Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe

Journal of Agricultural and Food Chemistry

Volumn 49, Issue 10, 2001, Pages 4619-4626

  Ou, B ^a   Hampsch Woodill, M ^a   Prior, R L ^b  

^a Brunswick Laboratories   (United States)

^b U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

Chain breaking antioxidant; Fluorescein; FRAP; Free radical; Hydrogen atom transfer; ORAC; Single electron transfer; TEAC

Indexed keywords

6-HYDROXY-2,5,7,8-TETRAMETHYLCHROMAN-2-CARBOXYLIC ACID; ANTIOXIDANT; CHROMAN DERIVATIVE; FLUORESCEIN; FLUORESCENT DYE; OXYGEN RADICAL; PERHYDROXYL RADICAL; PEROXIDE; PHYCOERYTHRIN; REACTIVE OXYGEN METABOLITE; TROLOX C;

ABSORPTION; ANALYTIC METHOD; ANTIOXIDANT ACTIVITY; ARTICLE; CHEMISTRY; ELECTRON TRANSPORT; FOOD ANALYSIS; FRUIT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHILICITY; INTERMETHOD COMPARISON; MASS SPECTROMETRY; METHODOLOGY; OXIDATION REDUCTION REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TEA; VALIDATION PROCESS;

ANTIOXIDANTS; CHROMANS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLUORESCEIN; FLUORESCENT DYES; FOOD ANALYSIS; FRUIT; MASS SPECTROMETRY; OXIDATION-REDUCTION; PEROXIDES; PHYCOERYTHRIN; REACTIVE OXYGEN SPECIES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TEA;

EID: 0034773950     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf010586o     Document Type: Article

References (27)

1. DNA damage by oxygen derived species. Its mechanism and measurement in mammalian systems
2. DNA deaminating ability and genotoxicity of nitric oxide and its progenitors
3. DNA damage and mutation in human cells exposed to nitric oxide in vitro
4. Protein modification in oxidative stress
5. Oxygen-radical absorbance capacity assay for antioxidants
6. Automated assay of oxygen radical absorbance capacity with the COBAS FARA II
7. The measurement of oxygen radical absorbance capacity in biological samples
8. Fluorescence-based assay for reactive oxygen species: A protective role for creatinine
9. Phycoerythrin fluorescence-based assay for reactive oxygen species
10. Polyphenol-protein interactions
11. The association of proteins with polyphenols
12. Structure features of procyanidin interactions with salivary protein
13. 1H NMR spectroscopy
14. Nature of polyphenol-protein ineractions
15. Detection of DNA replication intermediates after two-dimensional agarose gel electrophoresis using a fluorescein-labeled probe
16. Rational design of fluorescein-based fluorescence probes. Mechanism-based design of a maximum fluorescence probe for singlet oxygen
17. Fluorescent indicators for imaging nitric oxide production
18. Theoretical calculation of substituent effects on the O-H bond strength of phenolic antioxidants related to vitamin E
19. On the antioxidant mechanism of curcumin: Classic methods are needed to determine antioxidant mechanism and activity
20. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay
21. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates
22. Comparison of the analytical methods for assessing the total antioxidant capacity of human serum
23. Measurement of total antioxidant activity as a marker of antioxidant status in vivo: Procedures and limitations
24. Radical cation as reference chromogens in kinetic studies of one-electron-transfer reaction: Plus radiolysis studies of 2,2′-azinobis-(3-ethylbenzthiaoline-6-sulphonate)
25. Antioxidant activity applying an improved ABTS radical cation decolorization assay
26. Spectroscopic parameters, electrode potentials, acid ionization constants, and electron exchange rates of the 2,2′-Azinobis(3-ethylbenzothiazoline-6-sulfonate) radicals and ions
27. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay