Cysteine rich cyanopeptide β2 from Spirulina fussiformis exhibits plasmid DNA pBR322 scission prevention and cellular antioxidant activity

Indian Journal of Experimental Biology

Volumn 48, Issue 5, 2010, Pages 486-493

  Madhyastha, H ^a,b   Vatsala, T M ^b,c  

^a Shri AMM Murugappa Chettiar Research Centre   (India)

^b UNIVERSITY OF MIYAZAKI   (Japan)

^c TICEL Biopark Taramani   (India)

Author keywords

Antioxidation; C phycocyanin peptide; DNA scission activity; Spirulina fussiformis

Indexed keywords

1,1 DIPHENYL 2 PICRYLHYDRAZYL; 2,2-DIPHENYL-1-PICRYLHYDRAZYL; ANTIOXIDANT; BACTERIAL DNA; BIPHENYL DERIVATIVE; CYSTEINE; PEPTIDE FRAGMENT; PHYCOCYANIN; PICRIC ACID; REACTIVE OXYGEN METABOLITE; DNA SUPERCOILING;

AMINO ACID SEQUENCE; ARTICLE; CELL LINE; CHEMISTRY; DNA DAMAGE; DNA SUPERCOILING; DRUG ANTAGONISM; DRUG EFFECT; DRUG SCREENING; FIBROBLAST; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MEDICINAL PLANT; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; PLASMID; RADIATION EXPOSURE; SPIRULINA; ULTRAVIOLET RADIATION; ANTAGONISTS AND INHIBITORS; DRUG EFFECTS; RADIATION RESPONSE;

AMINO ACID SEQUENCE; ANTIOXIDANTS; BIPHENYL COMPOUNDS; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; DNA DAMAGE; DNA, BACTERIAL; DNA, SUPERHELICAL; DRUG EVALUATION, PRECLINICAL; FIBROBLASTS; HUMANS; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PEPTIDE FRAGMENTS; PHYCOCYANIN; PICRATES; PLANTS, MEDICINAL; PLASMIDS; REACTIVE OXYGEN SPECIES; SPIRULINA; ULTRAVIOLET RAYS;

SPIRULINA;

EID: 77954251027     PISSN: 00195189     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Borowitzka M A, Microalgae as sources of pharmaceuticals and other biologically active compounds, J Appl Phycol, 7 (1995) 3.
2. McCarty M F, Clinical potential of Spirulina as a source of phycocyanobilin, J Med Food, 10 (2007) 566.
3. Soni B, Trivedi U & Madamwar D, A novel method of single step hydrophobic interaction chromatography for the purification of phycocyanin from Phormidium fragile and its characterization for antioxidant property. Bioresour Technol, 99 (2008) 188.
4. Suestuna K & Chen J R, Identification of antihypertensive peptides from peptic digest of two microalgae, Chlorella vulgaris and Spirulina platensis, Marine Biotech, 3 (2001) 305.
5. Morris H.J, Bermudez R.C, Carrillo O & Alonso M E, Are the peptide sequences encrypted in food Chlorella protein a possible explanation for the immunostimulatory effects of microalgal supplements?, Med Hypotheses, 70 (2008) 896.
6. Madhyastha HK & Vatsala TM, Pigment production in Spirulina fussiformis in different photophysical conditions, Biomol Eng, 24 (2007) 135.
7. Madhyastha H K, Shivashankari S & Vatsala T M, C-phycocyanin from Spirulina fussiformis exposed to blue light demonstrates higher efficacy of in vitro antioxidant activity, Biochemical Eng J, 43 (2009) 221.
8. Madhyastha H K, Radha, K S, Suzuki M, Omura S & Maruyama M, Purification of c-phycocyanin from Spirulina fussiformis and its effect on the induction of urokinase-type plasminogen activator from calf pulmonary endothelial cells, Phytomedicine, 13 (2006) 564.
9. Edmen, P, Method for determination of the amino acid sequence in peptides, Acta Chemica Scandinavica, 4 (1950) 283.
10. Blois M S, Free radicals in biological systems, Science, 132 (1960) 306.
11. Antolovich M, Prenzler P D, Patsalides E, McDonald S & Robards K, Methods for testing antioxidant activity, Analyst, 127 (2002) 183.
12. Oyaizu M, Studies on product of browning reaction prepared from glucose amine, Jpn J Nutr, 44 (1986) 307.
13. Ghiselli A, Serafini M, Maiani G, Azzini E & Ferro-Luzzi A, A fluorescence-based method for measuring total plasma antioxidant capability, Free Radic Biol Med, 18 (1995) 29.
14. Maxam A M & W Gilbert, A new method for sequencing DNA, Proc Natl Acad Sci U S A, 74 (1977) 560.
15. Niidome T, Ohmori N, Ichinose A, Wada A, Mihara H, Hirayama H & Aoyagi A, Binding of cationic alpha-helical peptides to plasmid DNA and their gene transfer abilities into cells, J Biol Chem, 272 (1997) 15302.
16. Liang C C, Park A Y & Guan J L, In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro, Nature Protocols, 2 (2007) 329.
17. Schlesier K & Harwat M, Assessment of antioxidant activity by using different in vitro methods, Free Radic Res, 36 (2002) 177.
18. Diamond G, Zasloff M, Eck H, Brasseur M, Maloy W L & Bevins C L, Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa: Peptide isolation and cloning of a cDNA, Proc Natl Acad Sci U S A, 88 (1991) 3952.
19. Duan X J, Zhang W W, Li X M & Wang B G, Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata, Food Chem, 95 (2006) 37.
20. Bohlmann H, Clausen S, Behnke S, Giese H, Hiller C, Reimann-Philipp U, Schrader G, Barkholt V & Apel K, Leaf-specific thionins of barley-a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defense mechanism of plants, EMBO J, 7 (1988) 1559.
21. Cao G & Prior R L, Measurement of oxygen radical absorbance capacity in biological samples, Methods Enzymol, 299 (1999) 50.
22. Cao G & Prior R L, Comparison of different analytical methods for assessing total antioxidant capacity of human serum, Clin Chem, 44 (1998) 1309.
23. Elias R J, Kellerthy S S & Decker E A, Antioxidant activity of protein and peptides, Crt. Rev. Food Sci. Nutr, 48 (2008) 430.
24. Salim S A, The role of oxygen-derived free radicals in the management of venous (Varicose) ulceration: A new approach, World J Surg, 15 (1991) 264.
25. Loft S, Hungeingh P, Danielsen T, Mikkelsen L, Risom L & Forchhammer P, Biomarkers of oxidative damage to DNA and repair, Biochemical Society Transactions, 36 (2008) 1071.
26. Rahman A, Fazal F, Greensill J, Ainley K, Parish J H & Hadi S M, Strand scission in DNA induced by dietary flavonoids: Role of Cu(I) and oxygen free radicals and biological consequences of scission, Mol Cell Biochem, 111 (1992) 3.
27. Oliveri M, Daga A, Cantoni C, Lunardi C, Millo R & Puccetti A, DNase I mediates internucleosomal DNA degradation in human cells undergoing drug-induced apoptosis, Eur J Immunol, 31 (2001) 743.
28. Spencer J P, Jenner A, Chimel K, Aruoma O I, Cross C E, Wu R & Halliwell B, DNA damage in human respiratory tract epithelial cells: Damage by gas phase cigarette smoke apparently involves attack by reactive nitrogen species in addition to oxygen radicals, FEBS Lett, 375 (1995) 179.