844445334 scopus 로고    scopus 로고
    • Standard methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements
    • Prior R.L., Wu X., Schaich K.M. Standard methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry 2005, 53:4290-4302.
    • (2005) Journal of Agricultural and Food Chemistry , vol.53 , pp. 4290-4302
    • Prior, R.L.1    Wu, X.2    Schaich, K.M.3
  • 38
    • 79961046338 scopus 로고    scopus 로고
    • Hydrogen atom abstraction reactions from tertiary amines by benzyloxyl and cumyloxyl radicals: Influence of structure on the rate-determining formation of a hydrogen-bonded prereaction complex
    • Salamone M., DiLabio G.A., Bietti M. Hydrogen atom abstraction reactions from tertiary amines by benzyloxyl and cumyloxyl radicals: Influence of structure on the rate-determining formation of a hydrogen-bonded prereaction complex. The Journal of Organic Chemistry 2011, 76:6264-6270.
    • (2011) The Journal of Organic Chemistry , vol.76 , pp. 6264-6270
    • Salamone, M.1    DiLabio, G.A.2    Bietti, M.3
  • 39
    • 84867386015 scopus 로고    scopus 로고
    • Hydrogen abstraction from cyclic amines by the cumyloxyl and benzyloxyl radicals. The role of stereoelectronic effects and of substrate/radical hydrogen bonding
    • Salamone M., Martella R., Bietti M. Hydrogen abstraction from cyclic amines by the cumyloxyl and benzyloxyl radicals. The role of stereoelectronic effects and of substrate/radical hydrogen bonding. The Journal of Organic Chemistry 2012, 77:8556-8561.
    • (2012) The Journal of Organic Chemistry , vol.77 , pp. 8556-8561
    • Salamone, M.1    Martella, R.2    Bietti, M.3
  • 40
    • 0018147491 scopus 로고
    • Fluorescence properties of free and protein bound fluorescein dyes. I. Macrospectrofluorometric measurements
    • Schauenstein K., Schauenstein E., Wick G. Fluorescence properties of free and protein bound fluorescein dyes. I. Macrospectrofluorometric measurements. The Journal of Histochemistry and Cytochemistry 1978, 26:277-283.
    • (1978) The Journal of Histochemistry and Cytochemistry , vol.26 , pp. 277-283
    • Schauenstein, K.1    Schauenstein, E.2    Wick, G.3
  • 42
    • 33244495293 scopus 로고    scopus 로고
    • Determination of total content of phenolic compounds and their antioxidant activity in vegetables - Evaluation of spectrophotometric methods
    • Stratil P., Klejdus B., Kuban V. Determination of total content of phenolic compounds and their antioxidant activity in vegetables - Evaluation of spectrophotometric methods. Journal of Agricultural and Food Chemistry 2006, 54:607-616.
    • (2006) Journal of Agricultural and Food Chemistry , vol.54 , pp. 607-616
    • Stratil, P.1    Klejdus, B.2    Kuban, V.3
  • 44
    • 84879005169 scopus 로고    scopus 로고
    • Effects of molecular structure on kinetics and dynamics of the Trolox equivalent antioxidant capacity assay with ABTS
    • Tian X., Schaich K.M. Effects of molecular structure on kinetics and dynamics of the Trolox equivalent antioxidant capacity assay with ABTS. Journal of Agricultural and Food Chemistry 2013, 61:5511-5519.
    • (2013) Journal of Agricultural and Food Chemistry , vol.61 , pp. 5511-5519
    • Tian, X.1    Schaich, K.M.2
  • 46
    • 0032987218 scopus 로고    scopus 로고
    • Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures
    • van den Berg R., Haenen G.R.M.M., van den Berg H., Bast A. Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chemistry 1999, 66:511-517.
    • (1999) Food Chemistry , vol.66 , pp. 511-517
    • van den Berg, R.1    Haenen, G.R.M.M.2    van den Berg, H.3    Bast, A.4
  • 47
    • 12244296932 scopus 로고
    • Structure of 2,2-diphenyl-1-picrylhydrazyl free radical
    • Williams D.E. Structure of 2,2-diphenyl-1-picrylhydrazyl free radical. Journal of the American Chemical Society 1966, 88:5665-5666.
    • (1966) Journal of the American Chemical Society , vol.88 , pp. 5665-5666
    • Williams, D.E.1
  • 48
    • 64549150986 scopus 로고    scopus 로고
    • Effect of drying methods with the application of vacuum microwaves on the bioactive compounds, colour, and antioxidant activity of strawberry fruits
    • Wojdyło A., Figiel A., Oszmiański J. Effect of drying methods with the application of vacuum microwaves on the bioactive compounds, colour, and antioxidant activity of strawberry fruits. Journal of Agricultural and Food Chemistry 2009, 57:1337-1343.
    • (2009) Journal of Agricultural and Food Chemistry , vol.57 , pp. 1337-1343
    • Wojdyło, A.1    Figiel, A.2    Oszmiański, J.3
  • 49
    • 84899836374 scopus 로고    scopus 로고
    • Provided by R. Prior, Univ. Arkansas
    • FL) 2005, Provided by R. Prior, Univ. Arkansas.
    • (2005) FL)
    • Wu, X.1
  • 51
    • 84900802785 scopus 로고    scopus 로고
    • Re-evaluation of the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity
    • Xie J., Schaich K.M. Re-evaluation of the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity. Journal of Agricultural and Food Chemistry 2014, 62:4251-4260.
    • (2014) Journal of Agricultural and Food Chemistry , vol.62 , pp. 4251-4260
    • Xie, J.1    Schaich, K.M.2
  • 52
    • 0036876103 scopus 로고    scopus 로고
    • Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid
    • Yen G.-C., Duh P.-D., Tsai H.-L. Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chemistry 2002, 79:307-313.
    • (2002) Food Chemistry , vol.79 , pp. 307-313
    • Yen, G.-C.1    Duh, P.-D.2    Tsai, H.-L.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.