Nutraceutical properties of phycocyanin

Journal of Functional Foods

Volumn 11, Issue C, 2014, Pages 375-392

  Fernández Rojas, Berenice ^a   Hernández Juárez, Jesús ^b   Pedraza Chaverri, José ^a  

^a UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^b INSTITUTO MEXICANO DEL SEGURO SOCIAL   (Mexico)

Author keywords

Functional food; Nutraceutical; Phycobyliprotein; Phycocyanin; Scavenging activity

Indexed keywords

ALGAE; SPIRULINA;

EID: 84926283713     PISSN: 17564646     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jff.2014.10.011     Document Type: Review

References (140)

1. Abalde J. Purification and characterization of phycocyanin from the marine cyanobacterium Synechococcus sp. 109201. Plant Science 1998, 136:109-120.
2. Abeliovich A., Shilo M. Photooxidative reactions of C-phycocyanin. Biochimica et Biophysica Acta 1972, 2:483-491.
3. Ahmadi A., Gharipour M., Arabzadeh G., Moin P., Hashemipour M., Kelishadi R. The effects of vitamin E and omega-3 PUFAs on endothelial function among adolescents with metabolic syndrome. BioMed Research International 2014, 2014:906019. 10.1155/2014/906019.
4. Ahsan M.B., Mashuda H.P., Tim C.H., Mohammad R.H. A review on culture, production and use of Spirulina as food for humans and feeds for domestic animals and fish (Vol. 1034, pp. 1-25) 2008, FAO Fisheries and Aquaculture Circular No. 1034, Rome, Italy.
5. Antelo F.S., Anschau A., Costa J.A., Kalil S.J. Extraction and Purification of C-phycocyanin from. Journal of the Brazilian Chemical Society 2010, 21(5):921-926.
6. Avila-Nava A., Calderón-Oliver M., Medina-Campos O.N., Zou T., Gu L., Torres N., Tovar A., Pedraza-Chaverri J. Extract of cactus (Opuntia ficus indica) cladodes scavenges reactive oxygen species in vitro and enhances plasma antioxidant capacity in humans. Journal of Functional Foods 2014, 10:13-24.
7. Benedetti S., Benvenuti F., Pagliarani S., Francogli S., Scoglio S., Canestrari F. Antioxidant properties of a novel phycocyanin extract from the blue-green alga Aphanizomenon flos-aquae. Life Sciences 2004, 75:2353-2362.
8. Benedetti S., Benvenuti F., Scoglio S., Canestrari F. Oxygen radical absorbance capacity of phycocyanin and phycocyanobilin from the food supplement Aphanizomenon flos-aquae. Journal of Medicinal Food 2010, 13(1):223-227.
9. Benedetti S., Rinalducci S., Benvenuti F., Francogli S., Pagliarani S., Giorgi L., Micheloni A., D'Amici G.M., Zolla L., Canestrari F. Purification and characterization of phycocyanin from the blue-green alga Aphanizomenon flos-aquae. Journal of Chromatography B 2006, 833:12-18.
10. Bennett A., Bogorad L. Complementary chromatic adaptation in a filamentous blue-green alga. The Journal of Cell Biology 1973, 58:419-435.
11. Bermejo P., Pinero E., Villar A. Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis. Food Chemistry 2008, 110:436-445.
12. Bhat V.B., Madyastha K.M. Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: Protection against oxidative damage to DNA. Biochemical and Biophysical Research Communications 2001, 285(2):226-262.
13. Blanco-Ayala T., Andérica-Romero A.C., Pedraza-Chaverri J. New insights into antioxidant strategies against paraquat toxicity. Free Radical Research 2014, 48(6):623-640.
14. Braithwaite M.C., Tyagi C., Tomar L.K., Kumar P., Choonara Y.E., Pillay V. Nutraceutical-based therapeutics and formulation strategies augmenting their efficiency to complement modern medicine: An overview. Journal of Functional Foods 2014, 6:82-99.
15. Chaiklahan R., Chirasuwan N., Loha V., Tia S., Bunnag B. Separation and purification of phycocyanin from Spirulina sp. using a membrane process. Bioresource Technology 2011, 102(14):7159-7164.
16. Chapman D.J., Cole W.J., Siegelman H.W. Phylogenetic implications of phycocyanobilin and C-phycocyanin. American Journal of Botany 1968, 55(3):314-316.
17. Chattopadhyay P.K., Gaylord B., Palmer A., Jiang N., Raven M.A., Lewis G., Reuter M., Nur-ur A.K.M., Price D., Roederer M. Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments. Cytometry. Part A: The Journal of the International Society for Analytical Cytology 2012, 81(6):456-466.
18. Chen J.-C., Liu K.S., Yang T.-J., Hwang J.-H., Chan Y.-C., Lee I.-T. Spirulina and C-phycocyanin reduce cytotoxicity and inflammation-related genes expression of microglial cells. Nutritional Neuroscience 2012, 15(6):252-256.
19. Chen T., Wong Y.-S. In vitro antioxidant and antiproliferative activities of selenium-containing phycocyanin from selenium-enriched Spirulina platensis. Journal of Agricultural and Food Chemistry 2008, 56(12):4352-4358.
20. Cherng S.-C., Cheng S.-N., Tarn A., Chou T.-C. Anti-inflammatory activity of c-phycocyanin in lipopolysaccharide-stimulated RAW 264.7 macrophages. Life Sciences 2007, 81:1431-1435.
21. Chu W.-L., Lim Y.-W., Radhakrishnan A.K., Lim P.-E. Protective effect of aqueous extract from Spirulina platensis against cell death induced by free radicals. BMC Complementary and Alternative Medicine 2010, 10(53). 10.1186/1472-6882-10-53.
22. Chuner C., Lian W., Chunxia L., Peimin H., Jie L., Jiahai Z. Purification, crystallization communications purification, crystallization and preliminary X-ray analysis of phycocyanin and phycoerythrin from Porphyra yezoensis Ueda. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 2011, F67:579-583.
23. Custódio J.B.A., Cardoso C.M.P., Santos M.S., Almeida L.M., Vicente J.A.F., Fernandes M.A.S. Cisplatin impairs rat liver mitochondrial functions by inducing changes on membrane ion permeability: Prevention by thiol group protecting agents. Toxicology 2009, 259(1-2):18-24.
24. De Jesus Raposo M.F., de Morais R.M.S.C., de Morais A.M.M.B. Health applications of bioactive compounds from marine microalgae. Life Sciences 2013, 93(15):479-486.
25. De Marsac N.T., Cohen-Bazire G. Molecular composition of cyanobacterial phycobilisomes. Proceedings of the National Academy of Sciences of the United States of America 1977, 74(4):1635-1639.
26. Durackova Z. Some current insights into oxidative stress. Physiological Research 2010, 59(4):459-469.
27. El-Ayouty, Y., Baredy, M., & Salama, A. M. (2007). Effect of phycocyanin pigment on acute renal failure induced by mercuric chloride. Proceeding of the second scientific environmental conference, Zagazig UNI. (pp. 87-95).
28. Eriksen N.T. Production of phycocyanin - A pigment with applications in biology, biotechnology, foods and medicine. Applied Microbiology and Biotechnology 2008, 80(1):1-14.
29. Fabbrocini G., Cameli N., Lorenzi S., De Padova M.P., Marasca C., Izzo R., Monfrecola G. A dietary supplement to reduce side effects of oral isotretinoin therapy in acne patients. Giornale Italiano di Dermatologia e Venereologia 2014, 149(4):441-445.
30. Farooq S., Asokan D., Sakthivel R., Kalaiselvi P., Varalakshmi P. Salubrious effect of C-phycocyanin against oxalate-mediated renal cell injury. Clinica Chimica Acta 2004, 348(1-2):199-205.
31. Farooq S.M., Boppana N.B., Asokan D., Sekaran S.D., Shankar E.M., Li C., Gopal C., Bakar S., Karthik H., Ebrahim A.S. C-phycocyanin confers protection against oxalate-mediated oxidative stress and mitochondrial dysfunctions in MDCK cells. PLoS ONE 2014, 9(4):e93056. 10.1371/journal.pone.0093056.
32. Farooq S.M., Ebrahim A.S., Subramhanya K.H., Sakthivel R., Rajesh N.G., Varalakshmi P. Oxalate mediated nephronal impairment and its inhibition by c-phycocyanin: A study on urolithic rats. Molecular and Cellular Biochemistry 2006, 284:95-101.
33. FDA Food Agency Response Letter GRAS Notice No. GRN 000424 Accessed 12.08.14. http://www.fda.gov/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/ucm335743.htm.
34. Fergurson L. Nutrigenomics approaches to functional foods. Journal of the American Dietetic Association 2009, 109:452-458.
35. Fernandez-Rojas B., Medina-Campos O.N., Hernandez-Pando R., Negrette-Guzman M., Huerta-Yepez S., Pedraza-Chaverri J. C-phycocyanin prevents cisplatin-induced nephrotoxicity through inhibition of oxidative stress. Food & Function 2014, 5:480-490.
36. Gantar M., Simović D., Djilas S., Gonzalez W.W., Miksovska J. Isolation, characterization and antioxidative activity of C-phycocyanin from Limnothrix sp. strain 37-2-1. Journal of Biotechnology 2012, 159:21-26.
37. Glazer A.A.N., Cohen-Bazire G., Jul N. Subunit structure of the phycobiliproteins of blue-green algae subunit structure of the phycobiliproteins of blue-green algae. Proceedings of the National Academy of Sciences of the United States of America 1971, 68(7):1398-1401.
38. Glazer A.N., Stryer L. Fluorescent tandem phycobiliprotein conjugates. Biophysical Journal 1983, 43:383-386.
39. Gonzalez R., Gonzalez A., Remirez D., Romay C., Rodriguez S., Ancheta O., Merino N. Protective effects of phycocyanin on galactosamine-induced hepatitis in rats. Biotecnología Aplicada 2003, 20:107-110.
40. Griffiths J.C., Abernethy D.R., Schuber S., Williams R.L. Functional food ingredient quality: Opportunities to improve public health by compendia standardization. Journal of Functional Foods 2009, 1:128-130.
41. Gupta M., Dwivedi U.N., Khandelwal S. C-phycocyanin: An effective protective agent against thymic atrophy by tributyltin. Toxicology Letters 2011, 204(1):2-11.
42. Halliwell B. Antioxidants in human health and disease. Annual Review of Nutrition 1996, 16:33-50.
43. Hsiao G., Chou P.-H., Shen M.-Y., Chou D.-S., Lin C.-H., Sheu J.-R. C-phycocyanin, a very potent and novel platelet aggregation inhibitor from Spirulina platensis. Journal of Agricultural and Food Chemistry 2005, 53(20):7734-7740.
44. Huang Z., Guo B.J., Wong R.N.S., Jiang Y. Characterization and antioxidant activity of selenium-containing phycocyanin isolated from Spirulina platensis. Food Chemistry 2007, 100(3):1137-1143.
45. Ivanova K.G., Stankova K.G., Nikolov V.N., Georgieva R.T., Minkova K.M., Gigova L.G., Rupova I., Boteva R.N. The biliprotein C-phycocyanin modulates the early radiation response: A pilot study. Mutation Research 2010, 695:40-45.
46. Kaplan M., Mutlu E.A., Benson M., Fields J.Z., Banan A., Keshavarzian A. Use of herbal preparations in the treatment of oxidant-mediated inflammatory disorders. Complementary Therapies in Medicine 2007, 15(3):207-216.
47. Khan M., Varadharaj S., Ganesan L.P., Shobha J.C., Naidu M.U., Parinandi N.L., Tridandapani S., Kumar V., Kuppusamy P. C-phycocyanin protects against ischemia-reperfusion injury of heart through involvement of p38 MAPK and ERK signaling. American Journal of Physiology, Heart and Circulatory Physiology 2006, 290(5):H2136-H2145.
48. Khan M., Varadharaj S., Shobha J.C., Naidu M., Parinandi N., Kumar V., Kuppusamy P. C-phycocyanin ameliorates doxorubicin-induced oxidative stress and apoptosis in adult rat cardiomyocytes. Journal of Cardiovascular Pharmacology 2006, 47(1):9-20.
49. Kim S.-K., Ravichandran Y.D., Khan S.B., Kim Y.T. Prospective of the cosmeceuticals derived from marine organisms. Biotechnology and Bioprocess Engineering 2008, 13(5):511-523.
50. Klaassen C. Casarett and Doull's toxicology: The basic science of poisons 2007, McGraw-Hill Medica, New York. 7th ed.
51. Kothadia A., Shenoy A., Shabaraya A., Rajan M., Viradia U., Patel N. Evaluation of cataract preventive action of phycocyanin. International Journal of Pharmaceutical Sciences and Drug Research 2011, 3(1):42-44.
52. Kuddus M., Singh P., Thomas G., Al-Hazimi A. Recent developments in production and biotechnological applications of C-phycocyanin. BioMed Research International 2013, 2013:742859. 10.1155/2013/742859.
53. Kumar D., Gaur J.P. Growth and metal removal potential of a Phormidium bigranulatum-dominated mat following long-term exposure to elevated levels of copper. Environmental Science and Pollution Research International 2014, 21(17):10279-10285.
54. Kumari R.P., Anbarasu K. Protective role of C-phycocyanin against secondary changes during sodium selenite mediated cataractogenesis. Natural Products and Bioprospecting 2014, 4:81-89.
55. Lakshmi P., Maheswari U., Annamalai A. Sequence and structure comparison studies of phycocyanin in Spirulina Platensis Journal of Computer Science & Systems Biology. Journal of Computer Science & Systems Biology 2008, 1:63-72.
56. Lattanzio V., Kroon P.A., Linsalata V., Cardinali A. Globe artichoke: A functional food and source of nutraceutical ingredients. Journal of Functional Foods 2009, 1:131-144.
57. Leung P.-O., Lee H.-H., Kung Y.-C., Tsai M.-F., Chou T.-C. Therapeutic effect of C-phycocyanin extracted from blue green algae in a rat model of acute lung injury induced by lipopolysaccharide. Evidence-based Complementary and Alternative Medicine 2013, 2013, 916590.
58. Li B., Chu X., Gao M., Li W. Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin. Acta Biochimica et Biophys Sinica 2010, 42(1):80-89.
59. Li X., Chen T., Wong Y., Xu G., Fan R. Involvement of mitochondrial dysfunction in human islet amyloid polypeptide-induced apoptosis in INS-1E pancreatic beta cells: An effect attenuated by phycocyanin. International Journal of Biochemistry and Cell Biology 2011, 43(4):525-534.
60. Liron D., Prado M., Arteni A.A., Elmlund D.A., Blankenship R.E., Adir N. Structural studies show energy transfer within stabilized phycobilisomes independent of the mode of rod-core assembly. Biochimica et Biophysica Acta 2014, 1837(3):385-395.
61. Lissi E., Pizarro M., Aspee A., Romay C. Kinetics of phycocyanine bilin groups destruction by peroxyl radicals. Free Radical Biology & Medicine 2000, 28(7):1051-1055.
62. Lobo V., Patil A., Phatak A., Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews 2010, 4(8):118-126.
63. MacColl R. Allophycocyanin and energy transfer. Biochimica et Biophysica Acta 2004, 1657(2-3):73-81.
64. Madhyastha H.K., Sivashankari S., Vatsala T.M. C-phycocyanin from Spirulina fussiformis exposed to blue light demonstrates higher efficacy of in vitro antioxidant activity. Biochemical Engineering Journal 2009, 43(2):221-224.
65. Madhyastha H., Vatsala T.M. Cysteine-rich cyanopeptide beta2 from Spirulina fusiformis exhibits plasmid DNA pBR322 scission prevention and cellular antioxidant activity. Indian Journal of Experimental Biology 2010, 48(5):486-493.
66. Manconia M., Pendas J., Ledón N., Moreira T., Sinico C., Saso L., Fadda A.M. Phycocyanin liposomes for topical anti-inflammatory activity: In-vitro in-vivo studies. The Journal of Pharmacy and Pharmacology 2009, 61:423-430.
67. Marín-Prida J., Pavón-Fuentes N., Llópiz-Arzuaga A., Fernandez-Massó J.R., Delgado-Roche L., Mendoza-Marí Y., Pedroso S., Cruz-Ramírez A., Valenzuela-Silva C., Nazabal-Galvez M., Cintado-Benítez A., Pardo-Andreu G., Polentarutti N., Riva F., Pentón-Arias E., Pentón-Rol G. Phycocyanobilin promotes PC12 cell survival and modulates immune and inflammatory genes and oxidative stress markers in acute cerebral hypoperfusion in rats. Toxicology and Applied Pharmacology 2013, 272:49-60.
68. 2+/phosphate-induced impairment. Brain Research Bulletin 2012, 89:159-167.
69. McCarty M.F. Clinical potential of phycocyanobilin for induction of T regulatory cells in the management of inflammatory disorders. Medical Hypotheses 2011, 77(6):1031-1033.
70. Menezes A.R., Lamb M.C., Lavie C.J., DiNicolantonio J.J. Vitamin D and atherosclerosis. Current Opinion in Cardiology 2014, 29. 10.1097/HCO.0000000000000108.
71. Minkova K.M., Tchernov A.A., Tchorbadjieva M.I., Fournadjieva S.T., Antova R.E., Busheva M.C. Purification of C-phycocyanin from Spirulina (Arthrospira) fusiformis. Journal of Biotechnology 2003, 102(1):55-59.
72. Minkova K., Tchorbadjieva M., Tchernov A., Stojanova M., Gigova L., Busheva M. Improved procedure for separation and purification of Arthronema africanum phycobiliproteins. Biotechnology Letters 2007, 29(4):647-651.
73. Morcos N.C., Berns M., Henry W. Phycocyanin: Laser activation, cytotoxic effects, and uptake in human atherosclerotic plaque. Laser in Surgery and Medicine 1988, 8(1):10-17.
74. Neerati P., Devde R., Gangi A.K. Evaluation of the effect of curcumin capsules on glyburide therapy in patients with type-2 diabetes mellitus. Phytotherapy Research 2014, 10.1002/ptr.5201.
75. Nishanth R.P., Ramakrishna B.S., Jyotsna R.G., Roy K.R., Reddy G., Reddy P.K., Reddanna P. C-phycocyanin inhibits MDR1 through reactive oxygen species and cyclooxygenase-2 mediated pathways in human hepatocellular carcinoma cell line. European Journal of Pharmacology 2010, 649:74-83.
76. Niu J.-F., Wang G.-C., Lin X., Zhou B.-C. Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 2007, 850(1-2):267-276.
77. Oliveira E.G., Rosa G.S., Moraes M.A., Pinto L.A.A. Characterization of thin layer drying of Spirulina platensis utilizing perpendicular air flow. Bioresource Technology 2009, 100(3):1297-1303.
78. Ou Y., Lin L., Pan Q., Yang X., Cheng X. Preventive effect of phycocyanin from Spirulina platensis on alloxan-injured mice. Environmental Toxicology and Pharmacology 2012, 34(3):721-726.
79. Ou Y., Zheng S., Lin L., Jiang Q., Yang X. Protective effect of C-phycocyanin against carbon tetrachloride-induced hepatocyte damage in vitro and in vivo. Chemico-Biological Interactions 2010, 185(2):94-100.
80. Padyana A.K., Bhat V.B., Madyastha K.M., Rajashankar K.R., Ramakumar S. Crystal structure of a light-harvesting protein C-phycocyanin from Spirulina platensis. Biochemical and Biophysical Research Communications 2001, 282(4):893-898.
81. Pangestuti R., Kim S.-K. Biological activities and health benefit effects of natural pigments derived from marine algae. Journal of Functional Foods 2011, 3(4):255-266.
82. Patel A., Mishra S., Pawar R., Ghosh P.K. Purification and characterization of C-phycocyanin from cyanobacterial species of marine and freshwater habitat. Protein Expression and Purification 2005, 40:248-255.
83. Patel A., Pawar R., Mishra S., Sonawane S., Ghosh P.K. Kinetic studies on thermal denaturation of C-phycocyanin. Indian Journal of Biochemistry & Biophysics 2004, 41:254-257.
84. Patel A., Sandhya M., Ghosh P.K. Antioxidant potential of C-phycocyanin isolated from cyanobacterial species Lyngbya, Phormidium and Spirulina spp. Indian Journal of Biochemistry & Biophysics 2006, 43:25-31.
85. Patil G., Chethana S., Madhusudhan M.C., Raghavarao K.S.M.S. Fractionation and purification of the phycobiliproteins from Spirulina platensis. Bioresource Technology 2008, 99(15):7393-7396.
86. Patil G., Chethana S., Sridevi A.S., Raghavarao K.S.M.S. Method to obtain C-phycocyanin of high purity. Journal of Chromatography. A 2006, 1127(1-2):76-81.
87. Wang X.Q., Li L.N., Chang W.R., Zhang J.P., Gui L.L., Guo B.J., Liang D.C. Structure of C-phycocyanin from Spirulina platensis at 2.2 A resolution: A novel monoclinic crystal form for phycobiliproteins in phycobilisomes. Acta Crystallographica. Section D, Biological Crystallography 2001, 57:784-792. PDB ID 1GH0.
88. Pentón-Rol G., Marín-Prida J., Pardo-Andreu G., Martínez-Sanchez G., Acosta-Medina E.F., Valdivia-Acosta A., Lagumersindez-Denis N., Rodríguez-Jiménez E., Llópiz-Arzuaga A., López-Saura P., Guillén-Nieto G., Pentón-Arias E. C-phycocyanin is neuroprotective against global cerebral ischemia/reperfusion injury in gerbils. Brain Research Bulletin 2011, 86(1-2):42-52.
89. Pentón-Rol G., Martínez-Sanchez G., Cervantes-Llanos M., Lagumersindez-Denis N., Acosta-Medina E.F., Falcón-Cama V., Alonso-Ramírez R., Valenzuela-Silva C., Rodríguez-Jiménez E., Llópiz-Arzuaga A., Marín-Prida J., López-Saura P.A., Guillén-Nieto G.E., Pentón-Arias E. C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells. International Immunopharmacology 2011, 11:29-38.
90. Pleonsil P., Soogarun S., Suwanwong Y. Anti-oxidant activity of holo- and apo-c-phycocyanin and their protective effects on human erythrocytes. International Journal of Biological Macromolecules 2013, 60:393-398.
91. Rimbau V., Camins A., Pubill D., Sureda F., Romay C., Gonzalez R., Jiménez A., Escubedo E., Camarasa J., Pallàs M. C-phycocyanin protects cerebellar granule cells from low potassium/serum deprivation-induced apoptosis. Naunyn-Schmiedeberg's Archives of Pharmacology 2001, 364:96-104. 10.1007/s002100100437.
92. Rimbau V., Camins A., Romay C., Gonzalez R., Pallàs M. Protective effects of C-phycocyanin against kainic acid-induced neuronal damage in rat hippocampus. Neuroscience Letters 1999, 276(2):75-78.
93. Rinalducci S., Roepstorff P., Zolla L. De novo sequence analysis and intact mass measurements for characterization of phycocyanin subunit isoforms from the blue-green alga Aphanizomenon flos-aquae. Journal of Mass Spectrometry 2009, 44:503-515.
94. Riss J., Décordé K., Sutra T., Delage M., Baccou J.-C., Jouy N., Brune J.P., Oréal H, Cristol J.P., Rouanet J.-M. Phycobiliprotein C-phycocyanin from Spirulina platensis is powerfully responsible for reducing oxidative stress and NADPH oxidase expression induced by an atherogenic diet in hamsters. Journal of Agricultural and Food Chemistry 2007, 55(19):7962-7967.
95. Roberfroid M.B. Functional foods. Concept to product 2000, Woodhead Publishing Limited, Boca Raton, Boston, New York, Washington, DC. 1st ed. G.R. Gibson, C.M. Williams (Eds.).
96. 2-caused oxidative stress and renal damage. Food Chemistry 2012, 135:2359-2365.
97. Romay C., Armesto J., Remirez D., Gonzalez R., Ledon N., García I. Antioxidant and anti-inflammatory properties of C-phycocyanin from blue-green algae. Inflammation Research 1998, 47:36-41.
98. Romay C., Gonzalez R., Pizarro M., Lissi E. Kinetics of C-phycocyanin reaction with hypochlorite. Journal of Protein Chemistry 2000, 19(2):151-155.
99. Romay C., Ledón N., Gonzalez R. Further studies on anti-inflammatory activity of phycocyanin in some animal models of inflammation. Inflammation Research 1998, 47:334-338.
100. Sabarinathan K.G., Ganesan G. Antibacterial and toxicity evaluation of C-phycocyanin and cell extract of filamentous freshwater cyanobacterium-Westiellopsis sps. European Review for Medical and Pharmacological Sciences 2008, 12:79-82.
101. Samaranayaka A.G.P., Li-Chan E.C.Y. Food-derived peptidic antioxidants: A review of their production, assessment, and potential applications. Journal of Functional Foods 2011, 3(4):229-254.
102. Samsonoff W.A., MacColl R. Biliproteins and phycobilisomes from cyanobacteria and red algae at the extremes of habitat. Archives of Microbiology 2001, 176:400-405.
103. Sathyasaikumar K.V., Swapna I., Reddy P.V.B., Murthy C.R.K., Roy K.R., Dutta Gupta A., Senthilkumaran B., Reddanna P. Co-administration of C-phycocyanin ameliorates thioacetamide-induced hepatic encephalopathy in Wistar rats. Journal of the Neurological Sciences 2007, 252(1):67-75.
104. Satyanarayana L., Patel A., Mishra S., Ghosh P.K., Suresh C.G. Crystal structure of C-phycocyanin from Phormidium, Lyngbya Spp. (marine) and Spirulina Sp. ND Spirulina Sp. (fresh water) shows two different ways of energy transfer between two hexamers Accessed 29.04.14. http://www.rcsb.org/pdb/explore/biologyAndChemistry.do?structureId=2UUL.
105. Scheer H., Zhao K.-H. Biliprotein maturation: The chromophore attachment. Molecular Microbiology 2008, 68(2):263-276.
106. Schirmer T., Bode W., Huber R., Sidler W., Zuber H. X-ray crystallographic structure of the light-harvesting biliprotein C-phycocyanin from the thermophilic cyanobacterium Mastigocladus laminosus and its resemblance to globin structures. Journal of Molecular Biology 1985, 184(2):257-277.
107. Shanab S.M., Mostafa S.S., Shalaby E.A., Mahmoud G.I. Aqueous extracts of microalgae exhibit antioxidant and anticancer activities. Asian Pacific Journal of Tropical Biomedicine 2012, 2(8):608-615.
108. Shen G., Saunée N.A., Williams S.R., Gallo E.F., Schluchter W.M., Bryant D.A. Identification and characterization of a new class of bilin lyase: The cpcT gene encodes a bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the beta-subunit of phycocyanin in Synechococcus sp. PCC 7002. The Journal of Biological Chemistry 2006, 281(26):17768-17778.
109. Shen G., Schluchter W.M., Bryant D.A. Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric phyococyanobilin lyase specific for beta-phycocyanin and allophycocyanin subunits. The Journal of Biological Chemistry 2008, 283(12):7503-7512.
110. Shukia S.P., Singh J.S., Kashyap S., Giri D.D., Kashyap A.K. Antarctic cyanobacteria as a source of phycocyanin: An assessment. Indian Journal of Marine Sciences 2008, 37(4):446-449.
111. Silveira S.T., Quines L.K.D.M., Burkert C.A.V., Kalil S.J. Separation of phycocyanin from Spirulina platensis using ion exchange chromatography. Bioprocess and Biosystems Engineering 2008, 31:477-482.
112. Sloth J.K., Wiebe M.G., Eriksen N.T. Accumulation of phycocyanin in heterotrophic and mixotrophic cultures of the acidophilic red alga Galdieria sulphuraria. Enzyme and Microbial Technology 2006, 38:168-175.
113. Soni B., Trivedi U., Madamwar D. A novel method of single step hydrophobic interaction chromatography for the purification of phycocyanin from Phormidium fragile and its characterization for antioxidant property. Bioresource Technology 2008, 99:188-194.
114. Srivastava A.K. Assessment of salinity-induced antioxidative defense system of diazotrophic cyanobacterium Nostoc muscorum. Journal of Microbiology and Biotechnology 2010, 20(11):1506-1512.
115. Subhashini J., Mahipal S.V.K., Reddy M.C., Mallikarjuna Reddy M., Rachamallu A., Reddanna P. Molecular mechanisms in C-phycocyanin induced apoptosis in human chronic myeloid leukemia cell line-K562. Biochemical Pharmacology 2004, 68(3):453-462.
116. Sun L., Wang S., Qiao Z. Chemical stabilization of the phycocyanin from cyanobacterium Spirulina platensis. Journal of Biotechnology 2006, 121(4):563-569.
117. Sun Y., Zhang J., Yan Y., Chi M., Chen W., Sun P., Qin S. The protective effect of C-phycocyanin on paraquat-induced acute lung injury in rats. Environmental Toxicology and Pharmacology 2011, 32:168-174.
118. 2+ by cyanobacteria in aqueous media. Chemical Communications 2009, 2496-2498. 10.1039/b821687h.
119. Šliumpaite I., Venskutonis P.R., Murkovic M., Pukalskas A. Antioxidant properties and polyphenolics composition of common hedge hyssop (Gratiola officinalis L.). Journal of Functional Foods 2013, 5:1927-1937.
120. Tahergorabi R., Matak K.E., Jaczynski J. Fish protein isolate: Development of functional foods with nutraceutical ingredients. Journal of Functional Foods 2014, 10.1016/j.jff.2014.05.006.
121. Tang K., Zeng X.-L., Yang Y., Wang Z.-B., Wu X.-J., Zhou M., Noy D., Scheer H., Zhao K.-H. A minimal phycobilisome: Fusion and chromophorylation of the truncated core-membrane linker and phycocyanin. Biochimica et Biophysica Acta 2012, 1817:1030-1036.
122. Tapia G., Galetovic A., Lermp E., Pino E., Eduardo L. Singlet oxygen-mediated photobleaching of the prosthetic group in hemoglobins and C-phycocyanin. Photochemistry and Photobiology 1999, 70(4):499-504.
123. Taylor H.R. Epidemiology of age-related cataract. Eye (London, England) 1999, 13:445-448.
124. Tenore G.C., Novellino E., Basile A. Nutraceutical potential and antioxidant benefits of red pitaya (Hylocereus polyrhizus) extracts. Journal of Functional Foods 2012, 4(1):129-136.
125. Thangam R., Suresh V., Asenath Princy W., Rajkumar M., Senthilkumar N., Gunasekaran P., Rengasamy R., Anbazhagan C., Kaveri K., Kannan S. C-Phycocyanin from Oscillatoria tenuis exhibited an antioxidant and in vitro antiproliferative activity through induction of apoptosis and G0/G1 cell cycle arrest. Food Chemistry 2013, 140:262-272.
126. Thanh-Sang V., BoMi R., Se-Kwon K. Purification of novel anti-inflammatory peptides from enzymatic hydrolysate of the edible microalgal Spirulina maxima. Journal of Functional Foods 2013, 5:1336-1346.
127. Vadiraja B.B., Gaikwad N.W., Madyastha K.M. Hepatoprotective effect of C-phycocyanin: Protection for carbon tetrachloride and R-(+)-pulegone-mediated hepatotoxicity in rats. Biochemical and Biophysical Research Communications 1998, 249(2):428-431.
128. Velazquez-López L., Santiago-Díaz G., Nava-Hernandez J., Muñoz-Torres A.V., Medina-Bravo P., Torres-Tamayo M. Mediterranean-style diet reduces metabolic syndrome components in obese children and adolescents with obesity. BMC Pediatrics 2014, 14:175. 10.1186/1471-2431-14-175.
129. Vulíc J., Ćebovic T., Čanadanovic-Brunet J., Ćetkovic G., Čanadanovic V., Djilas S., Tumbas V. In vivo and in vitro antioxidant effects of beetroot pomace extracts. Journal of Functional Foods 2014, 6:168-175.
130. Wang C., Wang X., Wang Y., Zhou T., Bai Y., Li Y., Huang B. Photosensitization of phycocyanin extracted from Microcystis in human hepatocellular carcinoma cells: Implication of mitochondria-dependent apoptosis. Journal of Photochemistry and Photobiology. B, Biology 2012, 117:70-79.
131. Wang L., Qu Y., Fu X., Zhao M., Wang S., Sun L. Isolation, purification and properties of an R-Phycocyanin from the phycobilisomes of a marine red macroalga Polysiphonia urceolata. PLoS ONE 2014, 9(2):e87833. 10.1371/journal.pone.0087833.
132. Wiriyaphan C., Chitsomboon B., Roytrakul S., Yongsawadigul J. Isolation and identification of antioxidative peptides from hydrolysate of threadfin bream surimi processing byproduct. Journal of Functional Foods 2013, 5:1654-1664.
133. Womick J.M., Moran A.M. Nature of excited states and relaxation mechanisms in C-phycocyanin. The Journal of Physical Chemistry B 2009, 113:15771-15782.
134. Wu L.-C., Lin Y.-Y., Yang S.-Y., Weng Y.-T., Tsai Y.-T. Antimelanogenic effect of c-phycocyanin through modulation of tyrosinase expression by upregulation of ERK and downregulation of p38 MAPK signaling pathways. Journal of Biomedical Science 2011, 18(74). 10.1186/1423-0127-18-74.
135. Yoshida A., Takagaki Y., Nishimune T. Enzyme immunoassay for phycocyanin as the main component of spirulina color in foods. Bioscience, Biotechnology, and Biochemistry 1996, 60(1):57-60.
136. Zhan-Ping Z., Lu-Ning L., C X.-L., Jin-Xia W., Min C., Yu-Zhang Z., Bai-Cheng Z. Factors that effect antioxidant activity of c-phycocyanins from Spirulina platensis. Journal of Food Biochemistry 2005, 29:313-322.
137. Zhang H., Chen T., Jiang J., Wong Y.-S., Yang F., Zheng W. Selenium-containing allophycocyanin purified from selenium-enriched Spirulina platensis attenuates AAPH-induced oxidative stress in human erythrocytes through inhibition of ROS generation. Journal of Agricultural and Food Chemistry 2011, 59:8683-8690.
138. Zheng J., Inoguchi T., Sasaki S., Maeda Y., McCarty M.F., Fujii M., Ikeda N., Kobayashi K., Sonoda N., Takayanagi R. Phycocyanin and phycocyanobilin from Spirulina platensis protect against diabetic nephropathy by inhibiting oxidative stress. American Journal of Physiology - Regulatory Integrative and Comparative Physiology 2013, 304:R110-R120.
139. Zhuang H., Tang N., Yuan Y. Purification and identification of antioxidant peptides from corn gluten meal. Journal of Functional Foods 2013, 5:1810-1821.
140. Zilinskas B.A., Greenwald L.S. Mini review. Phycobilisome structure and function. Photosynthesis Research 1986, 35(10):7-35.